Glycerol methacrylate-based copolymers: Reactivity ratios, physicochemical characterization and cytotoxicity

Automated Parallel Dialysis for Purification of Polymers

“…In contrast, a noticeable increase in the M n and Ð values (Table 2) and modification of the molar mass distribution towards higher molar mass values (Figure 4) were observed for polymer P2. This observation may be ascribed to the rise of possible interactions between P(GMMA) polymer chains via hydrogen bonding during the dialysis process, as suggested elsewhere [43], which may promote the formation of polymer species of different molar mass and, therefore, the modification of the hydrodynamic volume of such polymer chains during the respective SEC analyses. This behavior can also be related to the presence of water traces (in our case, most likely derived from the dialysis process), which may remain in the respective polymer samples, as suggested elsewhere [45,46].…”

“…The NH is connected to a solvent reservoir bottle for needle rinsing after each liquid transfer. Further details about the characteristics and capabilities of the Chemspeed ASW 2000 platform can be found elsewhere [42][43][44]. For the proposed APD method (Figure 2), a custom-made rack (Figure 2D) was employed to house the utilized and commercially available Float-A-Lyzer ®® G2 dialysis tubes (Figure 2C).…”

“…Sample dilutions and/or transfers of polymerization mixtures, for instance, from the reaction vessels into the dialysis tubes, can be automatically performed with the aid of the liquid handling system of the robotic platform. It is also possible to automatically and periodically monitor the progress of the performed chemical reaction and/or dialysis purifications at different time intervals by withdrawing aliquots from the reaction vessels and/or dialysis tubes followed by suitable analyses (e.g., 1 H-NMR or SEC measurements); the sample preparation for such an analysis can also be performed in an unattended fashion [42][43][44].…”

Multi-angle evaluation of kinetic Monte-Carlo simulations as tool to evaluate the distributed monomer composition in gradient copolymer synthesis

“…[14][15][16][17] Interestingly, amphiphilic copolymer structures can be prepared in both gradient and block configuration with one monomer unit being hydrophilic and the other hydrophobic so that the hydrodynamic properties change along the chain. [18][19][20][21][22][23] This leads to a unique capability of self-assembly in solutions as a response to external stimuli such as pH, 24,25 temperature [26][27][28] or the nature of the solvent. 22,23 Due to this behavior, amphiphilic copolymers can be found in several biomedical applications including tissue engineering, drug delivery, carriers for gene therapy, and cell encapsulation.…”