Glyphosate Uptake by Suspension-Cultured Potato (<i>Solanum tuberosum</i>and<i>S. brevidens</i>) Cells

Burton, J. D.; Balke, Nelson E.

doi:10.1017/s0043174500074634

Cited by 20 publications

(24 citation statements)

References 32 publications

(52 reference statements)

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“…Nevertheless, the low rate of PHE uptake after the initial rapid uptake phase is mainly attributable to passage into root cytoplasm and vacuole. The time course of PHE uptake by wheat roots is consistent with that of glyphosate uptake by suspension-cultured potato [34]. Denis and Delrot [35], and Tilquin et al .…”

Section: Discussionsupporting

confidence: 54%

Accumulation of phenanthrene by roots of intact wheat (Triticum acstivnmL.) seedlings: passive or active uptake?

Zhan

Zhou

et al. 2010

BMC Plant Biol

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BackgroundPolycyclic aromatic hydrocarbons (PAHs) are of particular concern due to their hydrophobic, recalcitrant, persistent, potentially carcinogenic, mutagenic and toxic properties, and their ubiquitous occurrence in the environment. Most of the PAHs in the environment are present in surface soil. Plants grown in PAH-contaminated soils or water can become contaminated with PAHs because of their uptake. Therefore, they may threaten human and animal health. However, the mechanism for PAHs uptake by crop roots is little understood. It is important to understand exactly how PAHs are transported into the plant root system and into the human food chain, since it is beneficial in governing crop contamination by PAHs, remedying soils or waters polluted by PAHs with plants, and modeling potential uptake for risk assessment.ResultsThe possibility that plant roots may take up phenanthrene (PHE), a representative of PAHs, via active process was investigated using intact wheat (Triticum acstivnm L.) seedlings in a series of hydroponic experiments. The time course for PHE uptake into wheat roots grown in Hoagland solution containing 5.62 μM PHE for 36 h could be separated into two periods: a fast uptake process during the initial 2 h and a slow uptake component thereafter. Concentration-dependent PHE uptake was characterized by a smooth, saturable curve with an apparent Km of 23.7 μM and a Vmax of 208 nmol g-1 fresh weight h-1, suggesting a carrier-mediated uptake system. Competition between PHE and naphthalene for their uptake by the roots further supported the carrier-mediated uptake system. Low temperature and 2,4-dinitrophenol (DNP) could inhibit PHE uptake equally, indicating that metabolism plays a role in PHE uptake. The inhibitions by low temperature and DNP were strengthened with increasing concentration of PHE in external solution within PHE water solubility (7.3 μM). The contribution of active uptake to total absorption was almost 40% within PHE water solubility. PHE uptake by wheat roots caused an increase in external solution pH, implying that wheat roots take up PHE via a PHE/nH+ symport system.ConclusionIt is concluded that an active, carrier-mediated and energy-consuming influx process is involved in the uptake of PHE by plant roots.

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Section: Discussionsupporting

confidence: 54%

Accumulation of phenanthrene by roots of intact wheat (Triticum acstivnmL.) seedlings: passive or active uptake?

Zhan

Zhou

et al. 2010

BMC Plant Biol

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“…However, in the kinetics studies with a lipophilic herbicide, for example ametryn and atrazine, an equilibrium was reached very rapidly in about 10 min . In the case of methiozolin, uptake continued for a much longer period until at least 48 h; and the overall kinetic pattern over the 168 h appeared to be biphasic, which is a typical kinetics of an active absorption system involving a transporter . A calculated theoretical tissue concentration of C was much higher than the external solution (data not shown), further suggesting methiozolin absorption might not be a simple diffusion but involve an active transport system or other factors such as cell wall deposition.…”

Section: Resultsmentioning

confidence: 96%

Methiozolin [5-(2,6-difluorobenzyl)oxymethyl-5-methyl-3,3(3-methylthiophen-2-yl)-1,2-isoxazoline], a new annual bluegrass (Poa annuaL.) herbicide for turfgrasses

et al. 2013

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“…A foliar-applied herbicide must pass through several barders in order to reach its site of action. The major barrier to polar herbicides is the cuticle (Hess, 1985), but the underlying plasma membrane is also considered to be a barder to charged molecules such as glyphosate (Richard & Slife, 1979;Brecke & Duke, 1980;Burton, 1986).…”

Section: Wirkung Von Zusatzstoffen Und Temperatur Auf Die Absorption mentioning

confidence: 99%

Effects of adjuvants and temperature on glyphosate absorption by cultured cells of velvetleaf (Abutilon theophrasti Medic.)

