Glycan profiling analysis using evanescent-field fluorescence-assisted lectin array: Importance of sugar recognition for cellular uptake of exosomes from mesenchymal stem cells

Shimoda, Asako; Tahara, Yoshiro; Sawada, Shin–ichi; Sasaki, Yoshihiro; Akiyoshi, Kazunari

doi:10.1016/j.bbrc.2017.07.126

Cited by 116 publications

(110 citation statements)

References 39 publications

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“…Interestingly, it has been found that the latter is the main responsible for the therapeutic properties of the conditioned media from MSC cultures (Kusuma et al, 2017). This way, those EV can regulate different physiological processes, like cellular proliferation, differentiation, and migration (Shimoda et al, 2017;Zou et al, 2018).…”

Section: Properties and Therapeutic Potential Of Mscmentioning

confidence: 99%

Extracellular Vesicles Derived From Mesenchymal Stem Cells (MSC) in Regenerative Medicine: Applications in Skin Wound Healing

Casado-Díaz

Quesada‐Gómez

Dorado

2020

Front. Bioeng. Biotechnol.

232

183

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The cells secrete extracellular vesicles (EV) that may have an endosomal origin, or from evaginations of the plasma membrane. The former are usually called exosomes, with sizes ranging from 50 to 100 nm. These EV contain a lipid bilayer associated to membrane proteins. Molecules such as nucleic acids (DNA, mRNA, miRNA, lncRNA, etc.) and proteins may be stored inside. The EV composition depends on the producer cell type and its physiological conditions. Through them, the cells modify their microenvironment and the behavior of neighboring cells. That is accomplished by transferring factors that modulate different metabolic and signaling pathways. Due to their properties, EV can be applied as a diagnostic and therapeutic tool in medicine. The mesenchymal stromal cells (MSC) have immunomodulatory properties and a high regenerative capacity. These features are linked to their paracrine activity and EV secretion. Therefore, research on exosomes produced by MSC has been intensified for use in cell-free regenerative medicine. In this area, the use of EV for the treatment of chronic skin ulcers (CSU) has been proposed. Such sores occur when normal healing does not resolve properly. That is usually due to excessive prolongation of the inflammatory phase. These ulcers are associated with aging and diseases, such as diabetes, so their prevalence is increasing with the one of such latter disease, mainly in developed countries. This has very important socio-economic repercussions. In this review, we show that the application of MSC-derived EV for the treatment of CSU has positive effects, including accelerating healing and decreasing scar formation. This is because the EV have immunosuppressive and immunomodulatory properties. Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. They include endothelial cells, fibroblasts, and keratinocytes. Most of the studies carried out so far are preclinical. Therefore, there is a need to advance more in the knowledge about the conditions of production, isolation, and action mechanisms of EV. Interestingly, their potential application in the treatment of CSU opens the door for the design of new highly effective therapeutic strategies.

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Section: Properties and Therapeutic Potential Of Mscmentioning

confidence: 99%

Extracellular Vesicles Derived From Mesenchymal Stem Cells (MSC) in Regenerative Medicine: Applications in Skin Wound Healing

Casado-Díaz

Quesada‐Gómez

Dorado

2020

Front. Bioeng. Biotechnol.

232

183

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“…Galectin 5 on reticulocytes is involved in uptake by macrophages (58). Siglecs, another lectin subcategory of proteins, are seen responding to exosomes with the interaction of CD169 on macrophages and B-cell exosomal a-2,3-linked sialic acid (89) and Siglec-3 on HeLa cells or APCs and sialic acid on stem cell-derived exosomes (90). Saunderson and colleagues described the dependence of B-cell and dendritic cell-derived exosome internalization on CD169, a transmembrane Siglec family member expressed on leukocytes and stromal cells.…”

Section: Lectinsmentioning

confidence: 99%

Internalization of Exosomes through Receptor-Mediated Endocytosis

Gonda

Kabagwira

Senthil

et al. 2019

Molecular Cancer Research

221

157

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The tumor microenvironment is replete with factors secreted and internalized by surrounding cells. Exosomes are nanosized, protein-embedded, membrane-bound vesicles that are released in greater quantities from cancer than normal cells and taken up by a variety of cell types. These vesicles contain proteins and genetic material from the cell of origin and in the case of tumor-derived exosomes, oncoproteins and oncogenes. With increasing understanding of the role exosomes play in basic biology, a more clear view of the potential exosomes are seen to have in cancer therapeutics emerges. However, certain essential aspects of exosome function, such as the uptake mechanisms, are still unknown. Various methods of cell-exosome interaction have been proposed, but this review focuses on the protein-protein interactions that facilitate receptor-mediated endocytosis, a broadly used mechanism by a variety of cells.

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“…Modern publications that employ lectin microarrays should build upon these studies by not only profiling EV glycosylation but also probing the functional roles of the glycans, as exemplified by potentially impactful research from Shimoda et al [42]. Therein, exosomes produced by human adipose derived MSCs were applied to lectin microarrays exosomes alongside purified membranes from these cells.…”

Section: Ev Glycomicsmentioning

confidence: 99%

Glycosylation of extracellular vesicles: current knowledge, tools and clinical perspectives

Williams

Royo

Aizpurua‐Olaizola

et al. 2018

J of Extracellular Vesicle

206

163

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It is now acknowledged that extracellular vesicles (EVs) are important effectors in a vast number of biological processes through intercellular transfer of biomolecules. Increasing research efforts in the EV field have yielded an appreciation for the potential role of glycans in EV function. Indeed, recent reports show that the presence of glycoconjugates is involved in EV biogenesis, in cellular recognition and in the efficient uptake of EVs by recipient cells. It is clear that a full understanding of EV biology will require researchers to focus also on EV glycosylation through glycomics approaches. This review outlines the major glycomics techniques that have been applied to EVs in the context of the recent findings. Beyond understanding the mechanisms by which EVs mediate their physiological functions, glycosylation also provides opportunities by which to engineer EVs for therapeutic and diagnostic purposes. Studies characterising the glycan composition of EVs have highlighted glycome changes in various disease states, thus indicating potential for EV glycans as diagnostic markers. Meanwhile, glycans have been targeted as molecular handles for affinity-based isolation in both research and clinical contexts. An overview of current strategies to exploit EV glycosylation and a discussion of the implications of recent findings for the burgeoning EV industry follows the below review of glycomics and its application to EV biology.

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Glycan profiling analysis using evanescent-field fluorescence-assisted lectin array: Importance of sugar recognition for cellular uptake of exosomes from mesenchymal stem cells

Cited by 116 publications

References 39 publications

Extracellular Vesicles Derived From Mesenchymal Stem Cells (MSC) in Regenerative Medicine: Applications in Skin Wound Healing

Extracellular Vesicles Derived From Mesenchymal Stem Cells (MSC) in Regenerative Medicine: Applications in Skin Wound Healing

Internalization of Exosomes through Receptor-Mediated Endocytosis

Glycosylation of extracellular vesicles: current knowledge, tools and clinical perspectives

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