Gold Nanoparticle Contrast in a Phantom and Juvenile Swine

Boote, Evan J.; Fent, Genevieve M.; Kattumuri, Vijaya; Casteel, Stan W.; Katti, Kavita; Chanda, Nripen; Kannan, Raghuraman; Churchill, Robert

doi:10.1016/j.acra.2010.01.006

Cited by 83 publications

(27 citation statements)

References 21 publications

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“…AuNp were first described by Hainfield et al (2006) for small animal imaging using X-rays and have since become available commercially for preclinical use as AuroVist (Nanoprobes Inc., NY). The work of Boote et al (2010) demonstrated the application of AuNp as a contrast agent with a clinical CT scanner. The potential of using Au for molecular imaging with CT also has been recognized in cancer (Popovtzer et al , 2008; Reuveni et al , 2011; Ghann et al , 2012; Chanda et al , 2011).…”

Section: Introductionmentioning

confidence: 99%

In vivocharacterization of tumor vasculature using iodine and gold nanoparticles and dual energy micro-CT

et al. 2013

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Tumor blood volume and vascular permeability are well established indicators of tumor angiogenesis and important predictors in cancer diagnosis, planning, and treatment. In this work, we establish a novel preclinical imaging protocol which allows quantitative measurement of both metrics simultaneously. First, gold nanoparticles are injected and allowed to extravasate into the tumor, and then liposomal iodine nanoparticles are injected. Combining a previously optimized dual energy micro-CT scan using high-flux polychromatic x-ray sources (energies: 40 kVp, 80 kVp) with a novel post-reconstruction spectral filtration scheme, we are able to decompose the results into 3D iodine and gold maps, allowing simultaneous measurement of extravasated gold and intravascular iodine concentrations. Using a digital resolution phantom, the mean limits of detectability (mean CNR = 5) for each element are determined to be 2.3 mg/mL (18 mM) for iodine and 1.0 mg/mL (5.1 mM) for gold, well within the observed in vivo concentrations of each element (I: 0-24 mg/mL, Au: 0-9 mg/mL) and a factor of 10 improvement over the limits without post-reconstruction spectral filtration. Using a calibration phantom, these limits are validated and an optimal sensitivity matrix for performing decomposition using our micro-CT system is derived. Finally, using a primary mouse model of soft-tissue sarcoma, we demonstrate the in vivo application of the protocol to measure fractional blood volume and vascular permeability over the course of five days of active tumor growth.

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Section: Introductionmentioning

confidence: 99%

In vivocharacterization of tumor vasculature using iodine and gold nanoparticles and dual energy micro-CT

et al. 2013

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“…DLS studies of the AuNPs revealed an increase in the hydrodynamic diameter as the serum proteins form the monolayer. The measurement of charge on NPs, zeta-potential (ζ), provides crucial information on the stability of the NP dispersion [22][23][24][25]. The magnitude of the measured ζ is an indication of the repulsive forces that are present and can be used to predict the long-term stability of the nanoparticulate dispersion.…”

Section: Np Size Characterization and Size Distributionmentioning

confidence: 99%

Novel nanochemistry toward generation and stabilization of gold nanoparticles in human serum albumin matrix

Kannan

Nune

Chanda

et al. 2011

Pure and Applied Chemistry

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The interactions of gold nanoparticles (AuNPs) with human serum albumin (HSA) greatly influence their in vivo characteristics. It is important to develop conjugates that can serve as ideal structural models to understand the interaction of AuNPs with HSA. We report the synthesis and stabilization of AuNPs in HSA matrix with no additional ligands on the surface of the NPs. The hydrodynamic size of the AuNP-HSA conjugate is 22 nm, and transmission electron microscopy (TEM) measurement shows the core size as 8-13 nm. We have performed strip assay to establish that the biological activity of HSA is retained even after conjugation. Our cellular toxicity evaluation studies show that AuNP-HSA conjugates are nontoxic and biocompatible.

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“…Gold nanostructures are often used to overcome the limitations found with standard contrast agents [359][360][361][362]. In addition to their biocompatibility, Au nanoparticles have longer in vivo circulation times during the imaging process and in some cases faster elimination time post-procedure [356,[363][364][365].…”

Section: Imagingmentioning

confidence: 99%

Controllable Cobalt Oxide/Au Hierarchically Nanostructured Electrode for Nonenzymatic Glucose Sensing

2016

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By electrodeposition and galvanic replacement reaction, we developed a facile, time-saving, cost-effective, and environmentally friendly, two-step synthesis route to obtain a controllable cobalt oxide/Au hierarchically nanostructured electrode for glucose sensing. The nanomaterials were characterized by transmission electron microscopy, scanning electron microscopy, Raman spectroscopy, energy-dispersive spectrometry, and X-ray photoelectron spectroscopy, meanwhile, the sensing performance was investigated by cyclic voltammetry and amperometric response. The results revealed that this novel electrode exhibited excellent electrocatalytic performance toward glucose oxidation, with a wide double-linear range from 0.2 μM to 20 mM and a low detection limit of 0.1 μM based on a signal-to-noise ratio of 3, which was mainly attributed to the ability of loading a small amount of Au with good electron conductivity on the surface of cobalt oxide nanosheets with large active surface area and synergistic electrocatalytic activity of Au and cobalt oxide toward glucose electrooxidation. This facile, sensitive, and selective glucose sensor is also proven to be suitable for the detection of glucose in human serum.

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Gold Nanoparticle Contrast in a Phantom and Juvenile Swine

Cited by 83 publications

References 21 publications

In vivocharacterization of tumor vasculature using iodine and gold nanoparticles and dual energy micro-CT

In vivocharacterization of tumor vasculature using iodine and gold nanoparticles and dual energy micro-CT

Novel nanochemistry toward generation and stabilization of gold nanoparticles in human serum albumin matrix

Controllable Cobalt Oxide/Au Hierarchically Nanostructured Electrode for Nonenzymatic Glucose Sensing

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