Gold nanoparticle-based lateral flow immunoassay for the rapid detection of flumetralin in orange

Abstract

Residues of flumetralin, a dinitroaniline herbicide, in foods pose a health risk to humans. In this study, we produced a sensitive monoclonal antibody (mAb) against flumetralin and developed a gold...

Fluorescent strip sensor for rapid and ultrasensitive determination of fluoroquinolones in fish and milk

“…The mouse with the highest OD 450 nm values and inhibition ratio was selected to be sacrificed for the preparation of spleen cells, and fusion of splenocytes and Sp2/ 0 myeloma cells was performed using PEG 4000 in a sterile environment. [35][36][37] The fused cells were distributed into 96-well plates and cultured in HAT medium for the first five days to inhibit the growth of unfused myeloma cells or hybrid cells formed by conspecific cell fusion, and then transferred into the normal HT medium for subsequent culture. After 7 days of cell fusion, ic-ELISA was used to test the cell supernatant in 96-well plates.…”