Glypican-3 electrochemical aptasensor based on reduced graphene oxide‐chitosan‐ferrocene deposition of platinum–palladium bimetallic nanoparticles

Shi, Xiaohang; Chen, Min; Feng, Huafu; Zhou, Zhide; Wu, Runqiang; Li, Wenzhan; Liang, Jianwei; Chen, Jiejing; Li, Guiyin

doi:10.1007/s10800-021-01534-4

Cited by 27 publications

(6 citation statements)

References 45 publications

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“…However, there is still concern about their toxicity for both humans and the environment [ 14 ], so most of their cancer-related applications are limited to their use in the development of sensors for cancer biomarkers. Their high surface area and excellent electrical conductivity [ 15 ] make them suitable candidates for the development of the electrochemical sensors used for the detection of biomarkers that are expressed in HCC [ 16 , 17 , 18 ].…”

Section: Types Of Nanoparticles Used For Diagnosis and Treatmentmentioning

confidence: 99%

“…Until then, AuNPs and other metallic nanoparticles remain valuable candidates for diagnostic techniques, such as the electrochemical detection of different biomarkers. In the context of HCC, gold nanoparticles [ 17 ], gold–platinum [ 16 ], or platinum–palladium nanostructures [ 18 ] have been used in conjunction with carbon-based nanomaterials, for the detection of biomarkers such as AFP or glypican-3. Gold nanostructures can, also, be employed for the imaging and photothermal ablation of tumors [ 27 ].…”

Section: Types Of Nanoparticles Used For Diagnosis and Treatmentmentioning

confidence: 99%

“…Metallic nanoparticles were, also, employed for the electrochemical detection of glypican 3. Li’s group reported two similar methods, using gold nanoparticles [ 17 ] and platinum–palladium structures [ 18 ], respectively. These nanomaterials were grafted onto electrode surfaces, together with reduced graphene oxide, hemin [ 17 ], or ferrocene [ 18 ], as redox mediators, and the glypican-3-specific aptamer, which was immobilized via cross-linking.…”

Section: Nanoparticles Used For Diagnosticsmentioning

confidence: 99%

“…Li’s group reported two similar methods, using gold nanoparticles [ 17 ] and platinum–palladium structures [ 18 ], respectively. These nanomaterials were grafted onto electrode surfaces, together with reduced graphene oxide, hemin [ 17 ], or ferrocene [ 18 ], as redox mediators, and the glypican-3-specific aptamer, which was immobilized via cross-linking. The detection was done by measuring the decrease in the differential-pulse voltammetry oxidation peak of the redox mediator, when glypican-3 was added to the aptasensor.…”

Section: Nanoparticles Used For Diagnosticsmentioning

confidence: 99%

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Nanotechnology in the Diagnostic and Therapy of Hepatocellular Carcinoma

et al. 2022

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Hepatocellular carcinoma is the most common liver malignancy and is among the top five most common cancers. Despite the progress of surgery and chemotherapy, the results are often disappointing, in part due to chemoresistance. This type of tumor has special characteristics that allow the improvement of diagnostic and treatment techniques used in clinical practice, by combining nanotechnology. This article presents a brief review of the literature focused on nano-conditioned diagnostic methods, targeted therapy, and therapeutic implications for the pathology of hepatocellular carcinoma. Within each subdomain, several modern technologies with significant impact were highlighted: serological, imaging, or histopathological diagnosis; intraoperative detection; carrier-type nano-conditioned therapy, thermal ablation, and gene therapy. The prospects offered by nanomedicine will strengthen the hope of more efficient diagnoses and therapies in the future.

