Glutamate and histidine improve both solvent yields and the acid tolerance response of <i>Clostridium beijerinckii</i>
 NCP 260

Reeve, Byron W P; Reid, Sharon J.

doi:10.1111/jam.13067

Cited by 19 publications

(8 citation statements)

References 34 publications

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“…Additionally, the contribution of glutamine to acid tolerance via the release of ammonia mediated by glutaminase was previously investigated (Lu et al, ). Aspartate and histidine were also found to be involved in the acid tolerance response of bacteria (Reeve & Reid, ; Wu, Zhang, Du, & Chen, ).…”

Section: Discussionmentioning

confidence: 99%

Comparative genomics and transcriptomics analysis‐guided metabolic engineering of Propionibacterium acidipropionici for improved propionic acid production

Guan

et al. 2017

Biotech & Bioengineering

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Acid stress induced by the accumulation of organic acids during the fermentation of propionibacteria is a severe limitation in the microbial production of propionic acid (PA). To enhance the acid resistance of strains, the tolerance mechanisms of cells must first be understood. In this study, comparative genomic and transcriptomic analyses were conducted on wild-type and acid-tolerant Propionibacterium acidipropionici to reveal the microbial response of cells to acid stress during fermentation. Combined with the results of previous proteomic and metabolomic studies, several potential acid-resistance mechanisms of P. acidipropionici were analyzed. Energy metabolism and transporter activity of cells were regulated to maintain pH homeostasis by balancing transmembrane transport of protons and ions; redundant protons were eliminated by enhancing the metabolism of certain amino acids for a relatively stable intracellular microenvironment; and protective mechanism of macromolecules were also induced to repair damage to proteins and DNA by acids. Transcriptomic data indicated that the synthesis of acetate and lactate were undesirable in the acid-resistant mutant, the expression of which was 2.21-fold downregulated. In addition, metabolomic data suggested that the accumulation of lactic acid and acetic acid reduced the carbon flow to PA and led to a decrease in pH. On this basis, we propose a metabolic engineering strategy to regulate the synthesis of lactic acid and acetic acid that will reduce by-products significantly and increase the PA yield by 12.2% to 10.31 ± 0.84 g/g DCW. Results of this study provide valuable guidance to understand the response of bacteria to acid stress and to construct microbial cell factories to produce organic acids by combining systems biology technologies with synthetic biology tools.

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Section: Discussionmentioning

confidence: 99%

Comparative genomics and transcriptomics analysis‐guided metabolic engineering of Propionibacterium acidipropionici for improved propionic acid production

Guan

et al. 2017

Biotech & Bioengineering

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“…2). Glutamate decarboxylase catalyzes the decarboxylation of glutamate, yielding γ-aminobutyric acid (GABA) and carbon dioxide, accompanied by proton consumption (Reeve and Reid 2016). The specific amino acid antiporter GadC, which is also known to transport glutamine, transports extracellular glutamate and intracellular GABA (Laroute et al 2016;Ma et al 2012).…”

Section: Consumption Of Protonsmentioning

confidence: 99%

Microbial response to acid stress: mechanisms and applications

Guan

Liu

2019

Appl Microbiol Biotechnol

394

194

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Microorganisms encounter acid stress during multiple bioprocesses. Microbial species have therefore developed a variety of resistance mechanisms. The damage caused by acidic environments is mitigated through the maintenance of pH homeostasis, cell membrane integrity and fluidity, metabolic regulation, and macromolecule repair. The acid tolerance mechanisms can be used to protect probiotics against gastric acids during the process of food intake, and can enhance the biosynthesis of organic acids. The combination of systems and synthetic biology technologies offers new and wide prospects for the industrial applications of microbial acid tolerance mechanisms. In this review, we summarize acid stress response mechanisms of microbial cells, illustrate the application of microbial acid tolerance in industry, and prospect the introduction of systems and synthetic biology to further explore the acid tolerance mechanisms and construct a microbial cell factory for valuable chemicals.

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“…Acids, various metals and minerals, vitamins, and amino acids also affect both solvent production and sporulation in clostridia (List et al 2019;Mukherjee et al 2019;Nimbalkar et al 2018Nimbalkar et al , 2019Reeve and Reid 2016), but few studies mentioned their impact on sporulation. Long et al (1984a) investigated butyrate and acetate's effect on sporulation in C. saccharobutylicum by adding them to the medium at the start of the fermentation in different concentrations.…”

Section: Other Media Componentsmentioning

confidence: 99%

Sporulation in solventogenic and acetogenic clostridia

Diallo

Kengen

López-Contreras

2021

Appl Microbiol Biotechnol

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The Clostridium genus harbors compelling organisms for biotechnological production processes; while acetogenic clostridia can fix C1-compounds to produce acetate and ethanol, solventogenic clostridia can utilize a wide range of carbon sources to produce commercially valuable carboxylic acids, alcohols, and ketones by fermentation. Despite their potential, the conversion by these bacteria of carbohydrates or C1 compounds to alcohols is not cost-effective enough to result in economically viable processes. Engineering solventogenic clostridia by impairing sporulation is one of the investigated approaches to improve solvent productivity. Sporulation is a cell differentiation process triggered in bacteria in response to exposure to environmental stressors. The generated spores are metabolically inactive but resistant to harsh conditions (UV, chemicals, heat, oxygen). In Firmicutes, sporulation has been mainly studied in bacilli and pathogenic clostridia, and our knowledge of sporulation in solvent-producing or acetogenic clostridia is limited. Still, sporulation is an integral part of the cellular physiology of clostridia; thus, understanding the regulation of sporulation and its connection to solvent production may give clues to improve the performance of solventogenic clostridia. This review aims to provide an overview of the triggers, characteristics, and regulatory mechanism of sporulation in solventogenic clostridia. Those are further compared to the current knowledge on sporulation in the industrially relevant acetogenic clostridia. Finally, the potential applications of spores for process improvement are discussed.Key Points• The regulatory network governing sporulation initiation varies in solventogenic clostridia.• Media composition and cell density are the main triggers of sporulation.• Spores can be used to improve the fermentation process.

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Glutamate and histidine improve both solvent yields and the acid tolerance response of Clostridium beijerinckii NCP 260

Abstract: Using substrates naturally rich in glutamine, glutamate and histidine in industrial fermentations is a promising means to increase acid survival and solvent yields in solventogenic Clostridium.

Cited by 19 publications

References 34 publications

Comparative genomics and transcriptomics analysis‐guided metabolic engineering of Propionibacterium acidipropionici for improved propionic acid production

Comparative genomics and transcriptomics analysis‐guided metabolic engineering of Propionibacterium acidipropionici for improved propionic acid production

Microbial response to acid stress: mechanisms and applications

Sporulation in solventogenic and acetogenic clostridia

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