Gold nanoparticles amplified microcantilever biosensor for detecting protein biomarkers with high sensitivity

Zhao, Junyuan; Wang, Lihao; Fu, Dengyuan; Zhao, Dongyan; Wang, Yübo; Yuan, Quan; Zhu, Yong; Yang, Ji; Yang, Fuhua

doi:10.1016/j.sna.2021.112563

Cited by 31 publications

(6 citation statements)

References 36 publications

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“…The sensor demonstrated a LOD of 0.6 ng/mL and a linear range of detection spanning from 1 to 900 ng/mL. Zhao et al developed a sensor for detecting AFP utilizing a microcantilever biosensor with AuNPs amplification [81]. This innovative approach enhances sensitivity to AFP.…”

Section: Mass-based Cantilever Sensor-based Detection Of Alpha-fetopr...mentioning

confidence: 99%

Biosensing of Alpha-Fetoprotein: A Key Direction toward the Early Detection and Management of Hepatocellular Carcinoma

Ramachandran,

Abul Rub,

Hajja

et al. 2024

Biosensors

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Hepatocellular carcinoma (HCC) is currently one of the most prevalent cancers worldwide. Associated risk factors include, but are not limited to, cirrhosis and underlying liver diseases, including chronic hepatitis B or C infections, excessive alcohol consumption, nonalcoholic fatty liver disease (NAFLD), and exposure to chemical carcinogens. It is crucial to detect this disease early on before it metastasizes to adjoining parts of the body, worsening the prognosis. Serum biomarkers have proven to be a more accurate diagnostic tool compared to imaging. Among various markers such as nucleic acids, circulating genetic material, proteins, enzymes, and other metabolites, alpha-fetoprotein (AFP) is a protein marker primarily used to diagnose HCC. However, current methods need a large sample and carry a high cost, among other challenges, which can be improved using biosensing technology. Early and accurate detection of AFP can prevent severe progression of the disease and ensure better management of HCC patients. This review sheds light on HCC development in the human body. Afterward, we outline various types of biosensors (optical, electrochemical, and mass-based), as well as the most relevant studies of biosensing modalities for non-invasive monitoring of AFP. The review also explains these sensing platforms, detection substrates, surface modification agents, and fluorescent probes used to develop such biosensors. Finally, the challenges and future trends in routine clinical analysis are discussed to motivate further developments.

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Section: Mass-based Cantilever Sensor-based Detection Of Alpha-fetopr...mentioning

confidence: 99%

Biosensing of Alpha-Fetoprotein: A Key Direction toward the Early Detection and Management of Hepatocellular Carcinoma

Ramachandran,

Abul Rub,

Hajja

et al. 2024

Biosensors

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“…Starting in 2014, Wang et al, reported a series of works about microcantilever integration, including surface antibody modification [ 179 , 180 ], and the design and fabrication of a microcantilever with cavity, piezoelectric drive, and frequency-tracking circuit [ 181 , 182 , 183 ]. The latest research of this team, in 2021 [ 184 ], had a microcantilever prepared on an SOI substrate by standard CMOS processes such as RIE and PECVD. After modification by the antigen–antibody system, the microcantilever was integrated into a microfluidic chip, as shown in Figure 14 E. The integrated detection system can detect alpha-fetoprotein under the amplification of nanoparticles.…”

Section: Application Of Genetic-probe-modified Cantilevermentioning

confidence: 99%

“… ( A ) Integrated testing equipment of Lechuga et al [ 67 ]; ( B ) integrated testing equipment of Ricciardi et al [ 175 ]; ( C ) integrated testing equipment of Huang et al [ 176 ]; ( D ) integrated testing equipment of Khemthongcharoen et al [ 142 ]; ( E ) integrated testing equipment of Wang et al [ 184 ]. …”

Section: Figurementioning

confidence: 99%

A Genosensor Based on the Modification of a Microcantilever: A Review

et al. 2023

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When the free end of a microcantilever is modified by a genetic probe, this sensor can be used for a wider range of applications, such as for chemical analysis, biological testing, pharmaceutical screening, and environmental monitoring. In this paper, to clarify the preparation and detection process of a microcantilever sensor with genetic probe modification, the core procedures, such as probe immobilization, complementary hybridization, and signal extraction and processing, are combined and compared. Then, to reveal the microcantilever’s detection mechanism and analysis, the influencing factors of testing results, the theoretical research, including the deflection principle, the establishment and verification of a detection model, as well as environmental influencing factors are summarized. Next, to demonstrate the application results of the genetic-probe-modified sensors, based on the classification of detection targets, the application status of other substances except nucleic acid, virus, bacteria and cells is not introduced. Finally, by enumerating the application results of a genetic-probe-modified microcantilever combined with a microfluidic chip, the future development direction of this technology is surveyed. It is hoped that this review will contribute to the future design of a genetic-probe-modified microcantilever, with further exploration of the sensitive mechanism, optimization of the design and processing methods, expansion of the application fields, and promotion of practical application.

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“…Mass-based sensors are of particular interest for the detection of diseases such as cancer where early diagnosis can lead to better patient outcomes. Several research groups have explored the use of silicon, ceramics, carbon nanotubes, and/or gold nanoparticles in mass-based sensors for the detection of cancer biomarkers. − Su et al developed a novel piezoelectric biosensor using a lead titanate zirconate ceramic resonator and successfully detected alpha fetoprotein (AFP) and prostate-specific antigen (PSA) with a sensitivity of 0.25 ng/mL within 30 min by using only 1 μL of sample . Ding et al used an assembly of gold nanoparticles on nano-hydroxyapatite for the interface of a QCM immunosensor, on which antibodies were immobilized for AFP detection in the concentration range of 15–600 ng/mL .…”

Section: Introductionmentioning

confidence: 99%

Antibody Immobilization on Sulfated Cellulose Nanocrystals

et al. 2023

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Exploiting cellulose nanocrystals’ high aspect ratio and tailorable surface for immunological biosensors has been hindered by the relatively limited research on using commonly available sulfated cellulose nanocrystals (CNCs) for antibody immobilization and by the low hydrolytic stability of dried assemblies produced from sulfated CNCs. Herein, we report a reaction scheme that enables both hydrolytic stability and antibody immobilization through 3-aminopropyl-triethoxysilane and glutaric anhydride chemistry. Immobilization was demonstrated using three model antibodies used in the detection of the cancer biomarkers: alpha-fetoprotein, prostate-specific antigen, and carcinoembryonic antigen. Thermogravimetric analysis coupled with Fourier-transform infrared spectroscopy provided evidence of CNC modification. Quartz crystal microbalance with dissipation monitoring was used to monitor binding during each step of the immobilization scheme as well as binding of the corresponding antigens. The general reaction scheme was tested using both aqueous CNC dispersions and CNC films. Film modification is slightly simpler as it avoids centrifugation and washing steps. However, modifying the dispersed CNCs provides access to their entire surface area and results in a greater capacity for antigen binding.

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Gold nanoparticles amplified microcantilever biosensor for detecting protein biomarkers with high sensitivity

Cited by 31 publications

References 36 publications

Biosensing of Alpha-Fetoprotein: A Key Direction toward the Early Detection and Management of Hepatocellular Carcinoma

Biosensing of Alpha-Fetoprotein: A Key Direction toward the Early Detection and Management of Hepatocellular Carcinoma

A Genosensor Based on the Modification of a Microcantilever: A Review

Antibody Immobilization on Sulfated Cellulose Nanocrystals

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