Glutaredoxin like protein (RtGRL1) regulates H2O2 and Na+ accumulation by maintaining the glutathione pool during abiotic stress

Ma, Binjie; Suo, Yafei; Zhang, Jie; Xing, Ningning; Gao, Zheng; Lin, Xiaofei; Zheng, Linlin; Wang, Yingchun

doi:10.1016/j.plaphy.2020.11.040

Cited by 16 publications

(5 citation statements)

References 57 publications

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“…GRX is involved in glutathione-dependent detoxification of H 2 O 2 , and MDAR participates in the ascorbate−glutathione cycle, which removes toxic H 2 O 2 . 19,53 These results reveal that the MebZIP5/ MebZIP34−MeGRX6/MeMDAR1 module may be involved in ABA-induced antioxidative capacity during PPD.…”

Section: ■ Discussionmentioning

confidence: 75%

“…Moreover, exogenous ABA significantly induced the expression of MebZIP5/MebZIP34 and MeGRX6/MeMDAR1 , the increase in GRX content and MDAR activity, and the decrease in H 2 O 2 content during the postharvest period of cassava (Figures D, E, and ). GRX is involved in glutathione-dependent detoxification of H 2 O 2 , and MDAR participates in the ascorbate–glutathione cycle, which removes toxic H 2 O 2 . , These results reveal that the MebZIP5/MebZIP34–MeGRX6/MeMDAR1 module may be involved in ABA-induced antioxidative capacity during PPD.…”

Section: Discussionmentioning

confidence: 99%

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Abscisic Acid Signaling in the Regulation of Postharvest Physiological Deterioration of Sliced Cassava Tuberous Roots

Yan

Zhao

et al. 2022

J. Agric. Food Chem.

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Phytohormone abscisic acid (ABA) influences the shelf life of fruit, vegetables, and tubers after harvest. However, little is known about the core signaling module involved in ABA's control of the postharvest physiological process. Exogenous ABA alleviated postharvest physiological deterioration (PPD) symptoms of sliced cassava tuberous roots, increased endogenous ABA levels, and reduced endogenous H 2 O 2 content. The specific ABA signaling module during the PPD process was identified as MePYL6−MePP2C16−MeSnRK2.1−MebZIP5/34. MebZIP5/MebZIP34 directly binds to and activates the promoters of MeGRX6/MeMDAR1 through ABRE elements. Exogenous ABA significantly induced the expression of genes involved in this module, glutaredoxin content, and monodehydroascorbate reductase activity. We presented a hypothesis suggesting that MePYL6− MePP2C16−MeSnRK2.1−MebZIP5/34−MeGRX6/MeMDAR1 is involved in ABA-induced antioxidative capacity, thus alleviating PPD symptoms in cassava tuberous roots. The identification of the specific signaling module involved in ABA's control of PPD provides a basis and potential targets for extending the shelf life of cassava tuberous roots.

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Section: ■ Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Abscisic Acid Signaling in the Regulation of Postharvest Physiological Deterioration of Sliced Cassava Tuberous Roots

Yan

Zhao

et al. 2022

J. Agric. Food Chem.

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“…CAT mainly exists in peroxidase bodies in plant cells, which can not only catalyze the H 2 O 2 reaction, but also oxidize other small molecules of toxic substances. H 2 O 2 , as an important form of ROS, can be scavenged by CAT 27,28 .In addition, some studies have shown that GSH is involved in controlling intracellular level of H 2 O 2 29,30 . In this study, the level of H 2 O 2 in F-GSH-treated cells decreased after exogenous addition of GSH and increased after exogenous addition of BSO.…”

Section: Discussionmentioning

confidence: 99%

Reactive oxygen metabolism in the proliferation of Korean pine embryogenic callus cells promoted by exogenous GSH

