Glycome mapping on DNA sequencing equipment

Laroy, Wouter; Contreras, Roland; Callewaert, Nico

doi:10.1038/nprot.2006.60

Cited by 171 publications

(202 citation statements)

References 23 publications

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“…Our department developed an ultra-sensitive technique to quantitatively profile and sequence N-linked glycans using standard DNA-sequencing equipment (Laroy et al, 2006), and we adapted this technique for the study of human ageing (Vanhooren et al, 2009). Using "DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis" (DSA-FACE), an accurate fingerprint of the glycan composition in serum or plasma can be obtained.…”

Section: The Healthy Human Ageing Biomarker Glycoagetest: Shift Of Thmentioning

confidence: 99%

Serum N-glycan profile shift during human ageing

Vanhooren

Dewaele

Libert

et al. 2010

Experimental Gerontology

112

100

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Section: The Healthy Human Ageing Biomarker Glycoagetest: Shift Of Thmentioning

confidence: 99%

Serum N-glycan profile shift during human ageing

Vanhooren

Dewaele

Libert

et al. 2010

Experimental Gerontology

112

100

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“…We have explored the diagnostic possibilities of Hpx N-glycosylation changes as a marker for HCC, using the sensitive DSA-FACE technique (10,11 ).…”

Section: Hepatocellular Carcinoma (Hcc)mentioning

confidence: 99%

Diagnostic Value of the Hemopexin N-Glycan Profile in Hepatocellular Carcinoma Patients

et al. 2010

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BACKGROUND: Hepatocellular carcinoma (HCC) is a common and rapidly fatal cancer. Current diagnostic methods for HCC have poor sensitivity and specificity, are invasive, and carry risk for complications. Newer markers are needed to overcome these problems and allow diagnosis of HCC at an earlier stage. In view of known associations between glycosylation changes and liver disease, we focused on the serum glycoprotein hemopexin and the specific characteristics of this liversynthesized glycoprotein.

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“…The N-glycosylation profile of the 96-DWP eluates were analyzed by capillary gel electrophoresis with laser-induced fluorescence detection (CGE-LIF, DNA genetic analyzer 3130XL, Life Technologies, Darmstadt, Germany) 211 . The neutralized ST and 3.5-L bioreactor samples were denatured by IAA in 0.6 M denaturation reagent (GlykoPrep-plus, Europa Bioproducts, Cambridge, UK) and reduced.…”

Section: Glycan Analysismentioning

confidence: 99%

Tailoring recombinant protein quality by rational media design

Brühlmann

Jordan

Hemberger³

et al. 2015

Biotechnology Progress

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Clinical efficacy and safety of recombinant proteins are closely associated with their structural characteristics. The major quality attributes comprise glycosylation, charge variants (oxidation, deamidation, and C‐ & N‐terminal modifications), aggregates, low‐molecular‐weight species (LMW), and misincorporation of amino acids in the protein backbone. Cell culture media design has a great potential to modulate these quality attributes due to the vital role of medium in mammalian cell culture. The purpose of this review is to provide an overview of the way both classical cell culture medium components and novel supplements affect the quality attributes of recombinant therapeutic proteins expressed in mammalian hosts, allowing rational and high‐throughput optimization of mammalian cell culture media. A selection of specific and/or potent inhibitors and activators of oligosaccharide processing as well as components affecting multiple quality attributes are presented. Extensive research efforts in this field show the feasibility of quality engineering through media design, allowing to significantly modulate the protein function. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 31:615–629, 2015

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Glycome mapping on DNA sequencing equipment

Cited by 171 publications

References 23 publications

Serum N-glycan profile shift during human ageing

Serum N-glycan profile shift during human ageing

Diagnostic Value of the Hemopexin N-Glycan Profile in Hepatocellular Carcinoma Patients

Tailoring recombinant protein quality by rational media design

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