Gold nanoparticle based Tuberculosis immunochromatographic assay: The quantitative ESE Quanti analysis of the intensity of test and control lines

Mdluli, Phumlane Selby; Tetyana, Phumlani; Sosibo, Ndabenhle; Walt, Hendriëtte van der; Mlambo, Mbuso; Skepu, Amanda; Tshikhudo, Robert

doi:10.1016/j.bios.2013.10.019

Cited by 28 publications

(9 citation statements)

References 22 publications

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“…This detection conjugate is further labeled by anti-protein A at control lines and fusion antigen MTB 6-14-38 kDa at test line as the capture proteins. In TB positive sample the fusion antigen labelled with 40 nm gold nanoparticles in strip rapidly capture monoclonal antibody and produce positive result (Maduli et al 2014). The effect of the analyte concentration on the MTB lateral flow assay is obtained from an ESE Quanti reader.…”

Section: Fluorescent Nanodiagnosis a Based On Lateral Flow Immunochromatography Assaymentioning

confidence: 99%

Prospects and Application of Nanotechnology for Diagnosis of Tuberculosis in Livestock: A Review

Bharti¹,

Verma²,

Dubey³

et al. 2021

IJAR

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Tuberculosis (TB) is an infectious and contagious disease caused by various strains of Mycobacterium and the foremost leading cause of death of animal and human population worldwide. Currently available conventional and modern diagnostic techniques are useful but they are neither rapid nor cost effective for early and accurate diagnosis, represents a cornerstone to eradicate TB worldwide by 2030. To overcome this awful situation an innovative strategy i.e. nanotechnology is helpful for early diagnosis of tuberculosis in short time with increased sensitivity and specificity through nano sized biomolecular interaction viz. enhanced visualization of fluorescent signals, visual colorimetric signals of amplified DNA product, nanofluidic technology, nuclear magnetic resonance technology (NMR) and prototype miniaturized device etc. Nanotechnology has introduced new paradigms for molecular diagnostics. However, some constraints of nanodiagnostic tool still there that make obstacle in its worldwide diagnostic use for TB.

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Section: Fluorescent Nanodiagnosis a Based On Lateral Flow Immunochromatography Assaymentioning

confidence: 99%

Prospects and Application of Nanotechnology for Diagnosis of Tuberculosis in Livestock: A Review

Bharti¹,

Verma²,

Dubey³

et al. 2021

IJAR

View full text Add to dashboard Cite

show abstract

“…However, for increased sensitivity, gold enhancement , silver enhancement (Anfossi et al, 2013b), and the use of gold-functionalized secondary antibodies (Laderman et al, 2008) are some of the strategies used. Mdluli et al (2014) described a lateral flowbased method for the detection M. tuberculosis 38 kDa monoclonal antibody using an AuNP conjugate functionalized with protein A as the reporter and an antigen as the immobilizer. Mdluli et al (2014) described a lateral flowbased method for the detection M. tuberculosis 38 kDa monoclonal antibody using an AuNP conjugate functionalized with protein A as the reporter and an antigen as the immobilizer.…”

Section: Lateral Flow Assaysmentioning

confidence: 99%

Gold and Silver Nanoparticles for Diagnostics of Infection

Pedrosa

Baptista

2015

Nanotechnology in Diagnosis, Treatment and Prophylaxis of Infectious Diseases

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“…Different labels are used in a LFB, and the most widely used label is gold nanoparticles as the color can be seen by the naked eye (Posthuma-Trumpie et al, 2009;Pyo and Yoo, 2012;Ren et al, 2014). LFBs have been established for the detection of various targets, such as cells, heavy metals, microRNA, viruses and bacteria (Liu et al, 2009;Chai et al, 2010;Wu et al, 2013;Mdluli et al, 2014;Gao et al, 2016;Huang et al, 2016;Jiao et al, 2019;Li et al, 2020). LFBs have developed rapidly in recent years due to their low-cost, portability and specificity.…”

Section: Introductionmentioning

confidence: 99%

Development of a Novel Lateral Flow Biosensor Combined With Aptamer-Based Isolation: Application for Rapid Detection of Grouper Nervous Necrosis Virus

et al. 2020

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Nervous necrosis virus (NNV) has infected more than 50 fish species worldwide, and has caused serious economic losses in the aquaculture industries. However, there is no effective antiviral therapy. The development of a rapid and accurate point-of-care diagnostic method for the prevention and control of NNV infection is urgently required. Commonly used methods for NNV detection include the cell culture-based assay, antibody-based assay and polymerase chain reaction (PCR)-based assay. However, these methods have disadvantages as they are time-consuming and complex. In the present study, we developed a simple and sensitive aptamer-based lateral flow biosensor (LFB) method for the rapid detection of red-spotted grouper nervous necrosis virus (RGNNV). An aptamer is a single-stranded nucleotide, which can specifically bind to the target and has many advantages. Based on a previously selected aptamer, which specifically bound to the coat protein of RGNNV (RGNNV-CP), two modified aptamers were used in this study. One aptamer was used for magnetic bead enrichment and the other was used for isothermal strand displacement amplification (SDA). After amplification, the product was further tested by the LFB, and the detection results were observed by the naked eye within 5 min with high specificity and sensitivity. The LFB method could detect RGNNV-CP protein as low as 5 ng/mL or 5 × 10 3 RGNNVinfected GB (grouper brain) cells. Overall, it is the first application of a LFB combined with aptamer in the rapid diagnosis of virus from aquatic animals, which provides a new option for virus detection in aquaculture.

show abstract

Gold nanoparticle based Tuberculosis immunochromatographic assay: The quantitative ESE Quanti analysis of the intensity of test and control lines

Cited by 28 publications

References 22 publications

Prospects and Application of Nanotechnology for Diagnosis of Tuberculosis in Livestock: A Review

Prospects and Application of Nanotechnology for Diagnosis of Tuberculosis in Livestock: A Review

Gold and Silver Nanoparticles for Diagnostics of Infection

Development of a Novel Lateral Flow Biosensor Combined With Aptamer-Based Isolation: Application for Rapid Detection of Grouper Nervous Necrosis Virus

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