GLYCINEBETAINE FROM SUGAR BEET ENHANCES THE YIELD OF Âfield-GROWNâ TOMATOES

Jokinen, Kari; Somersalo, Susanne; Mäkelä, Pirjo; Urbano, Paulo; Rojo, CÃ©sar Moreno; Gonzalez, Javier M.; Soler, Juliana; Usano, M. C.; Moure, Jesus Santiago; Moya, M.

doi:10.17660/actahortic.1999.487.33

Cited by 9 publications

(8 citation statements)

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“…, 2003). This is in addition to its known protective role against salt and drought stresses (Jokinen et al. , 1999; Mäkelä et al.…”

Section: Discussionmentioning

confidence: 97%

“…, 1996) results in increased tolerance to both salt and drought (Mäkelä et al. , 1999) and higher yields in the greenhouse and field (Jokinen et al. , 1999; Mäkelä et al.…”

Section: Introductionmentioning

confidence: 99%

“…First, its accumulation is induced under stress conditions, with levels being correlated with the extent of increased tolerance (Rhodes and Hanson, 1993). Secondly, exogenous application of GB improves the growth and survival of numerous species under stress Allard et al, 1998;Chen et al, 2000;Jokinen et al, 1999;Mä kelä et al, 1998Mä kelä et al, , 1999Zhao et al, 1992). Thirdly, GB effectively stabilizes the quaternary structures of enzymes and complex proteins, and maintains a highly ordered state of membranes when in vitro temperatures or salt concentrations are extreme (Papageorgiou and Murata, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Although tomato plants do not normally accumulate GB (Wyn Jones and Storey, 1981), exogenous GB is taken up readily when applied to their leaves. Rapid translocation to other parts of the plant (Mä kelä et al, 1996) results in increased tolerance to both salt and drought and higher yields in the greenhouse and field (Jokinen et al, 1999;Mä kelä et al, 1998). Therefore, introducing a biosynthetic GB pathway into plants might enhance their tolerance to chilling without requiring exogenous GB applications.…”

Section: Introductionmentioning

confidence: 99%

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Genetic engineering of glycinebetaine synthesis in tomato protects seeds, plants, and flowers from chilling damage

et al. 2004

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Tomato (Lycopersicon esculentum Mill.) plants, which normally do not accumulate glycinebetaine (GB), are susceptible to chilling stress. Exposure to temperatures below 10 degrees C causes various injuries and greatly decreases fruit set in most cultivars. We have transformed tomato (cv. Moneymaker) with a chloroplast-targeted codA gene of Arthrobacter globiformis, which encodes choline oxidase to catalyze the conversion of choline to GB. These transgenic plants express codA and synthesize choline oxidase, while accumulating GB in their leaves and reproductive organs up to 0.3 and 1.2 micromol g(-1) fresh weight (FW), respectively. Their chloroplasts contain up to 86% of total leaf GB. Over various developmental phases, from seed germination to fruit production, these GB-accumulating plants are more tolerant of chilling stress than their wild-type counterparts. During reproduction, they yield, on average, 10-30% more fruit following chilling stress. Endogenous GB contents as low as 0.1 micromol g(-1) FW are apparently sufficient to confer high levels of tolerance in tomato plants, as achieved via transformation with the codA gene. Exogenous application of either GB or H2O2 improves both chilling and oxidative tolerance concomitant with enhanced catalase activity. These moderately increased levels of H2O2 in codA transgenic plants, as a byproduct of choline oxidase-catalyzed GB synthesis, might activate the H2O2-inducible protective mechanism, resulting in improved chilling and oxidative tolerances in GB-accumulating codA transgenic plants. Thus, introducing the biosynthetic pathway of GB into tomato through metabolic engineering is an effective strategy for improving chilling tolerance.

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“…, 2003). This is in addition to its known protective role against salt and drought stresses (Jokinen et al. , 1999; Mäkelä et al.…”

Section: Discussionmentioning

confidence: 97%

“…, 1996) results in increased tolerance to both salt and drought (Mäkelä et al. , 1999) and higher yields in the greenhouse and field (Jokinen et al. , 1999; Mäkelä et al.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic engineering of glycinebetaine synthesis in tomato protects seeds, plants, and flowers from chilling damage

et al. 2004

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show abstract

“…In vitro assays indicate that GB acts as an osmoprotector, stabilizing both the quaternary structure of proteins and the highly ordered membrane structure under adverse conditions (Gorham, www.intechopen.com 1995). Based on the correlation between GB accumulation and stress tolerance, progress in exogenous GB application (Jokinen et al, 1999), cloning and expression of GB encoding enzymes has been achieved (Sakamoto & Murata, 2002;Quan et al, 2004). Examples of these genes include CMO (Tabuchi et al, 2005), BADH (Wood et al, 1996); CDH and BADH (Landfald & Strøm, 1986), which were cloned from different organisms and introduced into transgenic plants.…”

Section: Glycine Betainementioning

confidence: 99%