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Section: Types Of Nanoparticles Used For Diagnosis and Treatmentmentioning

confidence: 99%

Section: Types Of Nanoparticles Used For Diagnosis and Treatmentmentioning

confidence: 99%

Section: Nanoparticles Used For Diagnosticsmentioning

confidence: 99%

Section: Nanoparticles Used For Diagnosticsmentioning

confidence: 99%

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Nanotechnology in the Diagnostic and Therapy of Hepatocellular Carcinoma

et al. 2022

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“…To the best of our knowledge, the use of the CNFs and chitosan (CHIT) for the fabrication of the RNA-based aptasensor to detect CRP has not been reported yet. Both CNFs and CHIT are bio-friendly materials that provide a high degree of biocompatible microenvironment for the bio-recognizer, improving the stability of the biosensor [ 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive RNA-Based Electrochemical Aptasensor for the Determination of C-Reactive Protein Using Carbon Nanofiber-Chitosan Modified Screen-Printed Electrode

Tabrizi

Acedo

2022

Nanomaterials

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C-reactive protein (CRP) is one of the biomarkers related to coronavirus disease 2019 (COVID-19). Therefore, it is crucial to develop a highly sensitive, selective, and cost-effective biosensor for the determination of CRP. In this study, we designed an electrochemical aptasensor. For this purpose, the surface of a carbon screen-printed electrode was first modified with a carbon nanofiber-chitosan (CNFs-CHIT) nanocomposite. After that, the amino-terminal RNA aptamer probes were linked to the amino groups of CHIT via glutaraldehyde as the cross-linker. Finally, methylene blue (MB) as a redox probe was self-assembled on the surface of the aptasensor. The obtained results indicated that the CNFs-CHIT nanocomposite increased the surface coverage of the aptamer up to 5.9 times. The square-wave voltammetry was used for the measurement of CRP concentration in the linear range of 1.0–150.0 pM. The obtained results indicated that the signal had a logarithmic relationship with the concentration of CRP. The limit of detection (LOD) was obtained to be 0.37 pM. The dissociation constant (Kd) that demonstrates the affinity of the aptamer probe to its target was found to be 0.93 pM. The analytical performances of the proposed RNA aptasensor were better than the previously reported aptasensors for CRP. The proposed aptasensor was also applied for the determination of CRP in the human plasma samples. The obtained results indicated that there were no statistically significant differences between the responses of the proposed RNA aptasensor and an enzyme-linked immunosorbent assay kit (ELISA). The analytical performances of the proposed RNA aptasensor described in this paper are better than previously reported aptasensors for CRP determination.

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Bioelectrochemical Sensing Using Benchtop Fabricated Nanoroughened Microstructured Electrodes

González‐Martínez,

Moran‐Mirabal

2024

Advanced Sensor Research

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Cost‐effective miniaturized electrodes that maintain a high electroactive surface area (ESA) are needed for the widespread deployment of point‐of‐care sensors. Cost‐effective methods are recently developed to fabricate nanoroughened microstructured gold electrodes (NR‐MSEs) with ultrahigh ESA. In this work, the effectiveness of NR‐MSEs for bioelectrochemical enzymatic sensors is evaluated. A glucose sensor is constructed by first casting onto NR‐MSEs a solution containing reduced graphene oxide decorated with gold nanoparticles, glucose oxidase, and glutaraldehyde, followed by a solution containing ferrocene, and a layer of chitosan to prevent the leakage of sensor components. A urea biosensor is also fabricated using Nafion as a cationic exchanger for the electropolymerization of polyaniline, followed by the deposition of a composite containing urease, bovine serum albumin, and glutaraldehyde. The limit of quantification for both biosensors is below clinically relevant concentrations of the analytes in biofluids, 0.67 mm for glucose and 1.70 mm for urea. The sensors exhibit excellent performance in complex matrixes (human blood serum and wine for glucose and human blood serum and urine for urea), with recovery for spiked analytes in the range of 92–108%. It is anticipated that NR‐MSEs will expedite the development of highly sensitive bioelectrochemical sensors for use in resource‐limited settings.

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Glypican-3 electrochemical aptasensor based on reduced graphene oxide‐chitosan‐ferrocene deposition of platinum–palladium bimetallic nanoparticles

Cited by 27 publications

References 45 publications

Nanotechnology in the Diagnostic and Therapy of Hepatocellular Carcinoma

Nanotechnology in the Diagnostic and Therapy of Hepatocellular Carcinoma

Highly Sensitive RNA-Based Electrochemical Aptasensor for the Determination of C-Reactive Protein Using Carbon Nanofiber-Chitosan Modified Screen-Printed Electrode

Bioelectrochemical Sensing Using Benchtop Fabricated Nanoroughened Microstructured Electrodes

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