Glycoprotein D peptide-based diagnostic approach for the detection of avian infectious laryngotracheitis antibodies

Kumar, Vishnu; Yadav, K.S.; Kumar, Rakesh; Chaudhary, Nitin; Kumar, Sachin

doi:10.1080/03079457.2019.1631444

Cited by 8 publications

(5 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, glycoprotein D (gD) based ELISA has been developed where two immunogenic regions were identified and synthesized. This synthetic peptide was used in the developed ELISA which showed sensitivity of 96.9% and specificity of 87.5% (Kumar et al 2019).…”

Section: Diagnosismentioning

confidence: 99%

Infectious laryngotracheitis: Etiology, epidemiology, pathobiology, and advances in diagnosis and control – a comprehensive review

Gowthaman

Kumar

Koul

et al. 2020

Veterinary Quarterly

Self Cite

View full text Add to dashboard Cite

Infectious laryngotracheitis (ILT) is a highly contagious upper respiratory tract disease of chicken caused by a Gallid herpesvirus 1 (GaHV-1) belonging to the genus Iltovirus, and subfamily Alphaherpesvirinae within Herpesviridae family. The disease is characterized by conjunctivitis, sinusitis, oculo-nasal discharge, respiratory distress, bloody mucus, swollen orbital sinuses, high morbidity, considerable mortality and decreased egg production. It is well established in highly dense poultry producing areas of the world due to characteristic latency and carrier status of the virus. Co-infections with other respiratory pathogens and environmental factors adversely affect the respiratory system and prolong the course of the disease. Latently infected chickens are the primary source of ILT virus (ILTV) outbreaks irrespective of vaccination. Apart from conventional diagnostic methods including isolation and identification of ILTV, serological detection, advanced biotechnological tools such as PCR, quantitative real-time PCR, next generation sequencing, and others are being used in accurate diagnosis and epidemiological studies of ILTV. Vaccination is followed with the use of conventional vaccines including modified live attenuated ILTV vaccines, and advanced recombinant vector vaccines expressing different ILTV glycoproteins, but still these candidates frequently fail to reduce challenge virus shedding. Some herbal components have proved to be beneficial in reducing the severity of the clinical disease. The present review discusses ILT with respect to its current status, virus characteristics, epidemiology, transmission, pathobiology, and advances in diagnosis, vaccination and control strategies to counter this important disease of poultry.

show abstract

Section: Diagnosismentioning

confidence: 99%

Infectious laryngotracheitis: Etiology, epidemiology, pathobiology, and advances in diagnosis and control – a comprehensive review

Gowthaman

Kumar

Koul

et al. 2020

Veterinary Quarterly

Self Cite

View full text Add to dashboard Cite

show abstract

“…Among those eight proteins, gD, gG, gI, and gJ were glycoproteins that were thought to be important for ILTV replication and eliciting humoral and cell-mediated immune responses in the host [29]. Previous studies have found that gD binds to the susceptible host cell receptor [34,35], gG plays an immunomodulatory role in the chicken immune response [10,36], gI plays a role in virus cell-to-cell spread by forming a heterodimer with gE [37], and gJ plays a role in virus release [38]. Whether the amino acid mutation in gD, gG, gI, and gJ of ILTVs leads to the replication and pathogenicity differences needs further study.…”

Section: Discussionmentioning

confidence: 99%

Biological Characteristics of Infectious Laryngotracheitis Viruses Isolated in China

Zhang

et al. 2022

Viruses

View full text Add to dashboard Cite

Infectious laryngotracheitis virus (ILTV) causes severe respiratory disease in chickens and results in huge economic losses in the poultry industry worldwide. To correlate the genomic difference with the replication and pathogenicity, phenotypes of three ILTVs isolated from chickens in China from 2016 to 2018 were sequenced by high-throughput sequencing. Based on the entire genome, the isolates GD2018 and SH2017 shared 99.9% nucleotide homology, while the isolate SH2016 shared 99.7% nucleotide homology with GD2018 and SH2017, respectively. Each virus genome contained 82 ORFs encoding 77 kinds of protein, 31 of which share the same amino acid sequence in the three viruses. GD2018 and SH2017 shared 57 proteins with the same amino acid sequence, while SH2016 shared 42 and 41 proteins with the amino acid sequences of GD2018 and SH2017, respectively. SH2016 propagated efficiently in allantoic fluid and on chorioallantoic membranes (CAMs) of SPF chicken embryo eggs, while GD2018 and SH2017 proliferated well only on CAMs. GD2018 propagated most efficiently on CAMs and LMH cells among three isolates. SH2016 caused serious clinical symptoms, while GD2018 and SH2017 caused mild and moderate clinical symptoms in chickens, although the sero of the chickens infected with those three isolates were all positive for anti-ILTV antibody at 14 and 21 days after challenge. Three ILTVs with high genetic homology showed significant differences in the replication in different culture systems and the pathogenicity of chickens, providing basic materials for studying the key determinants of pathogenicity of ILTV.

show abstract

“…Antibodies to ILTV were detected by enzyme-linked immunosorbent assay as previously described [25]. The serum samples from immunized chickens were collected at 7, 14, and 21 days after vaccination.…”

Section: Protective Efficacy Of Rfpv-gb Against Iltvs In Spf Chickensmentioning

confidence: 99%

Recombinant Fowlpox Virus Expressing gB Gene from Predominantly Epidemic Infectious Larygnotracheitis Virus Strain Demonstrates Better Immune Protection in SPF Chickens

Chen

et al. 2020

Vaccines

View full text Add to dashboard Cite

Background: Infectious laryngotracheitis (ILT) is a highly contagious acute respiratory disease of chickens. Antigenic mutation of infectious laryngotracheitis virus (ILTV) may result in a vaccination failure in the poultry industry and thus a protective vaccine against predominant ILTV strains is highly desirable. Methods: The full-length glycoprotein B (gB) gene of ILTV with the two mutated synonymous sites of fowlpox virus (FPV) transcription termination signal sequence was cloned into the insertion vector p12LS, which was co-transfected with wild-type (wt) FPV into chicken embryo fibroblast (CEF) to develop a recombinant fowlpox virus-gB (rFPV-gB) candidate vaccine strain. Furthermore, its biological and immunological characteristics were evaluated. Results: The results indicated that gB gene was expressed correctly in the rFPV by indirect immunofluorescent assay and Western blot, and the rFPV-gB provided a 100% protection in immunized chickens against the challenge of predominant ILTV strains that were screened by pathogenicity assay when compared with the commercialized rFPV vaccine, which only provided 83.3%. Conclusion: rFPV-gB can be used as a potential vaccine against predominant ILTV strains.

show abstract

Glycoprotein D peptide-based diagnostic approach for the detection of avian infectious laryngotracheitis antibodies

Cited by 8 publications

References 53 publications

Infectious laryngotracheitis: Etiology, epidemiology, pathobiology, and advances in diagnosis and control – a comprehensive review

Infectious laryngotracheitis: Etiology, epidemiology, pathobiology, and advances in diagnosis and control – a comprehensive review

Biological Characteristics of Infectious Laryngotracheitis Viruses Isolated in China

Recombinant Fowlpox Virus Expressing gB Gene from Predominantly Epidemic Infectious Larygnotracheitis Virus Strain Demonstrates Better Immune Protection in SPF Chickens

Contact Info

Product

Resources

About