Glyphosate induced toxicity to chickpea plants and stress alleviation by herbicide tolerant phosphate solubilizing Burkholderia cepacia PSBB1 carrying multifarious plant growth promoting activities

Abstract

In this study, strain PSBB1 isolated from rhizosphere was identified as, by 16S rDNA sequence analysis and characterized. Strain PSBB1 tolerated glyphosate up to 3200 μg ml and produced IAA (81.6 μg ml), ACC deaminase (69.3 mg protein h), SA (39.3 μg ml) and 2,3-DHBA (26.6 μg ml), solubilized insoluble P (50.8 μg ml) and secreted 29.4 μg ml exopolysaccharides, which decreased with increasing concentrations of glyphosate. Cell damage following glyphosate application was visible under SEM and CLSM. The phytotoxicity of glyphosate on chickpea was variable but significant. . mitigated toxicity and enhanced the size, dry matter, symbiosis, seed attributes and nutritional contents of chickpea. Further, strain PSBB1 declined the levels of CAT, POD, APX and GPX and MDA contents at 4332 μg kg soil glyphosate. Proline also increased under glyphosate stress but declined in inoculated plants. The ability to tolerate higher concentration of glyphosate, the capacity to secrete plant growth regulators even under herbicide stress and potential to reduce the level of proline and antioxidant enzymes makes as an interesting choice for enhancing chickpea production in soils contaminated even with herbicides.

Fungicide-Tolerant Plant Growth-Promoting Rhizobacteria Mitigate Physiological Disruption of White Radish Caused by Fungicides Used in the Field Cultivation

“…Hence, release of siderophores under Fe-limited conditions could be useful, as siderophore-producing PGPR isolates may be applied for the bio-management of plant pathogens [ 58 , 59 ]. Production of siderophores by soil microorganisms in stressed environments has previously been reported [ 41 , 60 , 61 ].…”

“…Fungicide-induced changes in integrity of bacterial membrane were assessed using CLSM following the method of Shahid and Khan [ 41 ]. A total of 200 µL fungicide-treated or untreated suspensions of freshly grown bacteria and 10 µL propidium iodide [PI: 50 μg mL −1 prepared in phosphate buffer saline (PBS)] dye was mixed.…”

Toxicity of fungicides toPisum sativum: a study of oxidative damage, growth suppression, cellular death and morpho-anatomical changes

“…In a similar study, a concentration-dependent increase in proline content in various organs of Cicer arietinum plants grown under a herbicide-stressed environment has recently been reported. 44 Since, lipid peroxidation is believed to act as a biomarker for oxidative stress, the assessment of MDA content in plants is inevitable. Malondialdehyde (MDA), synthesized in plants, is a decomposition product of the polyunsaturated fatty acid component of membrane lipid.…”

Role of halotolerant phosphate‐solubilising bacteria on growth promotion of peanut (Arachis hypogaea) under saline soil

“…Egamberdieva et al () demonstrated the colonisation potential of P. chlororaphis TSAU13 and P. extremorientalis TSAU20 strains on tomato roots under saline soil conditions. Similar results were also described by Shahid and Khan () and Shankar, Ponraj, Ilakkiam, and Gunasekaran (). It has been reported that the successful rhizosphere colonisation of PGPR is affected by a variety of stressors, such as drought, temperature, salinity and soil type (Compant, Clément, & Sessitsch, ).…”