Glycine effects on in vitro motor pattern generation in mouse spinal cord

Dröge, Michael; Tao, Yuhui

doi:10.1016/0304-3940(93)90248-j

Cited by 16 publications

(10 citation statements)

References 12 publications

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“…4 A, bursts 3 and 4, B,C). A few preparations (n ϭ 3) exhibited rhythmic, mainly bilateral discharges after Ͼ2 hr of strychnine exposure; this activity superficially resembled the strychnine-induced bilateral motor activity that has been observed in neonatal mouse (Droge and Tao, 1993) and rat (Cowley and Schmidt, 1995) spinal cords in vitro.…”

Section: Strychnine-induced Paroxysmal (Seizure) Activitymentioning

confidence: 54%

Glycinergic Inhibition Contributes to the Generation of Rostral Scratch Motor Patterns in the Turtle Spinal Cord

Currie

Lee

1997

J. Neurosci.

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Cutaneous stimulation within the rostral scratch receptive field in a low spinal-immobilized turtle elicits a fictive rostral scratch reflex characterized by robust rhythmic motor output from ipsilateral hindlimb muscle nerves and weaker, alternating motor discharge in contralateral nerves. Simultaneous bilateral stimulation elicits bilateral rostral scratch motor patterns in which activity on the right and left sides alternates.We investigated the role of glycinergic inhibition in the generation and coordination of fictive rostral scratch motor patterns. Glycine (2 or 5 mM) and strychnine (5-50 M), a glycine antagonist, were superfused over the anterior spinal hindlimb enlargement while fictive rostral scratch motor output was recorded bilaterally from hindlimb muscle nerves in the form of electroneurograms (ENGs). Although glycine reduced rostral scratch burst frequencies, strychnine tended to increase burst frequency. Strychnine also changed the shape of hip flexor ENG bursts, resulting in more abrupt burst onsets, indicating an earlier recruitment of motor neurons with large ENG spikes. During bilateral stimulation, strychnine increased the variability of interlimb phase values (left vs right hip flexor bursts) but did not abolish right-left alternation.These results indicate that glycinergic neurons in or near the anterior hindlimb enlargement contribute to the overall timing of the rostral scratch rhythm and to the recruitment timing of individual hip flexor motor neurons within each scratch burst. Our data also indicate that glycinergic mechanisms contribute to, but are not critically important for, maintaining an alternating interlimb coordination during bilateral scratch motor patterns.

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Section: Strychnine-induced Paroxysmal (Seizure) Activitymentioning

confidence: 54%

Glycinergic Inhibition Contributes to the Generation of Rostral Scratch Motor Patterns in the Turtle Spinal Cord

Currie

Lee

1997

J. Neurosci.

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“…With the "functional mature" mouse spinal cord preparation at hand, the effects of nifedipine, a L-type calcium channel blocker, on a spinal motor output in animals of different age groups can be studied. Bath application of strychnine has been s h o w to produce synchronous rnotor bursting in many preparations including famprey (Harris-Warrick, l984), cat (Noga, 1993), neonatai rat (Cowley and SchmidtJ995;Braci et al, 1997) and neonatal moue (Droge and Tao, 1993). This strychnine induced synchronous activity could also be observed in the "functionally mature mice" (Jiang et al, 1999).…”

Section: Post-natal Development Of Spinal Motor Network In Mammalsmentioning

confidence: 99%

An in vitro functionally mature mouse spinal cord preparation for the study of spinal motor networks

1999

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“…As a recent report has indicated that depression of synaptic transmission by DHPG requires glycine receptors , glycinergic interneurons are also potentially involved in the effects of DHPG on ®ctive locomotion. However, this possibility could not be tested directly with a glycine antagonist because blocking glycine receptors fully abolishes ®ctive locomotion (Droge & Tao, 1993;Cazalets et al, 1994;Cowley & Schmidt, 1995).…”

Section: Group I Metabotropic Glutamate Receptors and Fictive Locomotionmentioning

confidence: 99%

Modulation of rhythmic patterns and cumulative depolarization by group I metabotropic glutamate receptors in the neonatal rat spinal cord in vitro

Taccola¹,

Marchetti

Nistri

2004

Eur J of Neuroscience

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The role of group I metabotropic glutamate receptors (mGluRs), and their subtypes 1 or 5, in rhythmic patterns generated by the neonatal rat spinal cord was investigated. Fictive locomotor patterns induced by N-methyl-d-aspartate + serotonin were slowed down by the subtype 1 antagonists (RS)-1-aminoindan-1,5-dicarboxylic acid (AIDA) or 7-(hydroxyimino)cyclopropa[b]chromen-1a-carboxylate ethyl ester (CPCCOEt) and unaffected by the subtype 5 antagonist 2-methyl-6-(phenylethynyl)pyridine hydrochloride (MPEP). The group I agonist (RS)-3,5-dihydroxyphenylglycine (DHPG) depolarized ventral roots and disrupted fictive locomotion, an effect blocked by AIDA (or CPCCOEt) and reversed by increasing the N-methyl-d-aspartate concentration. Cumulative depolarization induced by low frequency trains of dorsal root stimuli was attenuated by DHPG and unchanged by AIDA or MPEP while rhythmic patterns or motoneuron spike wind-up persisted. Disinhibited bursting induced by strychnine + bicuculline was accelerated by DHPG, slowed down by AIDA (which prevented the action of DHPG), unaffected by MPEP and counteracted by the selective group II agonist (2S,2'R,3'R)-2-(2',3'-dicarboxycyclopropyl)glycine. The DHPG transformed regular bursting into arrhythmic bursting, a phenomenon also produced by the group II mGluR antagonist (2S)-alpha-ethylglutamic acid. These results indicate that, during fictive locomotion or disinhibited bursting, endogenous glutamate could activate discrete clusters of subtype 1 mGluRs to facilitate discharges. Diffuse activation by the exogenous agonist DHPG of group I mGluRs throughout spinal networks had an excitatory effect overshadowed by its much stronger depressant action due to concomitant facilitation of glycinergic transmission. Irregular disinhibited bursting caused by activation of subtype 1 receptors or block of group II receptors suggests that mGluRs could control not only the frequency but also the periodicity of bursting patterns, outlining novel mechanisms contributing to burst duration.

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Glycine effects on in vitro motor pattern generation in mouse spinal cord

Cited by 16 publications

References 12 publications

Glycinergic Inhibition Contributes to the Generation of Rostral Scratch Motor Patterns in the Turtle Spinal Cord

Glycinergic Inhibition Contributes to the Generation of Rostral Scratch Motor Patterns in the Turtle Spinal Cord

An in vitro functionally mature mouse spinal cord preparation for the study of spinal motor networks

Modulation of rhythmic patterns and cumulative depolarization by group I metabotropic glutamate receptors in the neonatal rat spinal cord in vitro

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