Glycan microarrays from construction to applications

Kim, Yujun; Hyun, Ji Young; Shin, Injae

doi:10.1039/d2cs00452f

Cited by 20 publications

(15 citation statements)

References 289 publications

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“…The interaction of glycans with glycan-binding proteins (GBP) helps in understanding the molecular mechanisms of various immunological events [ 172 , 173 , 174 ]. The first reported glycan microarray in the year 2002 [ 175 , 176 ] studied GBP events using a small amount of a glycan sample [ 28 , 29 , 177 ]. Later years witnessed the use of glycan microarrays in assessing glycan-processing enzyme characterization, the discovery of functional glycans, drug discovery, studying weak carbohydrate interactions, and pathogen diagnosis [ 76 , 178 , 179 , 180 ].…”

Section: Application Of Microarraysmentioning

confidence: 99%

“…Over the years, from time to time, various review articles or book chapters have been published on both the technological advancements and applications of microarrays in various fields. In the case of biomolecule-based microarrays, the focus of those research articles was majorly restricted to an individual array, i.e., a DNA microarray [ 20 , 21 , 22 , 23 ], protein microarray [ 24 , 25 , 26 ], glycan microarray [ 27 , 28 , 29 ], peptide microarray [ 30 , 31 , 32 ], or antibody microarray [ 13 , 33 , 34 ]. The truncation of the microarray technology reviews based on individual biomolecules has its own advantages (serves the purpose of those specialized in one particular subdomain or a more focused area).…”

Section: Introductionmentioning

confidence: 99%

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Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

Tetala

2023

Biomolecules

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Microarrays are one of the trailblazing technologies of the last two decades and have displayed their importance in all the associated fields of biology. They are widely explored to screen, identify, and gain insights on the characteristics traits of biomolecules (individually or in complex solutions). A wide variety of biomolecule-based microarrays (DNA microarrays, protein microarrays, glycan microarrays, antibody microarrays, peptide microarrays, and aptamer microarrays) are either commercially available or fabricated in-house by researchers to explore diverse substrates, surface coating, immobilization techniques, and detection strategies. The aim of this review is to explore the development of biomolecule-based microarray applications since 2018 onwards. Here, we have covered a different array of printing strategies, substrate surface modification, biomolecule immobilization strategies, detection techniques, and biomolecule-based microarray applications. The period of 2018–2022 focused on using biomolecule-based microarrays for the identification of biomarkers, detection of viruses, differentiation of multiple pathogens, etc. A few potential future applications of microarrays could be for personalized medicine, vaccine candidate screening, toxin screening, pathogen identification, and posttranslational modifications.

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Section: Application Of Microarraysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

Tetala

2023

Biomolecules

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“…Furthermore, the IR laser capabilites to form a bubble to displace trapped cell/particle Deformability analysis of RBCs using micro-capillaries has shown experimental observations based on the RBC dynamics to detect cellular subpopulations [171]. Other prospective studies include, RBC shape assessment [313], malaria screening [314][315][316][317][318], analysis of microcapillary occlusionsickle cell disease [319][320][321][322], sickle cell disease [323,324], detection and labeling of lymphoma cells [325], blood grouping and phenotyping [326,327], and the study of dispersive RBCs flowing through microchannels [328].…”

Section: The Concept Of Microarraysmentioning

confidence: 99%

Advances in Microfluidics for Single Red Blood Cell Analysis

et al. 2023

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The utilizations of microfluidic chips for single RBC (red blood cell) studies have attracted great interests in recent years to filter, trap, analyze, and release single erythrocytes for various applications. Researchers in this field have highlighted the vast potential in developing micro devices for industrial and academia usages, including lab-on-a-chip and organ-on-a-chip systems. This article critically reviews the current state-of-the-art and recent advances of microfluidics for single RBC analyses, including integrated sensors and microfluidic platforms for microscopic/tomographic/spectroscopic single RBC analyses, trapping arrays (including bifurcating channels), dielectrophoretic and agglutination/aggregation studies, as well as clinical implications covering cancer, sepsis, prenatal, and Sickle Cell diseases. Microfluidics based RBC microarrays, sorting/counting and trapping techniques (including acoustic, dielectrophoretic, hydrodynamic, magnetic, and optical techniques) are also reviewed. Lastly, organs on chips, multi-organ chips, and drug discovery involving single RBC are described. The limitations and drawbacks of each technology are addressed and future prospects are discussed.

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“…Consequently, techniques that allow fast and reliable characterization of carbohydrate‐lectin interactions are of a great importance in glycomics. In this context, glycan microarray technology has revolutionized the field and has become an essential technique for the high throughput study of binding affinities GBPs and glycans [2–5] . However, hampered by the access to large libraries of pure glycan structures it remains difficult to take full advantage of the available glycan array platforms.…”

Section: Introductionmentioning

confidence: 99%

“…In this context, glycan microarray technology has revolutionized the field and has become an essential technique for the high throughput study of binding affinities GBPs and glycans. [2][3][4][5] However, hampered by the access to large libraries of pure glycan structures it remains difficult to take full advantage of the available glycan array platforms. Robust access to well characterized complex glycans in sufficient amounts is granted by chemo-enzymatic synthesis but this approach is time consuming and laborious and requires highly experienced personnel.…”

Section: Introductionmentioning

confidence: 99%

A Versatile Urea Type Linker for Functionalizing Natural Glycans and Its Validation in Glycan Arrays

Serna

Artschwager

Pérez-Martínez

et al. 2023

Chemistry A European J

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The isolation from organisms and readily available glycoproteins has become an increasingly convenient source of N‐glycans for multiple applications including glycan microarrays, as reference standards in glycan analysis or as reagents that improve bioavailability of protein and peptide therapeutics through conjugation. A problematic step in the isolation process on a preparative scale can be the attachment of a linker for the improved purification, separation, immobilization and quantification of the glycan structures. Addressing this issue, we firstly aimed for the development of an UV active linker for a fast and reliable attachment to anomeric glycosylamines via urea bond formation. Secondly, we validated the new linker on glycan arrays in a comparative study with a collection of N‐glycans which were screened against various lectins. In total, we coupled four structurally varied N‐glycans to four different linkers, immobilized all constructs on a microarray and compared their binding affinities to four plant and fungal lectins of widely described specificity. Our study shows that the urea type linker showed an overall superior performance for lectin binding and once more, highlights the often neglected influence of the choice of linker on lectin recognition.

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Glycan microarrays from construction to applications

Abstract: This article summarizes the preparation of glycan probes, the construction of glycan microarrays and their applications for biological and biomedical research.

Cited by 20 publications

References 289 publications

Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

Advances in Microfluidics for Single Red Blood Cell Analysis

A Versatile Urea Type Linker for Functionalizing Natural Glycans and Its Validation in Glycan Arrays

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