Glycovaccinology: The design and engineering of carbohydrate-based vaccine components

Hulbert, Sophia W.; Desai, Primit; Jewett, Michael C.; DeLisa, Matthew P.; Williams, Asher J.

doi:10.1016/j.biotechadv.2023.108234

Cited by 5 publications

(1 citation statement)

References 234 publications

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“…Vaccines are one of the most important achievements in preventive medicine because they ensure protection against a broad range of infections by stimulating humoral and cellular immune responses. Carbohydrates, found abundantly on the surface of many pathogens, are attractive candidates for vaccine development due to their interaction with both innate and adaptive immunity [1][2][3]. Many licensed vaccines use bacterial cell-surface antigens conjugated to subunit proteins to trigger antigen-specific protective antibodies and long-lasting immune responses by eliciting T-cell responses and memory B cell activation [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Impact of Protein Nanoparticle Shape on the Immunogenicity of Antimicrobial Glycoconjugate Vaccines

Dolce,

Proietti,

Principato

et al. 2024

IJMS

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Protein self-assembling nanoparticles (NPs) can be used as carriers for antigen delivery to increase vaccine immunogenicity. NPs mimic the majority of invading pathogens, inducing a robust adaptive immune response and long-lasting protective immunity. In this context, we investigated the potential of NPs of different sizes and shapes—ring-, rod-like, and spherical particles—as carriers for bacterial oligosaccharides by evaluating in murine models the role of these parameters on the immune response. Oligosaccharides from Neisseria meningitidis type W capsular polysaccharide were conjugated to ring-shape or nanotubes of engineered Pseudomonas aeruginosa Hemolysin-corregulated protein 1 (Hcp1cc) and to spherical Helicobacter pylori ferritin. Glycoconjugated NPs were characterized using advanced technologies such as High-Performance Liquid Chromatography (HPLC), Asymmetric Flow-Field Flow fractionation (AF4), and Transmission electron microscopy (TEM) to verify their correct assembly, dimensions, and glycosylation degrees. Our results showed that spherical ferritin was able to induce the highest immune response in mice against the saccharide antigen compared to the other glycoconjugate NPs, with increased bactericidal activity compared to benchmark MenW-CRM197. We conclude that shape is a key attribute over size to be considered for glycoconjugate vaccine development.

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Section: Introductionmentioning

confidence: 99%

Impact of Protein Nanoparticle Shape on the Immunogenicity of Antimicrobial Glycoconjugate Vaccines

Dolce,

Proietti,

Principato

et al. 2024

IJMS

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Semisynthetic Glycoconjugates as Potential Vaccine Candidates Against Haemophilus influenzae Type a

Kohout,

Del Bino,

Petrosilli

et al. 2024

Chemistry A European J

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Glycoconjugate vaccines are based on chemical conjugation of pathogen‐associated carbohydrates with immunogenic carrier proteins and are considered a very cost‐effective way to prevent infections. Most of the licensed glycoconjugate vaccines are composed of saccharide antigens extracted from bacterial sources. However, synthetic oligosaccharide antigens have become a promising alternative to natural polysaccharides with the advantage of being well‐defined structures providing homogeneous conjugates. Haemophilus influenzae (Hi) is responsible for a number of severe diseases. In recent years, an increasing rate of invasive infections caused by Hi serotype a (Hia) raised some concern, because no vaccine targeting Hia is currently available. The capsular polysaccharide (CPS) of Hia is constituted by phosphodiester‐linked 4‐β‐d‐glucose‐(1→4)‐d‐ribitol‐5‐(PO4→) repeating units and is the antigen for protein‐conjugated polysaccharide vaccines. To investigate the antigenic potential of the CPS from Hia, we synthesized related saccharide fragments containing up to five repeating units. Following the synthetic optimization of the needed disaccharide building blocks, they were assembled using the phosphoramidite approach for the installation of the phosphodiester linkages. The resulting CPS‐based Hia oligomers were conjugated to CRM197 carrier protein and evaluated in vivo for their immunogenic potential, showing that all glycoconjugates were capable of raising antibodies recognizing Hia synthetic fragments.

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Gastrointestinal microbiome engineering in pig

Veerapagu,

Jeya,

Sankara Narayanan

2025

Human and Animal Microbiome Engineering

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Glycovaccinology: The design and engineering of carbohydrate-based vaccine components

Cited by 5 publications

References 234 publications

Impact of Protein Nanoparticle Shape on the Immunogenicity of Antimicrobial Glycoconjugate Vaccines

Impact of Protein Nanoparticle Shape on the Immunogenicity of Antimicrobial Glycoconjugate Vaccines

Semisynthetic Glycoconjugates as Potential Vaccine Candidates Against Haemophilus influenzae Type a

Gastrointestinal microbiome engineering in pig

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