1992

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Summary: Résumé: Zusammenfassung Effects of temperature and three adjuvants (ammonium sulphate, Armoblen T/25, and BanDrift) on absorption of glyphosate by suspension‐cultured velvetleaf (Abutilon theo phrasti) cells were examined. Velvetleaf cells absorbed glyphosate to a concentration about 40% of the external media concentration following incubation for 3 h. Ammonium sulphate at concentrations of 0.1 mM to 10.0 mM did not significantly affect glyphosate absorption. The results were similar for Armoblen T/25. However, Armoblen T/25 was toxic to the cells at concentrations as low as 0.002% (v/v). BanDrift showed no toxicity to the cells, but significantly increased glyphosate absorption. This stimulation of glyphosate absorption was present at 4°C as well as 28°C. Effets d'adjuvants et de la température surl'absorption du glyphosate par des cellules cultivées de jute de Chine (Abutilon theophrasti Medic.) Les effets de la température et de 3 adjuvants (sulfate d'ammonium, Armoblen T/25 et BanDrift) sur l'absorption du glyphosate par une culture en suspension de cellules de jute de Chine (Abutilon theophrasti) ont étéétudiées. Les cellules de jute de Chine ont absorbé le glyphosate à une concentration voisine de 40% de la concentration du milieu externe après une incubation de 3 heures. Le sulfate d'ammonium à des concentrations de 0,1 mM à 10 mM n'a pas significativement affecté l'absorption du glyphosate. Les résultats ont été similaires avec l'Armoblen T 25. Cependant, l'Armoblen T/25 était toxique pour les cellules à des concentrations aussi faibles que 0,002% (v/v). Le BanDrift n'a pas exprimé de toxicité vis‐à‐vis des cellules mais a significativement fait augmenter l'absorption du glyphosate. Cette stimulation de l'absorption du glyphosate était présente à 40 °C tout comme à 28°C. Wirkung von Zusatzstoffen und Temperatur auf die Absorption von Glyphosat in Zellkulturen der Samtpappel (Abutilon theophrasti Medic.) Die Wirkung der Temperatur und von 3 Zusatzstoffen (Ammoniumsulfat, Armoblen T/25, BanDrift) auf die Absorption von Glyphosat in Zellkultursuspensionen der Samtpappel (Abutilon theophrasti) wurde untersucht. Innerhalb 3 h absorbierten die Zellen Glyphosat bis zu 40% der Konzentration im Medium. Nach Zugabe von 0,1 bis 10,0 mM Ammoniumsulfat wurde die Glyphosatauf‐nahme nicht signifikant beeinflußt. Bei Armoblen T/25 waren die Ergebnisse ähnlich, doch war dieser Zusatzstoff schon bei 0,002 Vol.‐% toxisch. BanDrift war nicht toxisch und erhöhte die Absorption signifikant, sowohl bei 4 als auch bei 28 °C.

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Glyphosate Uptake by Suspension-Cultured Potato (Solanum tuberosumandS. brevidens) Cells

Cited by 20 publications

References 32 publications

Accumulation of phenanthrene by roots of intact wheat (Triticum acstivnmL.) seedlings: passive or active uptake?

Accumulation of phenanthrene by roots of intact wheat (Triticum acstivnmL.) seedlings: passive or active uptake?

Methiozolin [5-(2,6-difluorobenzyl)oxymethyl-5-methyl-3,3(3-methylthiophen-2-yl)-1,2-isoxazoline], a new annual bluegrass (Poa annuaL.) herbicide for turfgrasses

Effects of adjuvants and temperature on glyphosate absorption by cultured cells of velvetleaf (Abutilon theophrasti Medic.)

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