Glucose signalling positively regulates aliphatic glucosinolate biosynthesis

Miao, Heng; Jia, Wei; Zhao, Yong; Yan, Huimin; Sun, Bo; Huang, Jirong; Wang, Qiaomei

doi:10.1093/jxb/ers399

Cited by 81 publications

(62 citation statements)

References 54 publications

(92 reference statements)

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“…It involves the integration of signals from sugars (Miao et al, 2013), brassinosteroids (Guo et al, 2013a), jasmonic acid (Guo et al, 2013b), salicylic acid (Mewis et al, 2005), ethylene, and auxin (Schweizer et al, 2013), in addition to a network of TFs (Gigolashvili et al, 2007;Malitsky et al, 2008;Sønderby et al, 2010). Our analysis has identified several TF as potential regulators of GS biosynthesis in the Arabidopsis funiculus.…”

Section: The Funiculus As a Center For Cellular Bioenergetics And Fatmentioning

confidence: 90%

Transcriptome atlas of the Arabidopsis funiculus – a study of maternal seed subregions

et al. 2015

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These authors contributed equally to this manuscript. SUMMARYThe funiculus anchors the structurally complex seed to the maternal plant, and is the only direct route of transport for nutrients and maternal signals to the seed. While our understanding of seed development is becoming clearer, current understanding of the genetics and cellular mechanisms that contribute to funiculus development is limited. Using laser microdissection combined with global RNA-profiling experiments we compared the genetic profiles of all maternal and zygotic regions and subregions during seed development. We found that the funiculus is a dynamic region of the seed that is enriched for mRNAs associated with hormone metabolism, molecular transport, and metabolic activities corresponding to biological processes that have yet to be described in this maternal seed structure. We complemented our genetic data with a complete histological analysis of the funiculus from the earliest stages of development through to seed maturation at the light and electron microscopy levels. The anatomy revealed signs of photosynthesis, the endomembrane system, cellular respiration, and transport within the funiculus, all of which supported data from the transcriptional analysis. Finally, we studied the transcriptional programming of the funiculus compared to other seed subregions throughout seed development. Using newly designed in silico algorithms, we identified a number of transcriptional networks hypothesized to be responsible for biological processes like auxin response and glucosinolate biosynthesis found specifically within the funiculus. Taken together, patterns of gene activity and histological observations reveal putative functions of the understudied funiculus region and identify predictive transcriptional circuits underlying these biological processes in space and time.

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Section: The Funiculus As a Center For Cellular Bioenergetics And Fatmentioning

confidence: 90%

Transcriptome atlas of the Arabidopsis funiculus – a study of maternal seed subregions

et al. 2015

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“…GSs were extracted and analyzed as previously described (Miao et al, 2013). Twelve-day-old seedlings were harvested at indicated times after treatment.…”

Section: Hplc Assay Of Glucosinolatesmentioning

confidence: 99%

Pathogen-Responsive MPK3 and MPK6 Reprogram the Biosynthesis of Indole Glucosinolates and Their Derivatives in Arabidopsis Immunity

et al. 2016

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ORCID IDs: 0000-0001-6683-6415 (J.X.); 0000-0002-9910-3119 (J.M.); 0000-0002-4265-7399 (J.L.); 0000-0002-0757-5208 (Q.W.); 0000-0003-2959-6461 (S.Z.)Antimicrobial compounds have critical roles in plant immunity; for example, Arabidopsis thaliana and other crucifers deploy phytoalexins and glucosinolate derivatives in defense against pathogens. The pathogen-responsive MITOGEN-ACTIVATED PROTEIN KINASE3 (MPK3) and MPK6 have essential functions in the induction of camalexin, the major phytoalexin in Arabidopsis. In search of cyanide, a coproduct of ethylene and camalexin biosynthesis, we found that MPK3 and MPK6 also affect the accumulation of extracellular thiocyanate ion derived from the indole glucosinolate (IGS) pathway. Botrytis cinerea infection activates MPK3/MPK6, which promote indole-3-yl-methylglucosinolate (I3G) biosynthesis and its conversion to 4-methoxyindole-3-yl-methylglucosinolate (4MI3G). Gain-and loss-of-function analyses demonstrated that MPK3/MPK6 regulate the expression of MYB51 and MYB122, two key regulators of IGS biosynthesis, as well as CYP81F2 and IGMT1/ IGMT2, which encode enzymes in the conversion of I3G to 4MI3G, through ETHYLENE RESPONSE FACTOR6 (ERF6), a substrate of MPK3/MPK6. Under the action of PENETRATION2 (PEN2), an atypical myrosinase, and PEN3, an ATP binding cassette transporter, 4MI3G is converted to extracellular unstable antimicrobial compounds, possibly isothiocyanates that can react with nucleophiles and release the stable thiocyanate ion. Recent studies demonstrated the importance of PEN2/ PEN3-dependent IGS derivatives in plant immunity. Here, we report that MPK3/MPK6 and their substrate ERF6 promote the biosynthesis of IGSs and the conversion of I3G to 4MI3G, a target of PEN2/PEN3-dependent chemical defenses in plant immunity.

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“…The glucosinolate content varies depending on genetic and environmental factors, and is modulated by external signals such as sugar (Miao et al, 2013) and phytohormones (Yuan et al, 2009;Sun et al, 2012;Wang et al, 2012;Guo et al, 2013a;2013b;Huseby et al, 2013;Kim et al, 2013;Zang et al, 2015). Glucose is not only the basal carbon and energy source but also a signaling molecule having regulatory effects throughout the life cycle of plant (Sheen, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Glucose is not only the basal carbon and energy source but also a signaling molecule having regulatory effects throughout the life cycle of plant (Sheen, 2014). Our previous studies showed that glucose dramatically increases glucosinolate content in both Arabidopsis and crop brassicas (Wei et al, 2011;Guo et al, 2013c;Miao et al, 2013). Gibberellins are plant hormones participating in the regulation of plant development, which are used extensively to elevate crop yield (Naeem et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Effects of glucose and gibberellic acid on glucosinolate content and antioxidant properties of Chinese kale sprouts

Miao

Wang

Chang

et al. 2017

J. Zhejiang Univ. Sci. B

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Glucosinolates, anthocyanins, total phenols, and vitamin C, as well as antioxidant capacity, were investigated in Chinese kale sprouts treated with both glucose and gibberellic acid (GA 3 ). The combination of 3% (0.03 g/ml) glucose and 5 μmol/L GA 3 treatment was effective in increasing glucosinolate content while glucose or GA 3 treatment alone did not influence significantly almost all individual glucosinolates or total glucosinolates. The total phenolic content and antioxidant activity of Chinese kale sprouts were enhanced by combined treatment with glucose and GA 3 , which could be useful in improving the main health-promoting compounds and antioxidant activity in Chinese kale sprouts.

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Glucose signalling positively regulates aliphatic glucosinolate biosynthesis

Cited by 81 publications

References 54 publications

Transcriptome atlas of the Arabidopsis funiculus – a study of maternal seed subregions

Transcriptome atlas of the Arabidopsis funiculus – a study of maternal seed subregions

Pathogen-Responsive MPK3 and MPK6 Reprogram the Biosynthesis of Indole Glucosinolates and Their Derivatives in Arabidopsis Immunity

Effects of glucose and gibberellic acid on glucosinolate content and antioxidant properties of Chinese kale sprouts

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