Gold Nanopellets a Unique Platform for Electrochemical Ultra-Low Detection of Ochratoxin A

Basu, Tinku

doi:10.24966/fsn-1076/100032

Cited by 2 publications

(2 citation statements)

References 29 publications

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“…The indium tin oxide glass (ITO) substrate of 1.5 cm × 1 cm size was cleaned thoroughly before use and air dried. Then, the ITO substrate was dipped in 10% HCl solution for about 10 min followed by annealing at the temperature ranges of 450–500 °C for 7 h and suddenly cooled to normal room temperature [ 15 , 25 ].…”

Section: Methodsmentioning

confidence: 99%

“…These present a real bottleneck in terms of providing a rapid diagnosis. Currently, electrochemical biosensors are viewed as a potential alternative to the analytical gold standard method for detection of cTnI and other biomarkers with respect to their rapid sensing and a user friendly approach due to its simplicity, enhanced sensitivity, selectivity, low detection limit, less sample volume, cost-effectiveness, and less time consumption qualities [ 14 , 15 ]. The presence of cTnI in serum is sensed through the specific binding of anti-cTnI with cTnI antigen and not with globular protein.…”

Section: Introductionmentioning

confidence: 99%

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Template Free Anisotropically Grown Gold Nanocluster Based Electrochemical Immunosensor for Ultralow Detection of Cardiac Troponin I

et al. 2022

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Anisotropic gold nanostructures have fascinated with their exceptional electronic properties, henceforth exploited for the fabrication of electrochemical sensors. However, their synthesis approaches are tedious and often require a growth template. Modern lifestyle has caused an upsurge in the risk of heart attack and requires urgent medical attention. Cardiac troponin I can serve as a biomarker in identification of suspected myocardial infection (heart attack). Hence the present work demonstrates the fabrication of a sensing platform developed by assimilating anisotropic gold nanoclusters (AuNCs) with anti cTnI antibody (acTnI) for the detection of cardiac troponin I (cTnI). The uniqueness and ease of synthesis by a template-free approach provides an extra edge for the fabrication of AuNC coated electrodes. The template-free growth of anisotropic AuNCs onto the indium tin oxide (ITO) glass substrates offers high sensitivity (2.2 × 10−4 A ng−1 mL cm−2) to the developed sensor. The immunosensor was validated by spiking different concentrations of cTnI in artificial serum with negligible interference under optimized conditions. The sensor shows a wide range of detection from 0.06–100 ng/mL with an ultralow detection limit. Thus, it suggests that the template-free immunosensor can potentially be used to screen the traces of cTnI present in blood serum samples, and the AuNCs based platform holds great promise as a transduction matrix, hence it can be exploited for broader sensing applications.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%