Glutathione-s-transferase modified electrodes for detecting anticancer drugs

Materón, Elsa M.; Huang, Po‐Jung Jimmy; Wong, Ademar; Ferreira, A. A.; Sotomayor, Marı́a Del Pilar Taboada; Liu, Juewen

doi:10.1016/j.bios.2014.02.070

Cited by 42 publications

(23 citation statements)

References 29 publications

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“…CDNB is widely used in quantification of the glutathione-s-transferase enzyme [13][14][15][16] and its substrates [17]. However, despite its environmental and health relevance, only very few reports have been published concerning CDNB quantification [18].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of a new magnetic-MIP for the selective detection of 1-chloro-2,4-dinitrobenzene, a highly allergenic compound

Uzuriaga-Sánchez

Wong

Khan

et al. 2017

Materials Science and Engineering: C

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Molecularly imprinted polymers (MIPs) in combination with magnetic nanoparticles, in a core@shell format, were studied for selective detection of 1-chloro-2,4-dinitrobenzene (CDNB), a powerful allergenic substance. Magnetic nanoparticles were prepared by the co-precipitation method and mixed with oleic acid (OA). This material was then encapsulated in three types of hydrophobic polymeric matrix, poly-(MA-co-EDGMA), poly-(AA-co-EDGMA), and poly-(1-VN-co-EDGMA), by the mini-emulsion method. These matrices were used due to their ability to interact specifically with the functional groups of the analyte. Finally, the MIP-CDNB was obtained on the magnetic-hydrophobic surfaces using precipitation polymerization in the presence of the analyte. XRD diffraction patterns suggested the presence of magnetite in the composite and SEM analysis revealed a nanoparticle size between 10 and 18nm. Under the optimized adsorption conditions, the magnetic-MIP material showed a higher adsorption capacity (5.1mgg) than its non-magnetic counterpart (4.2mgg). In tests of the selectivity of the magnetic-MIP towards CDNB, α-values of 2.5 and 10.4, respectively, were obtained for dichlorophenol and o-nitrophenol, two structurally similar compounds, and no adsorption was observed for any other non-analogous analyte. The magnetic-MIP and magnetic-NIP were applied using water enriched with 0.5mgL of CDNB, achieving recovery values of 83.8(±0.8)% and 66(±1)%, respectively, revealing the suitability of the material for detection of CDNB.

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Section: Introductionmentioning

confidence: 99%

Synthesis of a new magnetic-MIP for the selective detection of 1-chloro-2,4-dinitrobenzene, a highly allergenic compound

Uzuriaga-Sánchez

Wong

Khan

et al. 2017

Materials Science and Engineering: C

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“…More than 80% of patients initially respond to DDP, but the acquired drug resistance in most patients with advanced disease finally leads to relapse and death (3). Although multiple factors and mechanisms, including abnormity of drug efflux, drug target, DNA repair, and apoptosis pathways (4–6), have been reported to contribute to DDP resistance, the underlying mechanisms for DDP resistance are still poorly understood.…”

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confidence: 99%

RCC2, a regulator of the RalA signaling pathway, is identified as a novel therapeutic target in cisplatin‐resistant ovarian cancer

et al. 2019

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Currently, cisplatin (DDP) is the first‐line chemotherapeutic agent used for treatment of ovarian cancer, but gradually acquired drug resistance minimizes its therapeutic outcomes. We aimed to identify crucial genes associated with DDP resistance in ovarian cancer and uncover potential mechanisms. Two sets of gene expression data were downloaded from Gene Expression Omnibus, and bioinformatics analysis was conducted. In our study, the differentially expressed genes between DDP‐sensitive and DDP‐resistant ovarian cancer were screened in GSE15709 and GSE51373 database, and chromosome condensation 2 regulator (RCC2) and nucleoporin 160 were identified as 2 genes that significantly up‐regulated in DDP‐resistant ovarian cancer cell lines compared with DDP‐sensitive cell lines. Moreover, RCC2, Ral small GTPase (RalA), and Ral binding protein‐1 (RalBP1) expression was found to be significantly higher in DDP‐resistant ovarian cancer tissues than in DDP‐sensitive tissues. RCC2 plays a positive role in cell proliferation, apoptosis, and migration in DDP‐resistant ovarian cancer cell lines in vitro and in vivo. Furthermore, RCC2 could interact with RalA, thus promoting its downstream effector RalBP1. RalA knockdown could reverse the effects of RCC2 overexpression on DDP‐resistant ovarian cancer cell proliferation, apoptosis, and migration. Similarly, RalA overexpression could alleviate the effects of RCC2 knockdown in DDP‐resistant ovarian cancer cells. Taken together, RCC2 may function as an oncogene, regulating the RalA signaling pathway, and intervention of RCC2 expression might be a promising therapeutic strategy for DDP‐resistant ovarian cancer. —Gong, S., Chen, Y., Meng, F., Zhang, Y., Wu, H., Li, C., Zhang, G. RCC2, a regulator of the RalA signaling pathway, is identified as a novel therapeutic target in cisplatin‐resistant ovarian cancer. FASEB J. 33, 5350–5365 (2019). http://www.fasebj.org

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“…Molecules that inhibit GST are therefore considered candidates for new anticancer drug [23]. The GST-catalyzed GSH-CDNB reaction can be followed with AuNPs as colorimetric probes, and one can easily use the A 670 /A 520 ratio to evaluate whether the molecule can inhibit GST activity and compare the inhibition efficiency among different inhibitors.…”

Section: Resultsmentioning

confidence: 99%

Simple method for visual detection of glutathione S-transferase activity and inhibition using cysteamine-capped gold nanoparticles as colorimetric probes

Ren

Zhou

et al. 2015

Gold Bull

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An operationally simple colorimetric method for measuring glutathione S-transferase (GST) activity was developed using cysteamine-capped gold nanoparticles (AuNPs) in this work. This method was based that GST can catalyze the reaction of 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione (GSH) to produce a conjugate (CDNB-SG). GSH could induce the aggregation of AuNPs, whereas the conjugate could not induce the aggregation of AuNPs. Thus, GST activity can be facilely assayed with the naked eye or a simple colorimetric reader. The convenient and simple colorimetric response of the assay makes them an attractive approach for drug-screening application.

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Glutathione-s-transferase modified electrodes for detecting anticancer drugs

Cited by 42 publications

References 29 publications

Synthesis of a new magnetic-MIP for the selective detection of 1-chloro-2,4-dinitrobenzene, a highly allergenic compound

Synthesis of a new magnetic-MIP for the selective detection of 1-chloro-2,4-dinitrobenzene, a highly allergenic compound

RCC2, a regulator of the RalA signaling pathway, is identified as a novel therapeutic target in cisplatin‐resistant ovarian cancer

Simple method for visual detection of glutathione S-transferase activity and inhibition using cysteamine-capped gold nanoparticles as colorimetric probes

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