Glomerular loss after arteriovenous and arterial clamping for renal warm ischemia in a swine model

Bechara, Gustavo Ruschi; Damasceno-Ferreira, José Aurelino; Abreu, L.; Costa, Waldemar S.; Sampaio, Francisco J.B.; Pereira‐Sampaio, Marco A.; Souza, Diogo Benchimol de

doi:10.1590/s0102-865020160110000008

Cited by 9 publications

(14 citation statements)

References 21 publications

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“…The results indicated that the volumes of the renal tubules were augmented, which is in accordance with the literature [21]. Unbiased stereology has been used for studying the number of glomeruli in different conditions [12, 14, 16, 39] and is considered a highly sensitive method [40]. Thus, it may be thought that the obstructed kidney did not promote alterations in the number of glomeruli but in the tubular structure of contralateral kidney.…”

Section: Discussionsupporting

confidence: 85%

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Does a Ureteral Obstruction Affect the Contralateral Kidney Morphology? A Stereological Analysis in a Rodent Model

et al. 2018

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Objective: To investigate, in a rodent model, the influence of an obstructed kidney/ureter on the contralateral organ morphology. Methods: Thirty-six rats were randomly assigned into the following groups: untreated ureteral obstruction group (UO-U, n = 10), submitted to a ligature of the ureter at day 0; UO group (at day 0) followed by nephrectomy after 24 h (UO-N, n = 8); nephrectomy group (N, n = 9), submitted to nephrectomy at day 0; and sham group (S, n = 9), submitted to simulated surgery at day 0. All these procedures were performed on the left kidney/ureter. All animals were euthanized 30 days after surgery, and the right kidneys were collected for stereological analysis. Data were compared using a one-way analysis of variance with Bonferroni’s post test, considering p < 0.05 as significant. Results: The kidney volume, weight, and cortical volume were augmented in groups UO-N and N, compared to group S. Kidneys from the UO-U group showed only a moderate augmentation in kidney weight, with a reduction in the cortical-medullar ratio, compared to the kidneys in group S. No differences in the glomerular volumetric density, volume-weighted glomerular volume, and number of glomeruli were observed among the right kidneys of the studied groups. Conclusion: An obstructed kidney/ureter is more prejudicial to the contralateral kidney morphology than an absent kidney.

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Section: Discussionsupporting

confidence: 85%

“…Analyses of Vv(glom) and VWGV were performed using the ImageJ software version 1.46r (NIH, Bethesda, MD, USA). An estimation of the total number of glomeruli per kidney was calculated by multiplying the CV by the Vv(glom) and dividing the result by the VWGV [14, 16]. …”

Section: Methodsmentioning

confidence: 99%

Does a Ureteral Obstruction Affect the Contralateral Kidney Morphology? A Stereological Analysis in a Rodent Model

et al. 2018

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“…General anesthesia was induced with propofol (3mg/kg, IV) and maintained with isoflurane (2% in oxygen, given by an endotracheal tube). Under general anesthesia and by using aseptic technique, the surgical procedure involved transperitoneal laparoscopic access, as previously used in pigs 15,16 . The left kidney was completely exposed by dissection.…”

Section: ■ Methodsmentioning

confidence: 99%

“…The volume weighted mean glomerular volume (VWGV), was estimated by using the point-sampled intercepts method 14,16,18,22,23 , analyzing 50 glomeruli per kidney.…”

Section: ■ Introductionmentioning

confidence: 99%

The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model

et al. 2017

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The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model 1 Acta Cir. Bras. 2017;32(5):334-341 Abstract Purpose: To investigate the glomerular number after different warm ischemia times. Methods: Thirty two pigs were assigned into four groups. Three groups (G10, G20, and G30) were treated with 10, 20, and 30 minutes of left renal warm ischemia. The sham group underwent the same surgery without renal ischemia. The animals were euthanized after 3 weeks, and the kidneys were collected. Right kidneys were used as controls. The kidney weight, volume, cortical-medullar ratio, glomerular volumetric density, volume-weighted mean glomerular volume, and the total number of glomeruli per kidney were obtained. Serum creatinine levels were assessed pre and postoperatively. Results: Serum creatinine levels did not differ among the groups. All parameters were similar for the sham, G10, and G20 groups upon comparison of the right and left organs. The G30 group pigs' left kidneys had lower weight, volume, and cortical-medullar ratio and 24.6% less glomeruli compared to the right kidney. A negative correlation was found between warm ischemia time and glomerular number. Conclusions: About one quarter of glomeruli was lost after 30 minutes of renal warm ischemia. No glomeruli loss was detected before 20 minutes of warm ischemia. However, progressive glomerular loss was associated with increasing warm ischemia time.

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“…In this regard, an objective assessment of the glomeruli may accurately identify the functional units that were damaged during WI. Stereological methods such as those used in our study reduce sampling bias and may increase accuracy in identifying irreversible kidney damage after ischemic injury [14,18,20] .…”

Section: Discussionmentioning

confidence: 99%

Glomerular Morphology and Renal Function after Warm Ischemia by Main Artery or Selective Clamping in a Porcine Model

Bechara

Damasceno-Ferreira²,

Abreu³

et al. 2017

Urol Int

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Objective: The study aimed to evaluate the ischemic and non-ischemic areas after selective arterial occlusion by using stereological analysis of glomeruli, and to compare them with main arterial clamping and sham-operated animals. Materials and Methods: Twenty-four male pigs were used in the study. The animals were divided into 3 groups with 8 animals in each as follows: group sham, submitted to laparoscopic dissection of the renal pedicle but not submitted to ischemia; group arterial (A), submitted to left renal artery clamping; and group selective (S), submitted to left renal artery caudal branch occlusion. Groups A and S underwent 30 min of warm ischemia. Left and right kidneys were collected after 21 days and renal fragments were processed for stereological evaluation. Glomerular volume density (Vv[glom]), mean glomerular volume (MGV), and glomerular density were measured. Serum creatinine and urea were assessed preoperatively, 10 days after surgery, and before euthanasia. Results: There was no significant difference among groups with regard to renal function. Renal weight and volume were similar among groups. Also, no difference was observed between the groups with regard to Vv[glom], MGV, and glomerular density, both when compared to its right control or when left kidneys were compared. Conclusions: Selective arterial clamping technique was neither superior nor inferior to main artery clamping.

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Glomerular loss after arteriovenous and arterial clamping for renal warm ischemia in a swine model

Cited by 9 publications

References 21 publications

Does a Ureteral Obstruction Affect the Contralateral Kidney Morphology? A Stereological Analysis in a Rodent Model

Does a Ureteral Obstruction Affect the Contralateral Kidney Morphology? A Stereological Analysis in a Rodent Model

The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model

Glomerular Morphology and Renal Function after Warm Ischemia by Main Artery or Selective Clamping in a Porcine Model

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