Glucan injection or bath exposure given alone or in combination with a bacterin enhance the non-specific defence mechanisms in rainbow trout (Oncorhynchus mykiss)

Jeney, Galina; Anderson, D.P.

doi:10.1016/0044-8486(93)90416-v

Cited by 109 publications

(79 citation statements)

References 16 publications

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“…Regarding the increased ROSs production by granulocytes, it has been considered an indicator of the innate immune system activation (Jeney and Anderson, 1993;Jorgensen and Robertsen, 1995). In this way, pacu challenged by A. hydrophila have shown improvement in ROSs production and increased protection against pathogens, assessed by the respiratory burst assay.…”

Section: Resultsmentioning

confidence: 99%

Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus

et al. 2013

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The present study evaluated the assay to quantify the respiratory burst activity of blood leukocytes of pacu as an indicator of the innate immune system, using the reduction of nitroblue tetrazolium (NBT) to formazan as a measure of the production of reactive oxygen species (ROS). In order to assess the accuracy of the assay, fish were challenged by Aeromonas hydrophila and sampled one week after challenge. The A. hydrophila infection increased the leukocyte respiratory burst activity. The protocol showed a reliable and easy assay, appropriate to determine the respiratory burst activity of blood leukocytes of pacu, a neotropical fish, in the present experimental conditions. Keywords: immune methodology, fish immunology, cellular immunity. Atividade respiratória de leucócitos como indicador de imunidade inata de pacu (Piaractus mesopotamicus)Resumo O presente estudo avaliou o ensaio para quantificar a atividade respiratória dos leucócitos do sangue de pacu como um indicador do sistema imune inato, usando a redução do nitroazul tetrazólio (NBT) a formazan como medida da produção de espécies reativas de oxigênio (EROs). Para avaliar a precisão do ensaio, peixes foram desafiados por Aeromonas hydrophila e amostrados uma semana após o desafio. A infecção com A. hydrophila aumentou a atividade respiratória dos leucócitos. O protocolo se mostrou confiável e de fácil aplicação, apropriado para determinar a atividade respiratória de leucócitos do sangue do pacu, peixe neotropical, nas condições experimentais apresentadas.Palavras-chave: metodologia, imunidade de peixe, imunidade mediada por células.

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Section: Resultsmentioning

confidence: 99%

Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus

et al. 2013

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“…Recently, several groups have demonstrated that /-glucans also stimulate the non-specific antibacterial defense of fish (Chen &Ainsworth, 1992 andJeney &Anderson, 1993). Another study has demonstrated that head kidney macrophages from glucan-treated rainbow trout, Oncorhynchus mykiss (walbaum), have an elevated bactericidal capacity (Jcrgensen et al, 1993).…”

Section: Mostafa a Ei-feki Et Almentioning

confidence: 99%

Changes in the Haematological Parameters of Carp, Cyprinus Carpjo L., Induced by Laminarin and Nigella Sativa Oil During the Motile Aeromonas Septicemia Disease

El-Feki¹,

Fahim

Gad

et al. 2000

Egypt. J. of Aquatic Biolo. and Fish.

