Glutamic Acid Signal Synchronizes Protein Synthesis Kinetics in Hepatocytes from Old Rats for the Following Several Days. Cell Metabolism Memory

Brodsky, V. Ya.; Мальченко, Л. А.; Lazarev, Denis; Буторина, Н. Н.; Dubovaya, T. K.; Zvezdina, N. D.

doi:10.1134/s0006297918030094

Cited by 5 publications

(5 citation statements)

References 14 publications

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“…Goodwin, 130,131 Gilbert, 132,133 Hildebrandt, 134 and Yates [135][136][137][138] repeatedly emphasized these principles in seminal publications. Brodsky and his coworkers 139,140 continue with key physiological experiments in his 90th year.…”

Section: Further Analytical Refinements At the University Of Vienna Amentioning

confidence: 99%

Temporal metabolic partitioning of the yeast and protist cellular networks: the cell is a global scale-invariant (fractal or self-similar) multioscillator

et al. 2018

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Britton Chance, electronics expert when a teenager, became an enthusiastic student of biological oscillations, passing on this enthusiasm to many students and colleagues, including one of us (DL). This historical essay traces BC's influence through the accumulated work of DL to DL's many collaborators. The overall temporal organization of mass-energy, information, and signaling networks in yeast in self-synchronized continuous cultures represents, until now, the most characterized example of in vivo elucidation of time structure. Continuous online monitoring of dissolved gases by direct measurement (membrane-inlet mass spectrometry, together with NAD(P)H and flavin fluorescence) gives strain-specific dynamic information from timescales of minutes to hours as does two-photon imaging. The predominantly oscillatory behavior of network components becomes evident, with spontaneously synchronized cellular respiration cycles between discrete periods of increased oxygen consumption (oxidative phase) and decreased oxygen consumption (reductive phase). This temperature-compensated ultradian clock provides coordination, linking temporally partitioned functions by direct feedback loops between the energetic and redox state of the cell and its growing ultrastructure. Multioscillatory outputs in dissolved gases with 13 h, 40 min, and 4 min periods gave statistical self-similarity in power spectral and relative dispersional analyses: i.e., complex nonlinear (chaotic) behavior and a functional scale-free (fractal) network operating simultaneously over several timescales.

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Section: Further Analytical Refinements At the University Of Vienna Amentioning

confidence: 99%

Temporal metabolic partitioning of the yeast and protist cellular networks: the cell is a global scale-invariant (fractal or self-similar) multioscillator

et al. 2018

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“…Furthermore, both glutamine and glutamic acid are integral components of the TCA cycle and are essential constituents of the antioxidant glutathione (Tompkins et al, 2019). It has shown that supplementation with glutamic acid significantly increases protein synthesis in primary cultures of hepatocytes from elderly rats, effectively doubling the rate in comparison to elderly rats not receiving glutamic acid supplementation (Brodsky et al, 2018). Additionally, glutathione, a well-known antioxidant, plays a crucial role in combating free radicals to mitigate oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Fluoride impairs vascular smooth muscle cells via disrupting amino acids metabolism

Li,

Yang,

et al. 2024

Preprint

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Given the insidious and high-fatality nature of cardiovascular diseases (CVDs), the emergence of fluoride as a newly identified risk factor demands serious consideration alongside traditional risk factors. While vascular smooth muscle cells (VSMCs) play a pivotal role in the progression of CVDs, the toxicological impact of fluoride on VSMCs remains largely uncharted. In this study, fluoride aggravated the pathological damage of rat aorta in vivo. Then rat VSMC lines (A7R5) were exposed to fluoride with concentration ranging from 0 to 1200 μmol/L over a 24-hour period, revealing a dose-dependent inhibition of cell proliferation and migration. The further metabolomic analysis showed alterations in metabolite profiles induced by fluoride exposure, notably decreasing organic acids and lipid molecules level. Additionally, gene network analysis underscored the frequency of fluoride's interference with amino acids metabolism, potentially impacting the tricarboxylic acid (TCA) cycle. Our results also highlighted the ATP-binding cassette (ABC) transporters pathway as a central element in VSMC impairment. Moreover, we observed a dose-dependent increase in osteopontin (OPN) and α-smooth muscle actin (α-SMA) mRNA level and a dose-dependent decrease in ABC subfamily C member 1 (ABCC1) and bestrophin 1 (BEST1) mRNA level. These findings advance our understanding of fluoride as a CVD risk factor and its influence on VSMCs and metabolic pathways, warranting further investigation into this emerging risk factor.

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“…Furthermore, glutamine and glutamate participate in the TCA cycle in many forms and are essential constituents of the antioxidant glutathione [42]. It has shown that supplementation with glutamic acid significantly increases protein synthesis in primary cultures of hepatocytes from elderly rats, effectively doubling the rate in comparison to elderly rats not receiving glutamic acid supplementation [4]. Additionally, glutathione, a well-known antioxidant, plays a crucial role in combating free radicals to mitigate oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Fluoride impairs vascular smooth muscle A7R5 cell lines via disrupting amino acids metabolism

Li,

Yang,

et al. 2024

J Transl Med

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Given the insidious and high-fatality nature of cardiovascular diseases (CVDs), the emergence of fluoride as a newly identified risk factor demands serious consideration alongside traditional risk factors. While vascular smooth muscle cells (VSMCs) play a pivotal role in the progression of CVDs, the toxicological impact of fluoride on VSMCs remains largely uncharted. In this study, we constructed fluorosis model in SD rats and A7R5 aortic smooth muscle cell lines to confirm fluoride impaired VSMCs. Fluoride aggravated the pathological damage of rat aorta in vivo. Then A7R5 were exposed to fluoride with concentration ranging from 0 to 1200 μmol/L over a 24-h period, revealing a dose-dependent inhibition of cell proliferation and migration. The further metabolomic analysis showed alterations in metabolite profiles induced by fluoride exposure, notably decreasing organic acids and lipid molecules level. Additionally, gene network analysis underscored the frequency of fluoride's interference with amino acids metabolism, potentially impacting the tricarboxylic acid (TCA) cycle. Our results also highlighted the ATP-binding cassette (ABC) transporters pathway as a central element in VSMC impairment. Moreover, we observed a dose-dependent increase in osteopontin (OPN) and α-smooth muscle actin (α-SMA) mRNA level and a dose-dependent decrease in ABC subfamily C member 1 (ABCC1) and bestrophin 1 (BEST1) mRNA level. These findings advance our understanding of fluoride as a CVD risk factor and its influence on VSMCs and metabolic pathways, warranting further investigation into this emerging risk factor.

show abstract

Glutamic Acid Signal Synchronizes Protein Synthesis Kinetics in Hepatocytes from Old Rats for the Following Several Days. Cell Metabolism Memory

Cited by 5 publications

References 14 publications

Temporal metabolic partitioning of the yeast and protist cellular networks: the cell is a global scale-invariant (fractal or self-similar) multioscillator

Temporal metabolic partitioning of the yeast and protist cellular networks: the cell is a global scale-invariant (fractal or self-similar) multioscillator

Fluoride impairs vascular smooth muscle cells via disrupting amino acids metabolism

Fluoride impairs vascular smooth muscle A7R5 cell lines via disrupting amino acids metabolism

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