Glutamic acid decarboxylase 65, 67, and GABA-transaminase mRNA expression and total enzyme activity in the goldfish (Carassius auratus) brain

Martyniuk, Christopher J.; Awad, Rosalie; Hurley, Rachel M.; Finger, Thomas E.; Trudeau, Vance L.

doi:10.1016/j.brainres.2007.02.010

Cited by 34 publications

(25 citation statements)

References 47 publications

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“…In the granule cell layer, the isolated cell bodies immunolabeled for GAD65 and GABA in this study appear to be Golgi cells. These cells are known to be GABAergic in mammals, and have recently been shown to express GAD65 and GAD67 mRNA in the goldfish [22], which supports this interpretation.…”

Section: Discussionsupporting

confidence: 60%

“…In the goldfish, susceptibility to kainic acid suggested that Purkinje, stellate, and Golgi cells are GABAergic [21]. A recent in situ hybridization study also concluded that goldfish Purkinje and Golgi cells are GABAergic [22]. In the salmon Salmo salar, the β2/β3 subunits of the GABA A receptor localize to putative Golgi cells in the granular layer and to some processes in the molecular layer, but were not detected in the cell bodies of Purkinje cells [23].…”

Section: Discussionmentioning

confidence: 99%

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Immunohistochemical Localization of GABA, GAD65, and the Receptor Subunits GABAAα1 and GABAB1 in the Zebrafish Cerebellum

Delgado

Schmachtenberg

2008

Cerebellum

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Gamma-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter in the central nervous system. Its role is especially prominent in the cerebellum, where most neuron types are GABAergic. However, little is known about its function in the cerebellum of teleost fish, which is only partly homologous to its mammalian counterpart. Here, we investigated the expression and distribution of GABA, the GABA-synthesizing enzyme glutamic acid decarboxylase 65 (GAD65), and the receptor subunits GABA(Aalpha1) and GABA(B1) in the cerebellum of adult zebrafish. GABA and GAD65 presented a similar expression pattern that comprised the molecular layer, Purkinje cells and groups of presumed Golgi cells in the granular layer, both in the cerebellar corpus and valve. GABA(A) receptor subunits are principally found on fine radial fibers in the molecular layer, while GABA(B) receptor subunits localized prominently to the cell bodies of Purkinje cells in the ganglionic layer, and to their dendrites that span the molecular layer. These results are compared to the expression of the GABAergic system in the mammalian cerebellum.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Localization of GABA, GAD65, and the Receptor Subunits GABAAα1 and GABAB1 in the Zebrafish Cerebellum

Delgado

Schmachtenberg

2008

Cerebellum

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“…GABA is a major inhibitory neurotransmitter in both vertebrates and invertebrates [43-46]. In comparison to other animals, the distribution of GABA in the CNS of amphioxus embryos and larvae seems to be more discrete.…”

Section: Discussionmentioning

confidence: 99%

A neurochemical map of the developing amphioxus nervous system

et al. 2012

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BackgroundAmphioxus, representing the most basal group of living chordates, is the best available proxy for the last invertebrate ancestor of the chordates. Although the central nervous system (CNS) of amphioxus comprises only about 20,000 neurons (as compared to billions in vertebrates), the developmental genetics and neuroanatomy of amphioxus are strikingly vertebrate-like. In the present study, we mapped the distribution of amphioxus CNS cells producing distinctive neurochemicals. To this end, we cloned genes encoding biosynthetic enzymes and/or transporters of the most common neurotransmitters and assayed their developmental expression in the embryo and early larva.ResultsBy single and double in situ hybridization experiments, we identified glutamatergic, GABAergic/glycinergic, serotonergic and cholinergic neurons in developing amphioxus. In addition to characterizing the distribution of excitatory and inhibitory neurons in the developing amphioxus CNS, we observed that cholinergic and GABAergic/glycinergic neurons are segmentally arranged in the hindbrain, whereas serotonergic, glutamatergic and dopaminergic neurons are restricted to specific regions of the cerebral vesicle and the hindbrain. We were further able to identify discrete groups of GABAergic and glutamatergic interneurons and cholinergic motoneurons at the level of the primary motor center (PMC), the major integrative center of sensory and motor stimuli of the amphioxus nerve cord.ConclusionsIn this study, we assessed neuronal differentiation in the developing amphioxus nervous system and compiled the first neurochemical map of the amphioxus CNS. This map is a first step towards a full characterization of the neurotransmitter signature of previously described nerve cell types in the amphioxus CNS, such as motoneurons and interneurons.

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“…However, this SN-innervation surrounds the caudalmost part of the NRL. In situ hybridization studies indicate high expression of GAD65 and to a lesser extent GAD67 in neurons lining the goldfish ventrocaudal NRL (Martyniuk et al, 2007). Therefore, it is possible that SN-ir terminals are innervating these GABAergic neurons.…”

Section: Diencephalonmentioning

confidence: 99%

Forebrain mapping of secretoneurin‐like immunoreactivity and its colocalization with isotocin in the preoptic nucleus and pituitary gland of goldfish

Canosa¹,

López²,

Scharrig³

et al. 2011

J of Comparative Neurology

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Secretoneurin, a 33-34 amino acid neuropeptide derived from the proteolytic processing of the secretogranin-II precursor protein, is reasonably well conserved in evolution. Goldfish secretoneurin shares >75% similarity overall with other vertebrate secretoneurin sequences. The secretoneurin peptide has numerous functions that include neuroinflammation, neurotransmitter release, and neuroendocrine regulation. A detailed description of the central distribution of secretoneurin immunoreactivity is only known for the rat. Using our polyclonal antibody against the central, conserved core of the secretoneurin peptide we studied the distribution of secretoneurin-like immunoreactivity in the goldfish brain. Secretoneurin immunoreactivity was found in the olfactory bulb, entopeduncular nucleus, preoptic nucleus, lateral part of the lateral tuberal nucleus, posterior periventricular nucleus, nucleus of the posterior recess, the nucleus of the saccus vasculosus, and nucleus isthmi. Secretoneurin-immunoreactive fibers were found in the dorsal part of the dorsal telencephalon, ventral and lateral parts of the ventral telencephalon, periventricular preoptic nucleus, pituitary, and the ventrocaudal aspect of the nucleus of the lateral recess. The most conspicuous secretoneurin immunoreactivity was found in the magnocellular and parvocellular cells of the preoptic nucleus that project to the pituitary. Double-labeling studies indicated coexpression with isotocin, the fish homolog of mammalian oxytocin. Clear colabeling for secretoneurin and isotocin in fibers terminating in the neurointermediate lobe suggests that secretoneurin maybe coreleased with isotocin. Previous work indicates that secretoneurin stimulates the release of luteinizing hormone from the goldfish anterior pituitary. Our findings further support a reproductive role for secretoneurin and related peptides, given the importance of oxytocin family peptides in reproductive behavior in vertebrates.

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Glutamic acid decarboxylase 65, 67, and GABA-transaminase mRNA expression and total enzyme activity in the goldfish (Carassius auratus) brain

Cited by 34 publications

References 47 publications

Immunohistochemical Localization of GABA, GAD65, and the Receptor Subunits GABAAα1 and GABAB1 in the Zebrafish Cerebellum

Immunohistochemical Localization of GABA, GAD65, and the Receptor Subunits GABAAα1 and GABAB1 in the Zebrafish Cerebellum

A neurochemical map of the developing amphioxus nervous system

Forebrain mapping of secretoneurin‐like immunoreactivity and its colocalization with isotocin in the preoptic nucleus and pituitary gland of goldfish

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