Glycan and lectin microarrays for glycomics and medicinal applications

Katrlı́k, Jaroslav; Švitel, Juraj; Gemeiner, Peter; Kožár, Tibor; Tkac, Jan

doi:10.1002/med.20195

Cited by 91 publications

(73 citation statements)

References 142 publications

(323 reference statements)

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“…Lectin and glycan microarrays are two of the key tools used in laboratories to screen molecules and cells for glycans or lectins respectively [20]. Arrays differ in the range of glycans/lectins and the manner in which they are displayed on the chip surfaces as this can be an influencing factor in the recognition of the glycan [21].…”

Section: Characterization Of Carbohydrate-lectin Interactionsmentioning

confidence: 99%

Exploitation of Glycobiology in Anti-Adhesion Approaches against Biothreat Agents

Utratna¹,

Deegan²,

Joshi³

2016

J Bioterror Biodef

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Pathogen adherence to a host cell is one of the first essential steps for establishing invasion, colonization and release of virulence factors such as toxins. Understanding the mechanisms used by pathogens and toxins to adhere and invade human cells could lead to the development of new strategies for preventing and controlling the spread of infectious diseases. This review focuses on carbohydrate-lectin interactions utilized by selected biothreat agents to bind and invade host cells. The principle of using anti-adhesion molecules, based on glycobiology research, has already been shown to be effective in the treatment of influenza. Therefore, translating the same principle to other biothreat agents that mediate invasion of a host cell through carbohydrate-lectin mechanisms is a very promising strategy. We investigate recent literature to highlight the latest developments in the field of glycobiology focused on inhibiting the initial steps of pathogen invasion, with examples for bacteria, toxin and virus interactions. The successful glycomimetics and glycoconjugates represent strategies for interruption of adhesion by single molecules and in multivalent systems against uropathogenic E. coli, several toxins (Shiga-like, cholera, botulinum) and wellknown or emerging viruses (influenza, HIV, Ebola, and Zika). This review provides promising directions and prophylactic as well as therapeutic potential of anti-adhesive strategies against selected biothreat targets.

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Section: Characterization Of Carbohydrate-lectin Interactionsmentioning

confidence: 99%

Exploitation of Glycobiology in Anti-Adhesion Approaches against Biothreat Agents

Utratna¹,

Deegan²,

Joshi³

2016

J Bioterror Biodef

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“…It is well-known that presence of labels can negatively affect binding [24,25] and thus label-free methods of analysis are preferential option for diagnostic purposes. One of the most sensitive biosensing detection platform is electrochemical impedance spectroscopy (EIS), offering robust, extremely sensitive method for monitoring of biorecognition events in a label-free mode of operation [19,[26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Glycoprofiling as a novel tool in serological assays of systemic sclerosis: A comparative study with three bioanalytical methods

Kluková

Bertók

Petrikova

et al. 2015

Analytica Chimica Acta

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Systemic sclerosis (SSc) is an autoimmune disease seriously affecting patient´s quality of life. The heterogeneity of the disease also means that identification and subsequent validation of biomarkers of the disease is quite challenging. A fully validated single biomarker for diagnosis, prognosis, disease activity and assessment of response to therapy is not yet available. The main aim of this study was to apply an alternative assay protocol to the immunoassay-based analysis of this disease by employment of sialic acid recognizing lectin Sambucus nigra agglutinin (SNA) to glycoprofile serum samples. To our best knowledge this is the first study describing direct lectin-based glycoprofiling of serum SSc samples. Three different analytical methods for glycoprofiling of serum samples relying on application of lectins are compared here from a bioanalytical point of view including traditional ELISA-like lectin-based method (ELLA), novel fluorescent lectin microarrays and ultrasensitive impedimetric lectin biosensors. Results obtained by all three bioanalytical methods consistently showed differences in the level of sialic acid present on glycoproteins, when serum from healthy people was compared to the one from patients having SSc. Thus, analysis of sialic acid content in human serum could be of a diagnostic value for future detection of SSc, but further work is needed to enhance selectivity of assays for example by glycoprofiling of a fraction of human serum enriched in antibodies for individual diagnostics.

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“…(9,10). Glycans present on the surface of cells are naturally involved in pathological processes including viral and bacterial infections, in neurological disorder and in tumour growth and metastasis (3,(11)(12)(13)(14)(15)(16). Thus, better understanding of glycan mediated pathogenesis is essential in order to establish a "policy" to develop efficient routes for disease treatment with several recent studies as good examples e.g.…”

Section: Introductionmentioning

confidence: 99%

“…There is an alternative way for glycoprofiling by application of lectins, natural glycan recognising proteins (28, 38,39) in combination with various transducing protocols (12,40,41). The most powerful glycoprofiling tool relies on lectins arrayed on solid surfaces for direct analysis of glycoproteins, glycolipids, membranes and even glycans on the surface of intact cells (11,42,12,43).…”

Section: Introductionmentioning

confidence: 99%