Glutaraldehyde-induced states of stress of the collagen triple helix

Jonak, R.; Nemetschek-Gansler, H.; Nemetschek, Th.; Riedl, Harald Udo von; Bordas, J.; Koch, Marta

doi:10.1016/0022-2836(79)90438-8

Cited by 14 publications

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“…After cross-linking with glutaraldehyde, the amide I peaks positions shifted to 1642.6 cm − 1 and the reduced intensity indicates the conformational change in triple helix. 30 – 32 The amide II peak position also shifted towards higher wavenumber from 1554.26 to 1571.78 cm − 1 and increased intensity, indicating that amino (-NH 2 ) groups of the lysine residue of collagen react with aldehyde (-CHO) groups of glutaraldehyde to form the aldimine linkage (CH=N). 33 The peaks at 1452.67 cm − 1 disappeared and 1410.18 cm − 1 transformed into an intense peak suggesting an increase in symmetric CH 3 bending vibration.…”

Section: Resultsmentioning

confidence: 99%

Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration

et al. 2021

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Current gold standard to treat soft tissue injuries caused by trauma and pathological condition are autografts and off the shelf fillers, but they have inherent weaknesses like donor site morbidity, immuno-compatibility and graft failure. To overcome these limitations, tissue-engineered polymers are seeded with stem cells to improve the potential to restore tissue function. However, their interaction with native tissue is poorly understood so far. To study these interactions and improve outcomes, we have fabricated scaffolds from natural polymers (collagen, fibrin and elastin) by custom-designed processes and their material properties such as surface morphology, swelling, wettability and chemical cross-linking ability were characterised. By using 3D scaffolds, we comprehensive assessed survival, proliferation and phenotype of adipose-derived stem cells in vitro. In vivo, scaffolds were seeded with adipose-derived stem cells and implanted in a rodent model, with X-ray microtomography, histology and immunohistochemistry as read-outs. Collagen-based materials showed higher cell adhesion and proliferation in vitro as well as higher adipogenic properties in vivo. In contrast, fibrin demonstrated poor cellular and adipogenesis properties but higher angiogenesis. Elastin formed the most porous scaffold, with cells displaying a non-aggregated morphology in vitro while in vivo elastin was the most degraded scaffold. These findings of how polymers present in the natural polymers mimicking ECM and seeded with stem cells affect adipogenesis in vitro and in vivo can open avenues to design 3D grafts for soft tissue repair.

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Section: Resultsmentioning

confidence: 99%

Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration

et al. 2021

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Solvent environment modulates effects of glutaraldehyde crosslinking on tissue-derived biomaterials

Gratzer

Lee

1996

J. Biomed. Mater. Res.

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Bioprosthetic materials utilized in the construction of heart valves and vascular grafts possess limited performance and viability in vivo. This is due (in part) to the failure of these materials to mimic the mechanical properties of the host tissue they replace. If bioprosthetic materials could be engineered to meet the mechanical performance required in vivo, the functional lifetime of implants would be increased. In this study, glutaraldehyde/solvent solutions of decreasing dielectric constant (polarity) were utilized to modify the properties of crosslinked collagen in whole bovine pericardial tissue. Solvents included phosphate buffer, methanol, 95% (w/w) ethanol, n-propanol, and n-butanol. Exogenous crosslinking was verified in collagen by thermal denaturation tests and amino acid analyses. Tensile mechanical behavior of collagenous pericardial samples was found to depend upon the dielectric constant (polarity) of the glutaraldehyde/solvent solutions employed; however, treatment in the solvents alone had little, if any, effect. As the dielectric constant of the solvents decreased, three mechanical properties were systematically altered: plastic strain fell from a mean of 8.9 +/- 1.5% (buffer) to 1.6 +/- 0.4% (n-butanol); strain at fracture increased from 32.2 +/- 2.6% (buffer) to 55.6 +/- 4.6% (n-butanol); and percent stress remaining after 1000-s stress relaxation from an 80-g initial load fell from 86.3 +/- 1.1% (buffer) to 76.9 +/- 1.0% (n-butanol). Crosslinking using a glutaraldehyde/n-butanol solution produced materials with tensile mechanical behavior that was very close to that of fresh tissue; however, the flexural properties of the treated tissue were different from those of fresh tissue. This decoupling of the flexural and tensile mechanical behaviors of crosslinked bioprosthetic materials is unique to this form of treatment. The observed phenomena may be the results of conformational changes in collagen facilitated by polar/nonpolar interactions with the solvent that are "locked in" by the action of glutaraldehyde. This technique may aid in the "customized" design of mechanical properties in tissue-derived biomaterials.

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Strukturdynamik isocyanatvernetzter Kollagenfasern; Anwendung der Synchrotronstrahlung in der Makromolekularen Chemie

et al. 1987

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Intermolekulare und interfibrilläre Vernetzungen experimentell quantitativ zu unterscheiden ist jetzt erstmals bei Kollagenfasern gelungen. Entscheidend war, daß die Vernetzung nicht mit Dialdehyden, sondern mit einem Diisocyanat durchgeführt wurde, und die Verwendung von Synchrotronstrahlung. Das Ausmaß der intermolekularen Vernetzung hängt von der Reaktionszeit ab. Die Befunde dürften beispielhaft nicht nur für Kollagenfasern, sondern auch für andere, einer strukturdynamischen Röntgenbeugungsanalyse zugängliche faserige Bio‐ und Hochpolymere sein.

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Glutaraldehyde-induced states of stress of the collagen triple helix

Cited by 14 publications

References 1 publication

Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration

Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration

Solvent environment modulates effects of glutaraldehyde crosslinking on tissue-derived biomaterials

Strukturdynamik isocyanatvernetzter Kollagenfasern; Anwendung der Synchrotronstrahlung in der Makromolekularen Chemie

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