Gold nanoisland structures integrated in a lab-on-a-chip for plasmonic detection of bovine growth hormone

Ozhikandathil, Jayan; Bădilescu, Simona; Packirisamy, Muthukumaran

doi:10.1117/1.jbo.17.7.077001

Cited by 30 publications

(33 citation statements)

References 39 publications

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“…The process of formation of widely separated nano-islands from the nanocluster is explained in [21]. In the present work, the dependence of the morphology and optical property of the nanostructure on annealing temperature is explained.…”

Section: Manufacturing Of Nano-islands On a Substratementioning

confidence: 86%

“…The diameter of the gold nano-hemisphere was set at 70 nm. The size of the nano-island was chosen at 70 nm as the proposed process of morphology tuning results in a nano-island morphology having an average size of 70 nm as explained in reference [21] and also briefly in Experimental Section (Section 4). The simulation shows an optical absorbance spectrum having a LSPR peak at 543 nm.…”

Section: Fdtd Modelingmentioning

confidence: 99%

“…Hence the process of formation nanostructures on the substrate with suitable LSPR band is an important task in the development of nano-biosensor. The gold nano-islands obtained by the proposed low-cost convective assembly and annealing have been already demonstrated for the detection of growth hormone in a microfluidic chip in our previous work [21]. …”

Section: Introductionmentioning

confidence: 99%

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Simulation and Implementation of a Morphology-Tuned Gold Nano-Islands Integrated Plasmonic Sensor

Ozhikandathil

Packirisamy

2014

Sensors

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This work presents simulation, analysis and implementation of morphology tuning of gold nano-island structures deposited by a novel convective assembly technique. The gold nano-islands were simulated using 3D Finite-Difference Time-Domain (FDTD) techniques to investigate the effect of morphological changes and adsorption of protein layers on the localized surface plasmon resonance (LSPR) properties. Gold nano-island structures were deposited on glass substrates by a novel and low-cost convective assembly process. The structure formed by an uncontrolled deposition method resulted in a nano-cluster morphology, which was annealed at various temperatures to tune the optical absorbance properties by transforming the nano-clusters to a nano-island morphology by modifying the structural shape and interparticle separation distances. The dependence of the size and the interparticle separation distance of the nano-islands on the LSPR properties were analyzed in the simulation. The effect of adsorption of protein layer on the nano-island structures was simulated and a relation between the thickness and the refractive index of the protein layer on the LSPR peak was presented. Further, the sensitivity of the gold nano-island integrated sensor against refractive index was computed and compared with the experimental results.

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Section: Manufacturing Of Nano-islands On a Substratementioning

confidence: 86%

Section: Fdtd Modelingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Simulation and Implementation of a Morphology-Tuned Gold Nano-Islands Integrated Plasmonic Sensor

Ozhikandathil

Packirisamy

2014

Sensors

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“…A shift towards the longer wavelength of the local LSPR band is noticed upon the binding of bio-molecules onto the metal nanoparticles immobilized on a substrate. While a great number of publications exist on the LSPR phenomenon and its applications, there are only a few trying to integrate LSPR biosensors and microfluidics [3][4]. Recently microfluidics-based designs have been developed and implemented by several researchers in order to isolate and quantify biomolecules which are bio markers for the early diagnosis of cancer.…”

Section: Extended Abstractmentioning

confidence: 99%

“…As mentioned earlier, the sensing mechanism makes use of the change in the position of the Au LSPR band due to the binding of the chemical entities and biomolecules to the immobilized gold nano-island, resulting in a shift of the band towards longer wavelengths. For the capture and detection of exosomes, in the present study, multilayers of gold (Au) nanoparticles were deposited by convective assembly from the gold colloidal suspension [4]. The glass substrates were further annealed at 560°C in order to modify the morphology from the multilayers of gold nanoparticles aggregates to nano-islands.…”

Section: Extended Abstractmentioning

confidence: 99%

Gold Nano-Island Platform for the Microfluidic Plasmonic Detection of Exosomes

Bathini¹,

Raju²,

Bădilescu³

et al. 2018

International Conference of Theoretical and Applied Nanoscience and Nanotechnology

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Extended AbstractNoble metals (gold and silver) possess interesting plasmonic properties, allowing the detection at the nano-scale is by observing their LSPR band shift, due to binding events and change of the refractive index [1][2]. A shift towards the longer wavelength of the local LSPR band is noticed upon the binding of bio-molecules onto the metal nanoparticles immobilized on a substrate. While a great number of publications exist on the LSPR phenomenon and its applications, there are only a few trying to integrate LSPR biosensors and microfluidics [3][4]. Recently microfluidics-based designs have been developed and implemented by several researchers in order to isolate and quantify biomolecules which are bio markers for the early diagnosis of cancer. Exosomes are the carriers of genetical and molecular information from parent to distal cells [5]. At present, the commonly used protocols for the isolation and detections of exosomes are the high-speed centrifugation and ultracentrifugation which are time consuming, with relatively low yield. Therefore, a versatile and efficient technique is required to for the isolation and detections of exosomes for further diagnosis at clinical level.The objective of the present work is the plasmonic detection of exosomes based on the sensitivity of the Au LSPR band to binding events. As mentioned earlier, the sensing mechanism makes use of the change in the position of the Au LSPR band due to the binding of the chemical entities and biomolecules to the immobilized gold nano-island, resulting in a shift of the band towards longer wavelengths. For the capture and detection of exosomes, in the present study, multilayers of gold (Au) nanoparticles were deposited by convective assembly from the gold colloidal suspension [4]. The glass substrates were further annealed at 560°C in order to modify the morphology from the multilayers of gold nanoparticles aggregates to nano-islands. In order to capture the exosomes, the affinity based synthetic polypeptide called Venceremin, Vn96 is used [6]. This molecule is specifically designed and validated to capture exosomes, having a strong affinity towards the canonical heat shock proteins (HSP), present on the surface of exosomes.The detection method is initially carried out at the substrate level, by performing the sensing protocol in a discontinuous manner. The concentrations of the chemical and biological entities at each stage of biosensing were optimized in order to facilitate the transfer to the microfluidics stage. The results indicated that this platform, is promising for the detection of exosomes. Further, in order to enhance the sensitivity of the detection and to accomplish the molecular profiling of the captured exosomes, microfluidic devices were designed, developed and tested.The results indicated that label-free technique, based on the sensitivity of the Au-LSPR band to the surrounding environment is promising for the detection of MCF-7 exosomes by the affinity approach, using the Vn96 polypeptide. This approach seems to be...

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