Gao

Wang

Shi

et al. 2023

Sci Rep

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Exogenous glutathione (GSH) promotes the proliferation of embryogenic callus (EC) cells in Korean pine in the course of somatic embryogenesis, and reactive oxygen species (ROS) may play an important role in regulating the proliferation of EC cells by exogenous GSH. However, the concrete metabolic response of ROS is unclear. In this study, two cell lines of Korean pine with high proliferative potential 001#-001 (F, Fast proliferative potential cell line is abbreviated as F) and low proliferative potential 001#-010 (S, Slow proliferative potential cell line is abbreviated as S) were used as test materials. The responses of ROS-related enzymes and substances to exogenous GSH and L-Buthionine-sulfoximine (BSO) were investigated in EC cells. The results showed that the exogenous addition of GSH increased the number of early somatic embryogenesis (SEs) in EC cells of both F and S cell lines, decreased the amount of cell death in both cell lines. Exogenous addition of GSH promoted cell division in both cell lines, increased intracellular superoxide dismutase (SOD) and catalase (CAT) activities, inhibited intracellular hydrogen peroxide (H2O2), malondialdehyde (MDA) and nitric oxide (NO) production, and increased NO/ROS ratio. In conclusion, the exogenous GSH promoting the proliferation of Korean pine EC cells, the activity of intracellular antioxidant enzymes was enhanced, the ROS level was reduced, and the resistance of cells to stress was enhanced.

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“…At the same time, NO can also enhance the production of antioxidant enzymes through the MAPK pathway [ 34 ], and can also enhance the accumulation of antioxidant enzymes through the S-nitroso (SNO) process [ 35 ]. As a strong oxidant, GSH can directly remove ROS in cells ( Figure 8 ), and detoxify ROS by assisting GSH–ASA circulation [ 36 ]. In this study, the spraying of exogenous NO and GSH can significantly enhance the activities of SOD, POD, CAT, APX, DHAR, and MDHAR in cowpea seedlings, and the effect of NO+GSH treatment was the best ( Figure 5 and Figure 6 ), indicating that NO and GSH could synergically alleviate the stress of low temperature on cowpea seedlings, which is consistent with the research results of Yao et al [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

NO and GSH Alleviate the Inhibition of Low-Temperature Stress on Cowpea Seedlings

Song

Zhang

et al. 2023

Plants

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Low-temperature stress in early spring seriously affects the growth and development of cowpea seedlings. To study the alleviative effect of the exogenous substances nitric oxide (NO) and glutathione (GSH) on cowpea (Vigna unguiculata (Linn.) Walp.) seedlings under 8 °C low-temperature stress, 200 μmol·L−1 NO and 5 mmol·L−1 GSH were sprayed on cowpea seedlings whose second true leaf was about to unfold to enhance the tolerance of cowpea seedlings to low temperature. Spraying NO and GSH can eliminate excess superoxide radicals (O2−) and hydrogen peroxide (H2O2) to varying degrees, reduce the content of malondialdehyde and relative conductivity, delay the degradation of photosynthetic pigments, increase the content of osmotic regulating substances such as soluble sugar, soluble protein, and proline, and improve the activity of antioxidant enzymes such as superoxide dismutase, peroxidase, catalase, ascorbate peroxidase, dehydroascorbate reductase, and monodehydroascorbate reductase. This study revealed that the mixed use of NO and GSH played an important role in alleviating low temperature stress, and the effect of spraying NO alone was better than that of spraying GSH.

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Glutaredoxin like protein (RtGRL1) regulates H2O2 and Na+ accumulation by maintaining the glutathione pool during abiotic stress

Cited by 16 publications

References 57 publications

Abscisic Acid Signaling in the Regulation of Postharvest Physiological Deterioration of Sliced Cassava Tuberous Roots

Abscisic Acid Signaling in the Regulation of Postharvest Physiological Deterioration of Sliced Cassava Tuberous Roots

Reactive oxygen metabolism in the proliferation of Korean pine embryogenic callus cells promoted by exogenous GSH

NO and GSH Alleviate the Inhibition of Low-Temperature Stress on Cowpea Seedlings

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