Glycosylation pattern and axin expression in normal and IUGR placentae

Vukasovic, Andreja; Grbeša, Đurđica; Martić, Tamara Nikuševa; Kušec, Vesna; Mišković, Berivoj; Šerman, Alan; Soken, Nikolina; Šerman, Ljiljana

doi:10.3109/14767058.2014.926326

Cited by 4 publications

(1 citation statement)

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“…This study aimed to determine the expression of Dishevelled proteins and their mRNA in healthy placentas and placentas with intrauterine growth restriction (IUGR). Since the placenta needs to continually adapt during its development to the demands placed on it by the growing embryo/fetus, the cells that contribute most to this adaptability, the trophoblast cells, must be under constant control a series of molecular mechanisms [ 13 ]. One such mechanism is the Wnt signaling pathway, whose central mediators are the DVL proteins.…”

Section: Discussionmentioning

confidence: 99%

Dishevelled family proteins (DVL1-3) expression in IUGR placentas

Sola

Šerman²,

Karin-Kujundžić

et al. 2020

Bosn J of Basic Med Sci

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Dishevelled family proteins (DVL1, DVL2, and DVL3) are cytoplasmic mediators involved in canonical and non-canonical Wnt signaling that are important in embryonic development. The role of DVL proteins in the placental tissue remains mostly unknown. In the current study, we explored the role of Dishevelled proteins in naturally invasive tissue, trophoblast. Formalin-fixed paraffin-embedded samples of 15 term placentas from physiologic term pregnancies and 15 term placentas from pregnancies complicated with intrauterine growth restrictions (IUGR) were used for the study. Expression levels of mRNA for DVL1, DVL2, and DVL3 in placentas were analyzed by quantitative real-time PCR (qRT-PCR). DVL1, DVL2, and DVL3 protein expression were semi-quantitatively analyzed using immunohistochemistry. The expression of DVL2 and DVL3 proteins was significantly higher in trophoblasts in placental villi from IUGR pregnancies compared with the control group of term placentas. In contrast, DVL3 protein expression was significantly higher in endothelial cells in placental villi from IUGR pregnancies compared with normal term placentas. The observed differences at protein levels between normal and IUGR placentas were not confirmed at the mRNA levels of DVL genes. Our data indicate the active involvement of DVL proteins in IUGR-related placentas. No significant changes were observed in DVL mRNA levels between the two groups of placentas. Further studies are required to explore the clinical relevance of these observations.

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Section: Discussionmentioning

confidence: 99%