Glucose and galactose regulate intestinal absorption of cholesterol

Play, Barbara; Salvini, Séverine; Haikal, Ziad; Charbonnier, Monique; Harbis, Amandine; Roussel, M.; Lairon, Denis; Jourdheuil-Rahmani, Dominique

doi:10.1016/j.bbrc.2003.08.150

Cited by 20 publications

(11 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The TC7 cells, a clone of human intestinal cell-line Caco-2, were used between passages 30 and 50 as previously described [28,29]. Stocks of cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 25 mM glucose, supplemented with 1% nonessential amino acids and 20% heat-inactivated (30 min, 56°C) fetal calf serum in a 10% CO 2 humidified incubator at 37°C.…”

Section: Cell Culture Conditionsmentioning

confidence: 99%

“…TCA-free CL donors were also prepared; one part was used without treatment (0) and the other part was sonicated for 10 min at 4 dB (0s) (Sonifier 250, Branson, Danbury, CT, USA). CL donors were added to the apical side and cells were incubated for 3 h at 37°C, as previously published [28]. At the end of the experiment, the radioactivities of the basolateral and apical media were measured.…”

Section: Absorption Measurementmentioning

confidence: 99%

See 1 more Smart Citation

NPC1L1 and SR‐BI are Involved in Intestinal Cholesterol Absorption from Small‐Size Lipid Donors

Haikal¹,

Play²,

Landrier³

et al. 2008

Lipids

Self Cite

View full text Add to dashboard Cite

In the human intestinal content after a meal, cholesterol is dispersed in a complex mixture of emulsified droplets, vesicles, mixed micelles and precipitated material. The aim of this study was to determine the contribution of the main intestinal cholesterol transporters (NPC1L1, SR-BI) to the absorption processes, using different cholesterol-solubilizing donors. Cholesterol donors prepared with different taurocholate concentrations were added to an apical medium of differentiated TC7/Caco-2 cells. As the taurocholate concentrations increased, cholesterol donor size decreased (from 712 to 7 nm in diameter), which enhanced cholesterol absorption in a dose-dependent manner (38-fold). Two transport processes were observed: (1) absorption from large donors exhibited low-capacity transport with no noticeable transporter contribution; (2) efficient cholesterol absorption occurs from small lipid donors (

show abstract

Section: Cell Culture Conditionsmentioning

confidence: 99%

Section: Absorption Measurementmentioning

confidence: 99%

NPC1L1 and SR‐BI are Involved in Intestinal Cholesterol Absorption from Small‐Size Lipid Donors

Haikal¹,

Play²,

Landrier³

et al. 2008

Lipids

Self Cite

View full text Add to dashboard Cite

show abstract

“…This indicated that EGB had a galactose-lowering effect. Galactose and glucose are associated with the intestinal absorption of cholesterol by regulating the intestinal cholesterol transporter hSR-BI [53] . There are two sources of galactose available to humans [54] .…”

Section: Effects On Energy Metabolismmentioning

confidence: 99%

Application of GC/MS-based metabonomic profiling in studying the lipid-regulating effects of Ginkgo biloba extract on diet-induced hyperlipidemia in rats

Zhang

Wang

et al. 2009

Acta Pharmacol Sin

View full text Add to dashboard Cite

Aim: To evaluate the lipid-regulating effects of extract from Ginkgo biloba leaves (EGB) using pharmacological methods and metabonomic profiling in a rat model of diet-induced hyperlipidemia. Methods: EGB was orally administered at a dose level of 40 mg/kg in both the EGB-prevention and -treatment groups. All rat samples obtained were examined for known and potential biomarkers and enzyme activity using commercial assay kits and GC/MS-based metabonomic profiling coupled with principal component analysis (PCA). Results: The data obtained from the assay kits indicated that EGB reduced total cholesterol and low density lipoprotein cholesterol levels and increased high density lipoprotein cholesterol levels in rat plasma obtained from both the EGB-prevention and -treatment groups compared with those of the diet-induced hyperlipidemia group. EGB also increased the activities of lipoprotein lipase and hepatic lipase and excretion of fecal bile acid in rats from the EGB-prevention and -treatment groups. Using GC/MS-based metabonomic analysis, more than 40 endogenous metabolites were identified in rat plasma. PCA of rat plasma samples obtained using GC/ MS produced a distinctive separation of the four treatment groups and sampling points within each group. Metabolic changes during hyperlipidemia formation and improvement resulting from EGB treatment were definitively monitored with PCA score plots. Furthermore, elevated levels of sorbitol, tyrosine, glutamine and glucose, and decreased levels of citric acid, galactose, palmitic acid, arachidonic acid, acetic acid, cholesterol, butyrate, creatinine, linoleate, ornithine and proline, were observed in the plasma of rats treated with EGB. Conclusion: EGB exerts multi-directional lipid-lowering effects on the rat metabonome, including limitation of the absorption of cholesterol, inactivation of HMGCoA and favorable regulation of profiles of essential polyunsaturated fatty acid (EFA). Further experiments are warranted to explore the mechanisms of action underlying the lipid-regulating effects of EGB against hyperlipidemia.

show abstract

“…Moreover, a PKC pathway affects the different mechanisms of cell lipid metabolism. It regulates both intestinal cholesterol absorption [87] and possibly FA uptake (unpublished observations) and cholesterol uptake from HDL [93]; it also decreases lipid accumulation in human macrophages [94] and is implicated in the expression levels of lipid transporters such as SR-BI [95], ABCA1 [96] and MTP [97]. Thus, a PKC pathway regulates both cholesterol and glucose uptakes and may be a link between the two metabolic processes.…”

Section: Glucose Regulates Intestinal Lipid Absorptionmentioning

confidence: 97%

“…It has been observed that women who consumed more cholesterol with a low carbohydrate intake had lower concentrations of lowdensity lipoprotein (LDL) cholesterol than women with a high carbohydrate intake [86]. In vitro, a short-term incubation (3 h) of intestinal Caco-2 TC7 cells with glucose on the apical side induced a significant increase of cholesterol uptake in a dose-dependent manner [87]. The mechanism involved in this glucose-induced regulation of uptake process is not yet fully identified.…”

Section: Glucose Regulates Intestinal Lipid Absorptionmentioning

confidence: 98%

Digestible and indigestible carbohydrates: interactions with postprandial lipid metabolism

Lairon

Play

Jourdheuil-Rahmani

2007

The Journal of Nutritional Biochemistry

125

View full text Add to dashboard Cite

Glucose and galactose regulate intestinal absorption of cholesterol

Cited by 20 publications

References 34 publications

NPC1L1 and SR‐BI are Involved in Intestinal Cholesterol Absorption from Small‐Size Lipid Donors

NPC1L1 and SR‐BI are Involved in Intestinal Cholesterol Absorption from Small‐Size Lipid Donors

Application of GC/MS-based metabonomic profiling in studying the lipid-regulating effects of Ginkgo biloba extract on diet-induced hyperlipidemia in rats

Digestible and indigestible carbohydrates: interactions with postprandial lipid metabolism

Contact Info

Product

Resources

About