Gold nanoparticles chemisorbed by a terphenyldithiol self-assembled monolayer for fabrication of a protein biosensor

Abstract

In this study, improved detection of bovine serum albumin (BSA) was achieved by use of a fabricated surface plasmon resonance (SPR) biosensor. Terphenyldithiol (TPDT) was self-assembled on a gold substrate, then gold nanoparticles (Au-NPs) were chemisorbed on to the TPDT monolayer by strong bonding with the terminal thiol groups of the TPDT. The new sensor obtained was tested for immobilization of protein. The SPR results revealed much better detection of BSA by Au-NPs chemisorbed on the TPDT self-assembled monolayer (SAM) than by the bare SAM on the gold substrate.