Gold Coated Nanoparticles Functionalized by Photochemical Immobilization Technique for Immunosensing

Ventura, Bartolomeo Della; Campanile, Raffaele; Cimafonte, Martina; Elia, Valerio Cosimo; Forente, A.; Minopoli, Antonio; Scardapane, Emanuela; Schiattarella, Chiara; Velotta, R.

doi:10.1007/978-3-030-69551-4_16

Cited by 2 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alternatively, the specificity of AuNPs in biosensors has been used to increase the sensitivity of quartzcrystal microbalances and as optical transducers for colorimetric biosensors. For this purpose, gold-coated core-shell magnetic NPs were functionalized with a photochemical immobilization technique in order to bind antibodies vertically on the gold surface, which could improve the detection limit of colorimetric biosensors [107]. Finally, Fe 3 O 4 @Au NPs were used for Pb 2+ detection obtaining a detection limit of 15 pM.…”

Section: Gold (Au)mentioning

confidence: 99%

Synthesis and Modification of Magnetic Nanoparticles for Biosensing and Bioassay Applications: A Review

Carinelli,

Luis-Sunga,

González-Mora

et al. 2023

Chemosensors

View full text Add to dashboard Cite

Biosensors are analytical devices that use biological interactions to detect and quantify single molecules, clinical biomarkers, contaminants, allergens, and microorganisms. By coupling bioreceptors with transducers, such as nucleic acids or proteins, biosensors convert biological interactions into electrical signals. Electrochemical and optical transductions are the most widely used methods due to their high detection capability and compatibility with miniaturization. Biosensors are valuable in analytical chemistry, especially for health diagnostics, as they offer simplicity and sensitivity. Despite their usefulness, challenges persist in immobilizing biorecognition elements on the transducer surface, leading to issues such as loss of sensitivity and selectivity. To address these problems, the introduction of nanomaterials, in particular magnetic nanoparticles (MNPs) and magnetic beads, has been implemented. MNPs combine their magnetic properties with other interesting characteristics, such as their small size, high surface-to-volume ratio, easy handling, and excellent biocompatibility, resulting in improved specificity and sensitivity and reduced matrix effects. They can be tailored to specific applications and have been extensively used in various fields, including biosensing and clinical diagnosis. In addition, MNPs simplify sample preparation by isolating the target analytes via magnetic separation, thus reducing the analysis time and interference phenomena and improving the analytical performance of detection. The synthesis and modification of MNPs play a crucial role in adjusting their properties for different applications. This review presents an overview of the synthesis and surface modifications of magnetic nanoparticles and their contributions to the development of biosensors and bioassays for their applications across different areas. The future challenges of MNP synthesis and integration in assays are focused on their stability, multiplex detection, simplification and portability of test platforms, and in vivo applications, among other areas of development.

show abstract

Section: Gold (Au)mentioning

confidence: 99%

Synthesis and Modification of Magnetic Nanoparticles for Biosensing and Bioassay Applications: A Review

Carinelli,

Luis-Sunga,

González-Mora

et al. 2023

Chemosensors

View full text Add to dashboard Cite

show abstract

Synthesis and Modification of Magnetic Nanoparticles for Biosensing and Bioassay Applications: A Review

Carinelli¹,

Luis-Sunga²,

González–Mora³

et al. 2023

Preprint

View full text Add to dashboard Cite

Biosensors are analytical devices that utilize biological interactions to detect and quantify clinical biomarkers, contaminants, allergens, and microorganisms. They combine different disciplines including analytical chemistry, molecular biology, and electrical engineering. Biosensors operate by coupling a bioreceptor, such as nucleic acid or proteins, with a transducer that converts the biological interaction into an electrical signal. Electrochemical and optical transduction are commonly used approaches due to their high detection capability and compatibility with miniaturization. Biosensors provide both high specificity and sensitivity and can be integrated into low-cost microfluidic platforms for rapid and point-of-care applications. These attributes make these devices valuable tools in analytical chemistry, particularly for early diagnostic applications. However, conventional biosensors face challenges related to the immobilization of biorecognition elements on the transducer surface, leading to issues like lost of sensitivity and selectivity. To address these problems, the introduction of nanomaterials, particularly magnetic nanoparticles (MNPs) and magnetic beads (MBs), has been implemented. MNPs combine their magnetic properties with other interesting characteristics such as small size, high surface-to-volume ratio, and excellent biocompatibility. They can be tailored for specific applications and have been extensively used in various fields, including biosensing and clinical diagnosis. Furthermore, MNPs simplify sample preparation by isolating target analytes through magnetic separation, thus improving sensitivity, and reducing analysis time and interference phenomena. The synthesis and modification of MNPs play a crucial role in adjusting their properties for different applications. This review presents an overview of the synthesis and surface modifications of magnetic nanoparticles, their role in the development of biosensors and bioassays, and their applications across various scientific areas.

show abstract

Gold Coated Nanoparticles Functionalized by Photochemical Immobilization Technique for Immunosensing

Cited by 2 publications

References 19 publications

Synthesis and Modification of Magnetic Nanoparticles for Biosensing and Bioassay Applications: A Review

Synthesis and Modification of Magnetic Nanoparticles for Biosensing and Bioassay Applications: A Review

Synthesis and Modification of Magnetic Nanoparticles for Biosensing and Bioassay Applications: A Review

Contact Info

Product

Resources

About