Glucose- and sucrose-signaling modules regulate the Arabidopsis juvenile-to-adult phase transition

Meng, Lai-Sheng; Bao, Qin-Xin; Mu, Xin-Rong; Chen, Tong; Cao, Xiaoying; Huang, Jinjin; Xue, Li-Na; Liu, Chang-Yue; Fei, Yue; Loake, Gary J.

doi:10.1016/j.celrep.2021.109348

Cited by 33 publications

(14 citation statements)

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“…The cotyledon greening of etiolated seedlings was promoted by endogenous sugars Cytosolic invertase 1/2 (CINV1/2) irreversibly catalyzes Suc into glucose (Glc) and fructose (Frc) (Barratt et al, 2009). Here, we utilized the loss-of-function mutant of CINV1/2, cinv1/2 (sugar signaling/metabolism mutant [Barratt et al, 2009;Meng et al, 2021]), to obtain the endogenous sugars of genetic manipulation.…”

Section: Resultsmentioning

confidence: 99%

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Feedback loop promotes sucrose accumulation in cotyledons to facilitate sugar-ethylene signaling-mediated, etiolated-seedling greening

Chen

Fang

et al. 2022

Cell Reports

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Section: Resultsmentioning

confidence: 99%

“…Subsequently, 1 mL of the reverse transcription reaction was used as template for PCR amplification. The same RNA samples and primers were used for real-time PCR analysis that was performed and statistically analyzed as described (Meng et al, 2021). Kinetic detection of PCR products using Sybrgreen real-time RT-PCR.…”

Section: Quantitative Pcrmentioning

confidence: 99%

Feedback loop promotes sucrose accumulation in cotyledons to facilitate sugar-ethylene signaling-mediated, etiolated-seedling greening

Chen

Fang

et al. 2022

Cell Reports

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“…PtrA/NINV7 was prominently upregulated by cold stress, indicating that it is a cold‐responsive gene. It is well accepted that TFs play a key role in transcriptional regulation of stress‐responsive genes through interacting with the corresponding cis ‐acting elements (CAEs) in the promoters (Li et al ., 2020; Meng et al ., 2020, 2021). In the current study we showed that the A/T‐rich sequences in the MAR region of the promoter are essential for the cold‐induced activation of PtrA/NINV7 , implying that the A/T‐rich sequences may also act as a CAE for orchestrating gene expression in response to cold cues.…”

Section: Discussionmentioning

confidence: 99%

Two AT‐Hook proteins regulate A/NINV7 expression to modulate sucrose catabolism for cold tolerance in Poncirus trifoliata

et al. 2022

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Invertase (INV)-mediated sucrose (Suc) hydrolysis, leading to the irreversible production of glucose (Glc) and fructose (Frc), plays an essential role in abiotic stress tolerance of plants. However, the regulatory network associated with the Suc catabolism in response to cold environment remains largely elusive.Herein, the cold-induced alkaline/neutral INV gene PtrA/NINV7 of trifoliate orange (Poncirus trifoliata (L.) Raf.) was shown to function in cold tolerance via mediating the Suc hydrolysis. Meanwhile, a nuclear matrix-associated region containing A/T-rich sequences within its promoter was indispensable for the cold induction of PtrA/NINV7.Two AT-Hook Motif Containing Nuclear Localized (AHL) proteins, PtrAHL14 and PtrAHL17, were identified as upstream transcriptional activators of PtrA/NINV7 by interacting with the A/T-rich motifs. PtrAHL14 and PtrAHL17 function positively in the cold tolerance by modulating PtrA/NINV7-mediated Suc catabolism. Furthermore, both PtrAHL14 and PtrAHL17 could form homo-and heterodimers between each other, and interacted with two histone acetyltransferases (HATs), GCN5 and TAF1, leading to elevated histone3 acetylation level under the cold stress.Taken together, our findings unraveled a new cold-responsive signaling module (AHL14/17-HATs-A/NINV7) for orchestration of Suc catabolism and cold tolerance, which shed light on the molecular mechanisms underlying Suc catabolism catalyzed by A/NINVs under cold stress.

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“…Among them, flowering is a physiological development process that transforms plants from vegetative growth to reproductive growth, which indicates that miR156/ SPL participates in the regulation of plant age. Exogenous sugars trigger the Arabidopsis juvenile-to-adult phase transition via a miR156A/ SPL module [ 44 ]. Fraxinus mandshurica is a perennial woody plant.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis and molecular dissection of the SPL gene family in Fraxinus mandshurica

Gao

Han

et al. 2022

BMC Plant Biol

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Background SQUAMOSA promoter binding protein-like (SPL) is a unique family of transcription factors in plants, which is engaged in regulating plant growth and development, physiological and biochemical processes. Fraxinus mandshurica is an excellent timber species with a wide range of uses in northeastern China and enjoys a high reputation in the international market. SPL family analysis has been reported in some plants while SPL family analysis of Fraxinus mandshurica has not been reported. Results We used phylogeny, conserved motifs, gene structure, secondary structure prediction, miR156 binding sites, promoter cis elements and GO annotation to systematically analyze the FmSPLs family. This was followed by expression analysis by subcellular localization, expression patterns at various tissue sites, abiotic stress and hormone induction. Because FmSPL2 is highly expressed in flowers it was selected to describe the SPL gene family of Fraxinus mandshurica by ectopic expression. Among them, 10 FmSPL genes that were highly expressed at different loci were selected for expression analysis under abiotic stress (NaCl and Cold) and hormone induction (IAA and ABA). These 10 FmSPL genes showed corresponding trends in response to both abiotic stress and hormone induction. We showed that overexpression of FmSPL2 in transgenic Nicotiana tabacum L. resulted in taller plants, shorter root length, increased root number, rounded leaves, and earlier flowering time. Conclusions We identified 36 SPL genes, which were classified into seven subfamilies based on sequence analysis. FmSPL2 was selected for subsequent heterologous expression by analysis of expression patterns in various tissues and under abiotic stress and hormone induction, and significant phenotypic changes were observed in the transgenic Nicotiana tabacum L. These results provide insight into the evolutionary origin and biological significance of plant SPL. The aim of this study was to lay the foundation for the genetic improvement of Fraxinus mandshurica and the subsequent functional analysis of FmSPL2.

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Glucose- and sucrose-signaling modules regulate the Arabidopsis juvenile-to-adult phase transition

Abstract: Highlights d CINV1/2 are key endogenous factors that control the Arabidopsis juvenile-to-adult transition d A glucose-signaling feed-forward loop dynamically controls the juvenile-to-adult transition d A sucrose-signaling pathway regulates the juvenile-to-adult transition

Cited by 33 publications

References 50 publications

Feedback loop promotes sucrose accumulation in cotyledons to facilitate sugar-ethylene signaling-mediated, etiolated-seedling greening

Feedback loop promotes sucrose accumulation in cotyledons to facilitate sugar-ethylene signaling-mediated, etiolated-seedling greening

Two AT‐Hook proteins regulate A/NINV7 expression to modulate sucrose catabolism for cold tolerance in Poncirus trifoliata

Genome-wide analysis and molecular dissection of the SPL gene family in Fraxinus mandshurica

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