Gold nanoparticle-based lateral flow immunoassay for the rapid detection of flutriafol residues in food

Abstract

As a commonly used crop fungicide with proven adverse effects against various organisms, the excessive use of flutriafol (FTF) has resulted in high residues found in crops. In this study,...

Development of a gold nanoparticle-based lateral flow immunoassay for the fast detection of diafenthiuron in cabbage and apples

“…GNPs are negatively charged in a weakly basic environment and can be rmly bound to the positively charged groups on the mAbs through electrostatic interactions, forming GNP-mAbs that do not affect the biological properties of the antibody. [30][31][32] Specically, the pH of a 10 mL GNP solution was adjusted to 8.2 with 0.2 M K 2 CO 3 , and then 0.2 mg mL −1 of antibody solution (in 0.1 M BB buffer) was added dropwise and mixed. Aer standing for 30 min at room temperature, 500 mL of 10% BSA solution was added and incubated for 2 h at 37 °C to block sites on the surface of the GNPs that did not bind to the mAb, and nally the mixture was centrifuged at 7000×g for 45 min at 4 °C.…”

Monoclonal antibody production and development of immunochromatographic strip assays for screening of the herbicide bispyribac-sodium in rice

“…The target BIS was conjugated to carrier proteins using the 1ethyl-3-(3-dimethylaminopropyl) carbodiimide method. 13,14 Fig. 1 shows the synthesis of the immune antigen (BIS-BSA) and the coating antigen (BIS-OVA).…”

“…17,18 Aer four rounds of subcloning using the limited gradient dilution method, the hybridoma cell lines against BIS were screened. [19][20][21] The selected hybridoma cell lines were injected into a mouse aer expanding in culture. Ascites was collected from the abdominal cavity of the mouse and puried by Protein-G affinity chromatography following the manufacturer's instructions to obtain antibodies.…”

“…Rice samples were obtained from local markets and extracted as previously reported. 21,31 The rice was crushed in a pulverizer, and 10 g of rice our sample was added into 15 mL ultrapure water and oscillated for 3 h at RT. Then, the soaked sample was extracted with 60 mL of ethanol/water (4/6 v/v) by shaking for 30 min.…”

Preparation of monoclonal antibodies against norovirus and establishment of a rapid immunochromatographic technique

“…After three rounds of subcloning of hybridoma cells, single cell clusters were cultured and injected into the abdominal cavity of mice to induce formation of ascites. 28 Finally, the prepared ascites was purified using protein G columns. The obtained mAbs were characterized by evaluating protein purity, affinity and subtype.…”