Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/&amp;beta;-catenin signaling pathway

Choi, Seon Young; Song, Min Seok; Ryu, Pan Dong; Lam, Anh Thu N.; Joo, Sang‐Woo; Lee, So Yeong

doi:10.2147/ijn.s78775

Cited by 63 publications

(23 citation statements)

References 58 publications

(65 reference statements)

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“…In spite of this, most studies focus on the pathophysiology and not on the physiology of AuNPs. Multiple in-vitro studies have shown that AuNPs at a certain particle size and concentration promote cell proliferation [ 4 , 10 ]. Our previous experiments confirmed that 60 nm AuNPs at a concentration of 56 μM significantly promote the proliferation of human periodontal ligament cells and hPDLSCs [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

“…Our previous experiments confirmed that 60 nm AuNPs at a concentration of 56 μM significantly promoted the proliferation of hPDLSCs, while exerting no apparent side effects on the differentiation potential of cells. However, another study reported that AuNPs mainly promote the differentiation of human adipose-derived mesenchymal stem cells (MSCs) in osteogenesis via various signaling pathways [ 10 ]. Moreover, AuNPs of different sizes can affect the physical processes of cells via diverse signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

“…This process is established on canonical Wnt signaling, which increases nuclear and cytoplasmic β-catenin and initiates transcriptional activators such as MYC and CCND1 [ 12 ]. Recently, Wnt signaling has been implicated in the control of MSC differentiation, including osteogenic, chondrogenic, adipogenic, and myogenic differentiation [ 10 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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The role of the Wnt/β-catenin signaling pathway in the proliferation of gold nanoparticle-treated human periodontal ligament stem cells

Zhang

et al. 2018

Stem Cell Res Ther

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BackgroundSeveral studies have confirmed that gold nanoparticles (AuNPs) of specific concentration and size exert a boosting effect on cell proliferation; however, the mechanism through which this effect occurs remains unknown. This study explores the canonical Wnt signaling pathway in AuNP promotion of human periodontal ligament stem cell (hPDLSC) proliferation.MethodsMTS was employed to evaluate hPDLSC proliferation. The interference of LRP5 and β-catenin was steered by shRNA plasmids and siRNA, respectively, at which point the expression of MYC, CCND1, AXIN2, and POU5F1 had been estimated via real-time PCR. The expressions of LRP5 and β-catenin were detected via western blot assay.ResultsThe proliferation of hPDLSCs treated with 60 nm AuNPs at 56 μM was clearly elevated. In contrast, β-catenin siRNA significantly decreased cell viability. The LRP5 shRNA plasmid did not consistently impact cells. The expressions of these four genes downstream of the Wnt/β-catenin signaling pathway were not significantly overexpressed in response to the interference of shRNA plasmid/siRNA with the treatment of AuNPs.ConclusionsThese results suggest that the Wnt/β-catenin signaling pathway plays a significant role in the process of AuNP promotion of hPDLSC proliferation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The role of the Wnt/β-catenin signaling pathway in the proliferation of gold nanoparticle-treated human periodontal ligament stem cells

Zhang

et al. 2018

Stem Cell Res Ther

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“…Previously, AuNPs, functionalized with polymers such as chitosan-conjugated AuNPs, were developed to achieve advanced differentiation of human mesenchymal Stem Cells (hMSCs) [ 65 ]. Chitosan, a type of aminated polysaccharide that has been utilized in bone tissue engineering, shows similarity to glycosaminoglycan, which plays an important role in extracellular matrix (ECM) interaction during cell adhesion.…”

Section: Main Textmentioning

confidence: 99%

Two-dimensional material-based bionano platforms to control mesenchymal stem cell differentiation

et al. 2018

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BackgroundIn the past decade, stem cells, with their ability to differentiate into various types of cells, have been proven to be resourceful in regenerative medicine and tissue engineering. Despite the ability to repair damaged parts of organs and tissues, the use of stem cells still entails several limitations, such as low differentiation efficiency and difficulties in guiding differentiation. To address these limitations, nanotechnology approaches have been recently implemented in stem cell research. It has been discovered that stem cells, in combination with carbon-based functional materials, show enhanced regenerative performances in varying biophysical conditions. In particular, several studies have reported solutions to the conventional quandaries in biomedical engineering, using synergetic effects of nanohybrid materials, as well as further development of technologies to recover from diverse health conditions such as bone fracture and strokes.Main textIn this review, we discuss several prior studies regarding the application of various nanomaterials in controlling the behavior of stem cells. We focus on the potential of different types of nanomaterials, such as two-dimensional materials, gold nanoparticles, and three-dimensional nanohybrid composites, to control the differentiation of human mesenchymal stem cells (hMSCs). These materials have been found to affect stem cell functions via the adsorption of growth/differentiation factors on the surfaces of nanomaterials and the activation of signaling pathways that are mostly related to cell adhesion and differentiation (e.g., FAK, Smad, Erk, and Wnt).ConclusionControlling stem cell differentiation using biophysical factors, especially the use of nanohybrid materials to functionalize underlying substrates wherein the cells attach and grow, is a promising strategy to achieve cells of interest in a highly efficient manner. We hope that this review will facilitate the use of other types of newly discovered and/or synthesized nanomaterials (e.g., metal transition dichalcogenides, non-toxic quantum dots, and metal oxide frameworks) for stem cell-based regenerative therapies.

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“…Gold nanoparticles were investigated to promote the osteogenic differentiation of adipose derived stem cells (ADSCs) [ 60 ]. Chitosan-conjugated AuNPs increased the calcium content and expression of osteogenic genes at non-toxic levels [ 61 ]. The size of the nanoparticles can influence the effect the differentiation of stem cells.…”

Section: Nanoparticlesmentioning

confidence: 99%

An update on the Application of Nanotechnology in Bone Tissue Engineering

Griffin¹,

Kalaskar²,

Seifalian³

et al. 2016

TOORTHJ

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Background: Natural bone is a complex and hierarchical structure. Bone possesses an extracellular matrix that has a precise nano-sized environment to encourage osteoblasts to lay down bone by directing them through physical and chemical cues. For bone tissue regeneration, it is crucial for the scaffolds to mimic the native bone structure. Nanomaterials, with features on the nanoscale have shown the ability to provide the appropriate matrix environment to guide cell adhesion, migration and differentiation.Methods:This review summarises the new developments in bone tissue engineering using nanobiomaterials. The design and selection of fabrication methods and biomaterial types for bone tissue engineering will be reviewed. The interactions of cells with different nanostructured scaffolds will be discussed including nanocomposites, nanofibres and nanoparticles.Results: Several composite nanomaterials have been able to mimic the architecture of natural bone. Bioceramics biomaterials have shown to be very useful biomaterials for bone tissue engineering as they have osteoconductive and osteoinductive properties. Nanofibrous scaffolds have the ability to provide the appropriate matrix environment as they can mimic the extracellular matrix structure of bone. Nanoparticles have been used to deliver bioactive molecules and label and track stem cells.Conclusion:Future studies to improve the application of nanomaterials for bone tissue engineering are needed.

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Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway

Cited by 63 publications

References 58 publications

The role of the Wnt/β-catenin signaling pathway in the proliferation of gold nanoparticle-treated human periodontal ligament stem cells

The role of the Wnt/β-catenin signaling pathway in the proliferation of gold nanoparticle-treated human periodontal ligament stem cells

Two-dimensional material-based bionano platforms to control mesenchymal stem cell differentiation

An update on the Application of Nanotechnology in Bone Tissue Engineering

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