Gold nanoparticles as a factor of influence on doxorubicin–bovine serum albumin complex

Goncharenko, N. A.; Pavlenko, O. L.; Dmytrenko, О. P.; Kulish, M. P.; Lopatynskyi, A. M.; Chegel, V. I.

doi:10.1007/s13204-018-0748-2

Cited by 14 publications

(3 citation statements)

References 42 publications

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“…As the concentration of protein molecules in solution increases, the concentration of nanoparticles that did not precipitate over a long period of aging increases as a result of the formation of stabilizing BSA-AuNP conjugates. At the concentration of BSA Here, the concentration dependence of optical density is completely opposite to the one for fresh DOX-BSA solutions without AuNPs (Goncharenko et al 2018), where AuNP conjugates with DOX and BSA were absent. This implies a decrease of DOX-BSA complexation in the presence of citrate-stabilized nanoparticles due to conjugation processes of AuNPs with DOX and BSA.…”

Section: Resultsmentioning

confidence: 84%

“…In addition, the plasmon oscillations are strongly dependent not only on the geometry and size of the nanoparticles, but also on the physical and chemical properties of the surrounding environment (Lopatynskyi et al 2011;Chegel et al 2012). Moreover, the addition of AuNPs to the drugs solutions is accompanied not only by the spectral changes due to the self-association of these nanoparticles, but also by electron-conformational transformations of molecules, including DOX (Goncharenko et al 2018). While the optical absorption spectrum of DOX covering the nanoparticles exhibits minor changes, there is a significant quenching of its fluorescence band and transformation of the IR absorption stretching vibrational band of the amino group (Dhar et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Understanding prodrugs: complexation in aqueous solutions of doxorubicin, bovine serum albumin and gold nanoparticles

et al. 2019

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Aqueous solutions of doxorubicin (DOX), doxorubicin with bovine serum albumin (DOX-BSA), and DOX-BSA in solutions containing gold nanoparticles (AuNPs) with different concentrations of each system component are studied for the first time. The study of the concentration and time-resolved dependences of optical density spectra indicates that the interaction of protonated molecules of the antibiotic with the citrate-covered surface of the nanoparticles of high concentration promotes the aggregation and precipitation of AuNPs. On the other hand, protein macromolecules effectively form conjugates with nanoparticles, preventing their aggregation. Competition of these processes significantly complicates the behavior of optical density spectra at the absorption band of doxorubicin. The simultaneous processes of AuNP aggregation and BSA-AuNP association allow reasonable result of calculation of equilibrium-binding constants for the DOX-BSA complexation process to be obtained only at low nanoparticle concentrations (up to 1.3 × 10 −10 M). It was found that these constants decrease to K = 1.18 × 10 6 M −1 compared with K = 4.74 × 10 6 M −1 for the DOX-BSA complex, indicating lower stability of these complexes after citrate-stabilized nanoparticles addition. The number of binding sites in DOX-BSA complexes, as shown by the analysis of the isomolar series curves, stays the same for the indicated AuNPs concentrations and is equal to n = 2. The obtained results could be useful to clarify the appropriate concentration of such type of AuNPs upon the development of oncology prodrugs.

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Section: Resultsmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Understanding prodrugs: complexation in aqueous solutions of doxorubicin, bovine serum albumin and gold nanoparticles

et al. 2019

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“…At the same time, the induced PL quenching is observed in a wide range of concentrations from 1 × 10 −9 M to 1 × 10 −4 M. Moreover, there is a significant difference in the quenching values for concentrations C-107 1 × 10 −9 M, 1 × 10 −8 M and higher concentration values of 1 × 10 −5 M, 1 × 10 −4 M. The [17][18][19][20][21]. It can be assumed that the known PL quenching mechanisms described by the Stern-Volmer equation or its modifications cannot be used for this system.…”

Section: Pl Quenching In Water Solutions Of Atp and Calix[4]arenementioning

confidence: 72%

Peculiarities of the Fluorescence Quenching in the ATP – Calix[4]arene C-107 Aqueous Solutions

Starzhynska,

Dmytrenko,

Kulish

et al. 2024

Ukr. J. Phys.

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The nature of fluorescence (FL) quenching for the aqueous solutions of adenosine triphosphate (ATP) with calix[4]arenes C-107 in the presence of silver nitrate AgNO3 is studied. It is shown that, for the water solutions of ATP and calix[4]arenes C-107 at a constant concentration of ATP molecules with an increase in the content of C-107, a complex nature of the PL quenching is observed, while maintaining the position of the PL band near 395 nm (λex = 285 nm). Its complexity is based, on the one hand, in the wide range of concentrations of C-107, at which it occurs, and, on the other hand, there are gaps in the quenching values for individual concentrations of calix[4]arene, near which it changes slightly. The indicated nature of the PL quenching significantly depends on the wavelength of excitation and the temperature. Similar quenching behavior is preserved, when AgNO3s alts are added to the ATP–C-107 mixtures, (CATP = CC-107 = 1 × 10−4M) in the concentration range from 1 × 10−4M to 1 × 10−3M. The computer modeling shows that the system ATP–C-107 can form energetically stable complexes, when ATP is located on the top of the calix[4]arene and along the wall of it due to π-π-stacking interaction, and the complexes are characterized by a shrinking of the energy bands.

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Mechanisms of Heteroassociation of Ceftriaxone and Doxorubicin Drugs with Bovine Serum Albumin

Dmytrenko

Kulish

Pavlenko

et al. 2021

Springer Proceedings in Physics

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Gold nanoparticles as a factor of influence on doxorubicin–bovine serum albumin complex

Cited by 14 publications

References 42 publications

Understanding prodrugs: complexation in aqueous solutions of doxorubicin, bovine serum albumin and gold nanoparticles

Understanding prodrugs: complexation in aqueous solutions of doxorubicin, bovine serum albumin and gold nanoparticles

Peculiarities of the Fluorescence Quenching in the ATP – Calix[4]arene C-107 Aqueous Solutions

Mechanisms of Heteroassociation of Ceftriaxone and Doxorubicin Drugs with Bovine Serum Albumin

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