Glucoamylase absorption and desorption process

Abstract

This paper reports the study of glucoamylase absorption and desorption processes on spherical particles constituting acrylic supports. The kinetic (reaction order, half-life of the reaction, reaction rate constant), and thermodynamic parameters (activation energy, pre-exponential factor) of the glucoamylase immobilization reaction dynamics inside the acrylic supports have been studied. Hindrance of the penetrants (protein molecules), which defeats the polymer's cohesive forces and passes through the diffusional barriers towards the sites available to receive it, denotes the influence of the diffusion phenomena, and might be expressed by the values of the effective diffusion coefficient and of the energetic parameters, as well. The desorption dynamics of glucoamylase in specific buffer concentration gradients and temperature are dependent on the eluent volume and on the protein eluate.

Recovery of gangliosides from aqueous solutions on styrene-divinylbenzene copolymer columns

“…The recovery decreased sharply above the 5 mg that seems to be the saturation level for 1 ml of copolymer loaded with gangliosides. The very low recovery above this amount can be explained by the competition for adsorption of lipids such as gangliosides when a steric hindrance occurs as a result of the diffusional barriers on the path to the active sites (16).…”