Glutamine and alanine-induced differential expression of intracellular IL-6, IL-8, and TNF-α in LPS-stimulated monocytes in human whole-blood

Raspé, Christoph; Czeslick, Elke; Weimann, Arved; Schinke, Carolina; Leimert, Anja; Kellner, Patrick; Simm, Andreas; Bucher, Michael; Sablotzki, Armin

doi:10.1016/j.cyto.2013.02.020

Cited by 26 publications

(21 citation statements)

References 25 publications

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“…Of interest is the observation that CIT reduced IL‐6 and TNF‐α mRNA expressions in explants from HFD rats, a situation at variance with that in old rats. This observation is in accordance with several studies previously demonstrating that amino acids and derivatives decrease proinflammatory markers in mouse or human adipocytes or monocytes . As expected from previous work, HFD induces Nos2 expression .…”

Section: Discussionsupporting

confidence: 94%

Citrulline reduces glyceroneogenesis and induces fatty acid release in visceral adipose tissue from overweight rats

Joffin

Jaubert

Durant

et al. 2014

Molecular Nutrition Food Res

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Section: Discussionsupporting

confidence: 94%

Citrulline reduces glyceroneogenesis and induces fatty acid release in visceral adipose tissue from overweight rats

Joffin

Jaubert

Durant

et al. 2014

Molecular Nutrition Food Res

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“…The TNF-α level and IL-8 production induced the expression of many other inflammatory mediators that damage vascular integrity [31]. Therefore, the suppression of inflammatory cytokines is a potential therapeutic strategy for vascular disease.…”

Section: Discussionmentioning

confidence: 99%

Vitamin D Ameliorates Angiotensin II-Induced Human Endothelial Progenitor Cell Injury via the PPAR-γ/HO-1 Pathway

et al. 2019

J Vasc Res

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Vitamin D has an important protective effect on chronic inflammatory disease. Angiotensin II (AngII) triggers vascular damage and plays a key role in vascular diseases via several mechanisms, including inflammation. Conversely, vitamin D has been shown to have an important protective effect on chronic inflammation. There is evidence showing that vitamin D can reverse the effects of AngII, but the molecular mechanisms by which this occurs are not known. Our results demonstrate that vitamin D improved the viability, migration ability, and tube formation of AngII-pretreated endothelial progenitor cells (EPCs) and inhibited the apoptosis of EPCs induced by AngII. Vitamin D also reversed reactive oxygen species production, vascular inflammatory cytokine generation, and nuclear factor kappa-B activation in EPCs induced by AngII. Furthermore, EPC pretreatment with GW9662 (the antagonist for PPAR-γ) or siHO-1 decreased the protective effect of vitamin D on AngII-induced EPC injury. Overall, our data indicate that vitamin D ameliorated AngII-induced abnormal EPC injury by decreasing oxidative stress and inflammatory cytokine levels. These findings also suggest that vitamin D protected EPCs from AngII-induced vascular injury via the activation of the PPAR-γ/HO-1 signaling pathway.

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“…The needle was connected to a 500 ml container of Sodium Lactate Ringer's Injection or irrigation solution containing 5 mM Ala-Gln for the anterior chamber perfusion. The concentration of Ala-Gln was selected according to previous studies (Haynes et al, 2009;Raspe et al, 2013). The mice were randomized during the perfusion with either Sodium Lactate Ringer's Injection or Sodium Lactate Ringer's Injection containing 5 mmol/L Ala-Gln for 60 minutes.…”

Section: Intracameral Irrigation Modelmentioning

confidence: 99%

Protective Effects Oncorneal Endothelium During Intracameral Irrigation Using N-(2)-l-alanyl-l-Glutamine

Jin

Wang

et al. 2020

Front. Pharmacol.

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Corneal endothelial disease is a global sight-threatening disease, and corneal transplantation using donor corneas remains the sole therapeutic option. A previous work demonstrated that N (2)-alanyl-glutamine (Ala-Gln) protected against apoptosis and cellular stress, and maintained intestinal tissue integrity. In this pursuit, the present study aimed to examine the effect of Ala-Gln in the protection of the corneal endothelium and expand its range of potential clinical applications. Mice in the control group were intracamerally irrigated with Ringers lactate injection, whereas those in the experimental group were irrigated with Ringers lactate injection containing Ala-Gln. The mean intraocular pressure increased to 44 ± 3.5 mm Hg during intracameral irrigation (normal range 10.2 ± 0.4 mmHg). In vivo confocal microscopy results showed that the addition of Ala-Gln protected the morphology, structure, and density of the corneal endothelial cells. Optical Coherence Tomography (OCT) measurements showed that corneal thickness was not significantly different between the two groups, because of the immediate corneal edema after irrigation, but the addition of Ala-Gln obviously promoted the recovery of the corneal edema. Scanning electron microscopy indicated that the corneal endothelial cells were severely ruptured and exfoliated in the Ringer's group accompanied with cellular edema, when compared with the Ala-Gln group. The intracameral irrigation using Ala-Gln protected the structure and expression of cytoskeleton and Na-K-ATPase, which exhibited a regular distribution and significantly increased expression in comparison to Ringer's group. Furthermore, Ala-Gln maintained the mitochondrial morphology and increased the activity of mitochondria. Moreover, transmission electron microscopy showed that intracameral irrigation of Ala-Gln reversed the ultrastructural changes induced by the acute ocular hypertension in mice. Our study demonstrates that the intracameral irrigation of Ala-Gln effectively maintained the corneal endothelial pump function and barrier function by protecting the mitochondrial function and preventing the rearrangement of cytoskeleton in acute ocular hypertension in mice.

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Glutamine and alanine-induced differential expression of intracellular IL-6, IL-8, and TNF-α in LPS-stimulated monocytes in human whole-blood

Cited by 26 publications

References 25 publications

Citrulline reduces glyceroneogenesis and induces fatty acid release in visceral adipose tissue from overweight rats

Citrulline reduces glyceroneogenesis and induces fatty acid release in visceral adipose tissue from overweight rats

Vitamin D Ameliorates Angiotensin II-Induced Human Endothelial Progenitor Cell Injury via the PPAR-γ/HO-1 Pathway

Protective Effects Oncorneal Endothelium During Intracameral Irrigation Using N-(2)-l-alanyl-l-Glutamine

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