GLUT4 translocation in C2C12 myoblasts and primary mouse hepatocytes by an antihyperglycemic flavone from Tillandsia usneoides

Miranda-Nuñez, Jhovan Eduardo; Zamilpa-Alvarez, Alejandro; Fortis-Barrera, Ángeles; Alarcón-Aguilar, Francisco Javier; Loza-Rodríguez, Hilda; Gómez–Quiroz, Luis E.; Salas-Silva, Soraya; Flores-Cruz, María; Zavala-Sánchez, Miguel Ángel; Blancas-Flores, Gerardo

doi:10.1016/j.phymed.2021.153622

Cited by 9 publications

(3 citation statements)

References 38 publications

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“…The free fatty acid was prepared using sodium palmitate conjugated with fatty-acid-free BSA (bovine serum albumin). 25 After the treatment, the 96-well plate was incubated in a 5% CO 2 incubator at 37 °C for 8 h. Insulin (100 nM) was added as a positive control for an incubation period of 30 min. After that, the cells were exposed to 2-NBDG (2-[ N -(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose) at a dosage of 100 μL per well and incubated in a 5% CO 2 incubator at 37 °C for 30 min 2-NBDG is a fluorescent glucose moiety prepared by dilution to 80 μM with a glucose-free, serum-free medium.…”

Section: Methodsmentioning

confidence: 99%

Chemical composite of indigenous whole grain scented joha rice varietal prevents type 2 diabetes in rats through ameliorating insulin sensitization by the IRS-1/AKT/PI3K signalling cascade

et al. 2022

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Section: Methodsmentioning

confidence: 99%

Chemical composite of indigenous whole grain scented joha rice varietal prevents type 2 diabetes in rats through ameliorating insulin sensitization by the IRS-1/AKT/PI3K signalling cascade

et al. 2022

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“…It has traditionally been used in the control of diabetes [ 8 ] and its activity is attributed to the presence of 3-hydroxy-3-methylglutaric acid (HMG), a highly toxic compound [ 9 ]. However, other compounds with hypoglycemic activity, possibly exerted through GLUT4 modulation and with low cellular toxicity, have been identified, such as 5,7,4′-trihydroxy-3,6,3′,5′-tetramethoxyflavone (Flav1) [ 10 ]. Although antitumor effects for Tillandsia recurvata were reported in 2007 [ 11 ], not much progress has been made in understanding the mechanisms involved in this activity.…”

Section: Introductionmentioning

confidence: 99%

Tillandsia usneoides Extract Decreases the Primary Tumor in a Murine Breast Cancer Model but Not in Melanoma

Lasso

Rojas

Arévalo

et al. 2022

Cancers

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The main limits of current antitumor therapies are chemoresistance, relapses, and toxicity that impair patient quality of life. Therefore, the discovery of therapeutic alternatives, such as adjuvants to conventional therapy that modulate the intracellular oxidation state or the immune response, remains a challenge. Owing to traditional medicine, several uses of plants are known, indicating a promising antitumor and immunomodulatory effect. We evaluated the effect of ethanolic extract of T. usneoides in vitro and in vivo in models of 4T1 breast cancer and B16-F10 melanoma. In vitro evaluations with both cell lines showed that the extract has cytotoxic activity and induces apoptotic cell death. However, its effect on ROS production and glucose uptake was opposite. In vivo, only in the 4T1 model, a significant decrease in tumor size was found in animals treated with the extract, accompanied by an increase in dendritic cells and activated CD8+ T cells, and a decrease in myeloid-derived suppressor-like cells (MDSC-LC) and Tregs in the tumor microenvironment. These results suggest that T. usneoides extract antagonistically regulates tumor metabolism of 4T1 vs. B16-F10, impacting the tumor microenvironment and effective antitumor immune response, leading to a reduction in 4T1 tumor size but not on B16-F10.

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“…TU-AcOEt and TU-Aqueous significantly reduced blood glucose by 240 (21 and 27%, respectively) and 360 min (21 and 38%, respectively) compared to the control group. The flavonoid (5,7,4'trihydroxy-3,6,3',5'-tetramethoxyflavone) exhibited an antihyperglycemic effect mediated by GLUT4 translocation in muscles and hepatocytes(Miranda-Nuñez et al, 2021).…”

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Investigations on the chemical and bioactivity profile of Bromeliaceae Juss

Furtado,

Rodrigues,

Vasconcelos

et al. 2024

OLEL

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Bromeliaceae Juss., in several cultures for thousands of years it has been used for food, fibers, in ceremonies, medicines and ornamental plants. The phytochemical composition of the species of the family is chemodiverse, with the presence of fatty acids, phenolic compounds, vitamins, triterpenes and enzymes, exerting antibacterial, antimicrobial, hypoglycemic, antileukemic, proteolytic activities, non-polar natural products and production of bioactive peptides. As a result, the family has great chemical and pharmacological potential in order to contribute to scientific knowledge and the development of new drugs.

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GLUT4 translocation in C2C12 myoblasts and primary mouse hepatocytes by an antihyperglycemic flavone from Tillandsia usneoides

Cited by 9 publications

References 38 publications

Chemical composite of indigenous whole grain scented joha rice varietal prevents type 2 diabetes in rats through ameliorating insulin sensitization by the IRS-1/AKT/PI3K signalling cascade

Chemical composite of indigenous whole grain scented joha rice varietal prevents type 2 diabetes in rats through ameliorating insulin sensitization by the IRS-1/AKT/PI3K signalling cascade

Tillandsia usneoides Extract Decreases the Primary Tumor in a Murine Breast Cancer Model but Not in Melanoma

Investigations on the chemical and bioactivity profile of Bromeliaceae Juss

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