Gold Nanoparticle Based Microbial Detection and Identification

A Simple Gold Nanoprobe Assay for the Identification of Staphylococcus Aureus, Listeria Monocytogenes and Salmonella Enteritidis in Food Specimens

“…To date, the existing technology for the detection of food-borne pathogens only meets the criteria of speed, sensitivity, and specificity when it uses large, laboratory-based, sophisticated and costly equipment (Hoorfar, 2011) Therefore, the development of a simplified, cost-effective and accurate procedure for detection of trace amounts of bacterial pathogens, which does not require complex instruments, seems to be imperious in order to overcome the time delay and allow rapid and sensitive detection (McGrath, Elliot, & Fodey, 2012) in infrastructure deprived environments. Nanotechnology, and more specifically gold nanoparticle chemistry, provides opportunities for the rapid and simple diagnosis of many infectious diseases, being able to detect trace amounts of bacteria, due to their uniqueoptical properties (Agasti et al, 2010;Syed & Bokhari, 2011). In the present study we developed and evaluated a gold nanoprobe assay, which does not require the amplification of the target DNA, but relies on the hybridization of the pathogen's DNA with gold nanoprobes with subsequent colorimetric differentiation.…”

A Novel Biological Synthesis of Gold Nanoparticle by <i>Enterobacteriaceae<i/> Family

“…Metallic nanoparticles can be synthesized through different methods such as spark discharging, electrochemical reduction, solution irradiation and cryochemical synthesis [7]. Although chemical reduction is the most applied method for the preparation of metallic nanoparticles, biological or green methods of nanoparticles synthesis would be preferable due to its eco-friendly properties.…”

Nanoparticles based DNA conjugates for detection of pathogenic microorganisms

“…Conjugation of NPs with antibodies in the form of immunechromatographic strip (ICS) has already been commercialized. Antibodies offer an advantage in detection as they can be targeted to not only the target microorganism but also to the toxins secreted and the antibodies generated by immune response [6,7]. Antibody coupled NPs have been designed for the detection of S. aureus in clinical samples, E. coli O157:H7 in food samples, multiplex detection of E. coli, S. typhimurium and S. aureus, detection and photo thermal lysis of S. typhimurium and detection of various others [48][49][50][51][52][53].…”

“…One of the major challenges being faced by the medical fraternity is the early and accurate diagnosis of such diseases. Conventional and reliable techniques have been optimized and practiced since decades but suffer with the disadvantages of being time consuming, expensive and may be unsuitable at times [6]. Though considerable improvement in the diagnostic techniques has been seen in the recent past, identification of slow growing and fastidious pathogens has not been achieved with much success [7].…”