Glucose Enhances Endothelial LOX-1 Expression

Abstract

Endothelial dysfunction is an early and key determinant of diabetic vascular complications that is elicited at least in part by oxidized LDL (oxLDL). The recent observation that lectin-like oxLDL receptor-1 (LOX-1) expression is increased in the vascular endothelium of diabetic rats suggests a role for LOX-1 in the pathogenesis of diabetic vascular dysfunction. Because postprandial plasma glucose has been recently proposed as an independent risk factor for cardiovascular diseases in patients with diabetes, we evaluated, in the current study, the in vitro effect of high glucose on LOX-1 expression by human aortic endothelial cells (

Soluble lectin-like oxidized low density lipoprotein receptor-1 in type 2 diabetes mellitus

“…We report for the first time that the serum sLOX-1 level is elevated in patients with type 2 diabetes mellitus and that there is a correlation between AGEs and sLOX-1. This is in keeping with the findings from both in vitro and animal studies showing that LOX-1 expression is increased by the hyperglycemic milieu (12,13,15). Moreover, we have confirmed that both glucose and AGEs can increase the level of sLOX-1 in conditioned medium of cultured endothelial cells.…”

“…LOX-1 is a receptor with an expression that is not constitutive but dynamically inducible (10), and LOX-1 is upregulated by a number of pathophysiological stimuli, including oxLDL, shear stress, inflammatory cytokines, and angiotensin II (3,4,11). In addition, it has been reported that LOX-1 expression can be increased by glucose both in macrophages and in endothelial cells (12,13). LOX-1 is an endothelial receptor for advanced glycation end products (AGEs) (14), and AGEs can induce LOX-1 expression in cultured endothelial cells and macrophages (15,16).…”

Soluble Lectin-Like Oxidized LDL Receptor-1 and High-Sensitivity Troponin T as Diagnostic Biomarkers for Acute Coronary Syndrome - Improved Values With Combination Usage in Emergency Rooms -

“…In addition, differences in plaque characteristics, such as rupture or erosion, may also have affected the difference in sLOX-1 levels between the STEACS and NSTEACS groups. Plasma sLOX-1 levels were higher in patients with diabetes and a smoking habit than in those without, probably because high glucose levels induce the expression of LOX-1, as previously shown by an in vitro study, 26 and smoking affects plaque vulnerability by inducing oxidative stress and inflammation. 34 Serum peak CK-MB values were higher in patients with a sLOX-1 value ≥131.7 pg/ml in the ER than in those with a value <131.7 pg/ml, which means the sLOX-1 values in the ER probably indicated the severity of ACS.…”

Modulation of placental protein expression of OLR1: implication in pregnancy-related disorders or pathologies

“…This result, for the protein expression, is in accordance with other studies that demonstrated the increase in OLR1 expression in different tissues of diabetic subjects. It has been reported that OLR1 expression is increased by glucose both in macrophage and endothelial cells (Li et al 2003(Li et al , 2004. Effectively, diabetes is characterized by the presence of an important oxidative stress, endothelial dysfunction, and increased expression of endothelial molecules in the inflammatory cells (Stevens 2005).…”