Gold-nanoparticle-based multiplex immuno-strip biosensor for simultaneous determination of 83 antibiotics

Lei, Xianlu; Xu, Xinxin; Liu, Liqiang; Xu, Liguang; Wang, Li; Kuang, Hua; Xu, Chuanlai

doi:10.1007/s12274-022-4762-z

Cited by 25 publications

(8 citation statements)

References 45 publications

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“…The positive wells were screened by indirect ELISA and were subcloned using the limiting dilution method. 28 Purified A29, A27, and 162 proteins were diluted to 100 ng mL −1 as coating antigens, and positive hybridoma cells with a strong specific response to A29 protein only were selected by indirect ELISA screening. When the positive rate reached 100%, the culture was expanded.…”

Section: Preparation Of the Monoclonal Antibodiesmentioning

confidence: 99%

Gold-based paper for antigen detection of monkeypox virus

Lei

et al. 2023

Analyst

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Section: Preparation Of the Monoclonal Antibodiesmentioning

confidence: 99%

Gold-based paper for antigen detection of monkeypox virus

Lei

et al. 2023

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“…The results obtained from this study showed ISB enables a simultaneous analysis of multiple classes of antibiotic residues in aquaculture fish using a single multiplex test system. It is a simple, cost-effective, reliable and high-performing method with significant potential for on-site analysis of antibiotic residues in aquaculture fish, providing rapid and relatively comprehensive results [ 108 ].…”

Section: Analysis Of Drug Residues In Foodmentioning

confidence: 99%

Recent Advances in the Determination of Veterinary Drug Residues in Food

Pratiwi,

Ramadhanti,

Amatulloh

et al. 2023

Foods

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The presence of drug residues in food products has become a growing concern because of the adverse health risks and regulatory implications. Drug residues in food refer to the presence of pharmaceutical compounds or their metabolites in products such as meat, fish, eggs, poultry and ready-to-eat foods, which are intended for human consumption. These residues can come from the use of drugs in the field of veterinary medicine, such as antibiotics, antiparasitic agents, growth promoters and other veterinary drugs given to livestock and aquaculture with the aim of providing them as prophylaxis, therapy and for promoting growth. Various analytical techniques are used for this purpose to control the maximum residue limit. Compliance with the maximum residue limit is very important for food manufacturers according to the Food and Drug Administration (FDA) or European Union (EU) regulations. Effective monitoring and control of drug residues in food requires continuous advances in analytical techniques. Few studies have been reviewed on sample extraction and preparation techniques as well as challenges and future directions for the determination of veterinary drug residues in food. This current review focuses on the overview of regulations, classifications and types of food, as well as the latest analytical methods that have been used in recent years (2020–2023) for the determination of drug residues in food so that appropriate methods and accurate results can be used. The results show that chromatography is still a widely used technique for the determination of drug residue in food. Other approaches have been developed including immunoassay, biosensors, electrophoresis and molecular-based methods. This review provides a new development method that has been used to control veterinary drug residue limit in food.

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“…Nanoparticles (having size in range of 100 nm) have received significant attention due to the unique physical and chemical properties that arise primarily from their dimensions. These properties make them suitable in a broad array of applications ( Hashem and Salem, 2022 ; Hashem et al, 2023 ) including gas sensors ( Shin et al, 2023 ), lithium-ion batteries ( Fang et al, 2023 ), dye-sensitized solar cells ( Jin et al, 2023 ), adsorption ( Debnath and Das, 2023 ), heterogeneous photodegradation ( Mota et al, 2023 ), biosensing ( Lei et al, 2023 ), and antibacterial substrates ( lashin et al, 2023 ; Al-Askar et al, 2023 ). Additionally, NPs have shown a vast range of biological applications, including antibacterial, anticancer, and antioxidant properties ( Singh et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of gold and silver nanoparticles using crude extract of Aconitum violaceum and evaluation of their antibacterial, antioxidant and photocatalytic activities

Ahmad,

et al. 2024

Front. Bioeng. Biotechnol.

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Green synthesis of metal nanoparticles (NPs) has received extensive attention over other conventional approaches due to their non-toxic nature and more biocompatibility. Herein we report gold and silver NPs (AuNPs@AV and AgNPs@AV) prepared by employing a green approach using crude extract of Aconitum violaceum Jacquem. ex Stapf. The synthesized NPs were characterized using Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray (EDX), X-ray Diffraction (XRD), UV/Visible spectroscopy, Fourier Transform Infrared (FTIR), X-ray Photoelectron Spectroscopy (XPS), and Zeta Potential. Morphological analysis showed spherical and triangular shapes of the NPs with average size of <100 nm. The AuNPs@AV and AgNPs@AV exhibited effective antibacterial activities, with minimum inhibitory concentrations (MICs) of 95 and 70 μg/mL against Lactobacillus acidophilus (L. acidophilus) and 90 and 65 μg/mL against Escherichia coli (E. coli), respectively. Strong antioxidant effect of AuNPs@AV and AgNPs@AV were reported against DPPH radical and PTIO within range of IC50 values; 161–80 μg/ml as compared to the standard (23–11 μg/mL) respectively. Moreover, the AuNPs@AV and AgNPs@AV showed efficient photocatalytic activity and degraded 89.88% and 93.7% methylene blue (MB) dye under UV light, respectively.

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Gold-nanoparticle-based multiplex immuno-strip biosensor for simultaneous determination of 83 antibiotics

Cited by 25 publications

References 45 publications

Gold-based paper for antigen detection of monkeypox virus

Gold-based paper for antigen detection of monkeypox virus

Recent Advances in the Determination of Veterinary Drug Residues in Food

Green synthesis of gold and silver nanoparticles using crude extract of Aconitum violaceum and evaluation of their antibacterial, antioxidant and photocatalytic activities

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