Gold-silver core-shell nanoparticle–based impedimetric immunosensor for detection of iron homeostasis biomarker hepcidin

Rana, Shilpa; Bharti, Anu; Singh, Suman; Bhatnagar, Archana; Prabhakar, Nirmal

doi:10.1007/s00604-020-04599-8

Cited by 11 publications

(9 citation statements)

References 43 publications

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“…Ten microliters of aqueous cysteamine solution (10 mM) was drop casted onto the Au/MoS 2 /FTO electrode for 4 h at RT. Cysteamine acts as a bi-functional agent, -SH group of cysteamine forms SAM on Au/MoS 2 /FTO electrode, and the free -NH 2 group interacts with -COOH [28]. After 4 h, the electrodes were washed consecutively with distilled water, and 5 μL of Ab-25(OH)D 3 solution (15 μg mL −1 ) was casted onto the Cys/Au/MoS 2 /FTO electrode and incubated at 4°C for 12 h in a humid chamber.…”

Section: Immobilization Of Bio-recognition Elementmentioning

confidence: 99%

“…A selfassembled monolayer of cysteamine was also introduced onto the Au/MoS 2 /FTOs to allow the chemical binding of Ab-25(OH)D 3 via amide bond formation between amine group of cysteamine and carboxylic group at Fc region of antibody. This not only allows the chemical binding of antibody onto the electrode surface but also promotes its controlled and directional binding rendering the antigen binding site of antibody free for efficient interaction with analyte [28].…”

Section: Choice Of Materialsmentioning

confidence: 99%

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Voltammetric detection of vitamin D employing Au-MoS2 hybrid as immunosensing platform

et al. 2021

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A voltammetric immunosensor based on molybdenum sulphide (MoS 2 ) and gold nanoparticles (Au NPs) for the determination of 25-hydroxy vitamin D 3 (25(OH)D 3 ) is reported. Anti-vit D (Ab-25(OH)D 3 ) was immobilized onto the cysteamine-modified MoS 2 and Au NPs which were deposited onto a fluoride tin oxide (FTO) electrode (Ab/Cys/Au/MoS 2 /FTO). The MoS 2 sheets were prepared by hydrothermal method followed by an in situ growth of Au film onto the MoS 2 /FTO surface. Self-assembled monolayer (SAM) of cysteamine was synthesized onto the Au/MoS 2 /FTO which acts as a linker to covalently bind Ab-25(OH)D 3 . The Ab-25(OH)D 3 -immobilized Cys/Au/MoS 2 /FTO was used to detect 25(OH)D 3 using differential pulse voltammetry. The electrochemical system provided an anodic peak current at a potential of +0.21 V vs. Ag/AgCl (satd. KCl) of ferricyanide/ferrocyanide redox couple. The detection principle relies on the inhibition of electron transfer at the electrode surface owing to the hindrance caused by the formation of immune complex between Ab-25(OH)D 3 and 25(OH)D 3 . The immunosensor shows linear response from 1 pg mL −1 to 100 ng mL −1 25(OH)D 3 and a sensitivity of 189 μA [log (pg mL −1 )] −1 cm −2 along with a low limit of detection (LOD) of 0.38 pg mL −1 . The immunosensor is highly selective towards 25(OH)D 3 and presented a long shelf life of 28 days. Also, the immunosensor exhibits satisfactory performance towards spiked human serum samples with recovery between 95.1 and 102% (RSD 1.15-3.22%).

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Section: Immobilization Of Bio-recognition Elementmentioning

confidence: 99%

Section: Choice Of Materialsmentioning

confidence: 99%

Voltammetric detection of vitamin D employing Au-MoS2 hybrid as immunosensing platform

et al. 2021

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“…Compared with other precious metal nanoparticles, Au nanoparticles have special advantages because of its high catalysis, excellent optics, and electrical conductivity. 22,24 Particularly, Au nanoparticles are favored for its outstanding detection performance. [25][26][27][28][29] However, there are also some limitations in the application of Au nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Au nanoparticle-loaded eggshell for electrochemical detection of nitrite

et al. 2021

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“…Different sp 3 or sp 2 hybrid of carbons and oxygen at the edges with different functional groups makes GO has many active sites and fast electronic transmission [15], and biocompatibility. Similar advantages in gold nanoparticles (AuNPs) can further enhance the electron transfer ability [16,17,18]. However, due to the relatively small size, AuNPs are easy to aggregrate and migrate.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Probe of the Reduced Graphene Oxide Modified by Bare Gold Nanoparticles Functionalized Zr(IV)‐based Metal‐organic Framework for Detecting Nitrite

Chen

et al. 2021

Electroanalysis

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Widely presented nitrite in drinking water, food and even physiological system endangers human health. Here,bare gold nanoparticles functionalized Zr-based metal-organic framework modified reduced graphene oxide (GNPs/UiO-66-NH 2 /rGO) nanocomposites were prepared by hydrothermal method. This experiment studies the morphology, composition, structure and electrochemical behavior of the sensor. The experimental results show that the sensor has a peak potential of 0.9 V, the concentration range of NO 2 À is 5.0 μM to 768 μM, the linear regression equation of the calibration curve is I pa = 0.3646 + 0.00642 C (R 2 = 0.9998), and the LOD is as low as 3.7 μM (S/N = 3). Therefore, an electrochemical sensor platform for trace detection of NO 2 À was successfully constructed.

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Gold-silver core-shell nanoparticle–based impedimetric immunosensor for detection of iron homeostasis biomarker hepcidin

Cited by 11 publications

References 43 publications

Voltammetric detection of vitamin D employing Au-MoS2 hybrid as immunosensing platform

Voltammetric detection of vitamin D employing Au-MoS2 hybrid as immunosensing platform

Au nanoparticle-loaded eggshell for electrochemical detection of nitrite

Electrochemical Probe of the Reduced Graphene Oxide Modified by Bare Gold Nanoparticles Functionalized Zr(IV)‐based Metal‐organic Framework for Detecting Nitrite

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