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Aeromonas hydrophila is responsible for heavy mortality to f»h stocks specially under culturing conditions. It is usually associated with the development of fish bacterial haemorrhagic septicemia. Therefore, the need for protection and treatment programmes is increasingly called on to avoid and/or overcome disease outbreaks in fishery populations.The haematological studies were carried out including the total erythrocytic count, haemoglobin estimation, packed cell volume "PCV 11 and the total and differential leucocytic counts. Various derived values can be calculated from these results, such as mean corpuscular volume "MCV", mean cell haemoglobin "MCH" and mean corpuscular haemoglobin concentration "MCHC".The haematologjcal changes in the blood of common carp induced after the i.p. injection with Aeromonas hydrophila pathogen weie 44 Mostafa A. El-Feki. et al blood cells, haemoglobin concentration and haematocrit values. These increments were due to the tissue hypoxia resulting from erythropenia caused by Aeromonas hydrophila infection. Treatment of the infected fishes with both laminarin and N. sativa showed a decrease in the RBCs count and haemoglobin concentration to the range of subnormal level A marked decrease in the RBCs count beyond the normal value and a noticeable increase in the haemoglobin concentration were noticed at the 5 lh week and the 4 th and 5 th weeks, respectively in the K sativa treated group. The PCV values were changed in a fluctuated manner parallel to the changes in the quantities of circulating RBCs and/or modified erythrocyte cellular volume.In the protected groups, the haematological changes reflected the same effects of the immunopotentiators recorded in the treated ones. Also, following the challenge with bacteria, there was a decrease in the RBCs count, haemoglobin concentration and PCV values. Furthermore, the blood indices were calculated to interrupt and to give valuable support for the haematological results, The total leucocyte counts was increased after the challenge with bacteria, then a further elevation was recorded during the time of treatment with both of the immunopotentiators. Also, in the protected groups, the leucocyte counts was increased with laminarin and N. sativa injection. While it remained nearly unchanged following the infection with bacteria in the laminarin protected group, it was decreased in the N. sativa protected one.The increased leucocyte counts were accompanied by an increase in the neutrophils and a decrease in the lymphocytes following the infection with bacteria and injection with N, sativa in both protected and treated groups, Meanwhiles, in the groups injected with laminarin, the neutrophil and lymphocyte counts were nearly fluctuated around the control values. In addition, monocytes were increased in all the studied Changes in the haemattriogkai parameters of Cyprmus awpio during the motile Aeromonas septicemia diacase 45 control values. In addition, monocytes were increased in all the studied groups except in the laminarin treated o...

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“…Betaglucans bind to specifi c cell surface receptors of macrophages and neutrophilic granulocytes (Brown and Gordon, 2003). Beta-glucans are capable of enhancing the innate immunity by increasing plasma lysozyme and complement and stimulate the phagocytic activity of macrophages in several cultured fi sh species (Yano et al, 1991;Chen and Ainsworth, 1992;Jorgensen et al, 1993;Galeotti, 1998;Jeney and Anderson, 1993). Beta-glucans enhance fi sh resistance against bacterial and viral infections by means of eff ective stimulation of nonspecifi c cellular and humoral immune functions and have a proven protective eff ect against fi sh bacterial pathogens including A. salmonicida (Nikl et al, 1993).…”

Section: Immunostimulantsmentioning

confidence: 99%

Preventive and Prophylactic Measures in Intensive Salmonid Fish Breeding - a Review

Palíková¹,

Navrátil²,

Navrátil³

et al. 2015

Acta Univ. Agric. Silvic. Mendelianae Brun.

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Aquaculture represents the fastest growing animal husbandry in many countries word-wide. Intensive fish production has increased the risk of infectious diseases all over the world. Bacterial pathogens probably cause most disease problems in aquaculture. Most infectious bacterial diseases in commercial fish species continue to be controlled by treatment with chemotherapeutic agents although they represent a potential environmental hazard and could also have negative effects on the quality of the final product. The most important problem of antibiotic treatment is the increase in resistant bacteria, the presence of their residues in aquaculture products and the resulting threat to human health. Therefore, it is most effective to prevent the introduction or onset of the disease. Beside preventing the introduction of pathogens, good water quality, reduction of environmental stressors, adequate nutrition and immunization, many substances improving the health status of fish, such as immunostimulants, probiotics, prebiotics, symbiotics and so on, are used in fish breeding. The present review gives an overview of preventive and prophylactic substances which can be used in salmonid fish breeding to improve the health status of fish.

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Glucan injection or bath exposure given alone or in combination with a bacterin enhance the non-specific defence mechanisms in rainbow trout (Oncorhynchus mykiss)

Cited by 109 publications

References 16 publications

Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus

Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus

Changes in the Haematological Parameters of Carp, Cyprinus Carpjo L., Induced by Laminarin and Nigella Sativa Oil During the Motile Aeromonas Septicemia Disease

Preventive and Prophylactic Measures in Intensive Salmonid Fish Breeding - a Review

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