Golgi Apparatus: Influence on Cell Surfaces

Whaley, W. Gordon; Dauwalder, Marianne; Kephart, Joyce E.

doi:10.1126/science.175.4022.596

Cited by 115 publications

(15 citation statements)

References 69 publications

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“…They stressed that the enzyme was detected on the inside face of GF~ and GFz vesicles but on the outside of GF3, and suggested that GF3 may represent secretory vesicles which fuse with the surface membrane on discharging their content. One can also interpret GF3 as surface membrane, if the surface membrane is considered to have a patchwork composition (43) and include Golgi-like parts (43).…”

Section: Discussionmentioning

confidence: 99%

Synthesis of plasmalemmal glycoproteins in intestinal epithelial cells. Separation of Golgi membranes from villus and crypt cell surface membranes; glycosyltransferase activity of surface membrane

Weiser¹,

Neumeier²,

Quaroni³

et al. 1978

The Journal of Cell Biology

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The relationship between Golgi and cell surface membranes of intestinal cells was studied. These membranes were isolated from intestinal crypt cells and villus cells. The villus cell membranes consisted of microvillus membrane, a Golgi-rich fraction, and two membrane fractions interpreted as representing lateral-basal membranes. The villus cell microvillus membrane was purified by previously published techniques while the other membranes were obtained from isolated cells by differential centrifugation and density gradient velocity sedimentation. The two membrane fractions obtained from villus cells and considered to be lateral-basal membranes were enriched for Na+,K+-ATPase activity, but one also showed enrichment in glycosyltransferase activity. The Golgi membrane fraction was enriched for glycosyltransferase activity and had low to absent Na+,K +-ATPase activity. Adenylate cyclase activity was present in all membrane fractions except the microvillus membrane but co-purified with Golgi rather than lateralbasal membranes. Electron microscopy showed that the Golgi fraction consisted of variably sized vesicles and cisternalike structures. The two lateral-basal membrane fractions showed only vesicles of smaller, more uniform size. After 125I labeling of isolated intact cells, radioactivity was found associated with the lateralbasal and microvillus membrane fractions and not with the Golgi fraction. Antibody prepared against lateral-basal membrane fractions reacted with the surface membrane of isolated villus cells. The membrane fractions from isolated crypt cells demonstrated that all had high glycosyltransferase activity. The data show that glycosyltransferase activity, in addition to its Golgi location, may be a significant property of the lateral-basal portion of the intestinal villus cell plasma membrane. Data obtained with crypt cells support earlier data and show that the crypt cell surface membrane possesses glycosyltransferase activity. 722J. CELL BIOLOGY 9 The Rockefeller University Press 9

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Section: Discussionmentioning

confidence: 99%

Synthesis of plasmalemmal glycoproteins in intestinal epithelial cells. Separation of Golgi membranes from villus and crypt cell surface membranes; glycosyltransferase activity of surface membrane

Weiser¹,

Neumeier²,

Quaroni³

et al. 1978

The Journal of Cell Biology

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“…Depuis peu on pense que les acides sialiques de surface sont impliqués dans l'organisation structurale et les fonctions de l'enveloppe cellulaire : reconnaissance cellule-cellule et cellule-substrat, adhésion spécifique (Weiss 1969, Winzler 1970, Whaley 1972) ; toute modification de leur taux peut influer sur les caractéristiques de l'hématie. Il était intéressant de rechercher si les modifications membranaires érythrocytaires déjà citées, étaient associées à des altérations des glucides de surface.…”

Section: Introductionunclassified

Plasmodium chabaudi : Modification des acides sialiques de surface des hématies au cours de l’infestation

Fabia

Gattegno

et al. 1979

Ann. Parasitol. Hum. Comp.

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Résumé.Les acides sialiques de surface des hématies de souris infestées par le parasite Plasmodium chabaudi sont augmentés comparativement à ceux d'hématies de souris non infestées. Cette augmentation coïncide avec celle des réticulocytes circulants. Une augmen tation similaire des acides sialiques de surface est montrée par l'étude des réticulocytes induits par phénylhydrazine. De la même façon, les oses neutres et le fucose sont trouvés en quantité plus importante dans les glycopeptides trypsiques de surface desréticulocytes que dans les glycopeptides trypsiques de surface des hématies. Cette augmentation se retrouve dans les hématies de souris infestées par Plasmodium chabaudi, parallèlement à la valeur de la réticulocytémie. Ceci suggère la contribution significative des réticulocytes aux variations biochimiques observées des hématies infestées au cours de la malaria.

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“…Packaging and transport of secretory proteins (4) and biosynthesis of glycoproteins (5), important both in secretion and as cell surface determinants of the plasma membrane (6), have been attributed to the Golgi apparatus as well as a role in lipid and lipoprotein secretion (7) . Further understanding of the structural and functional organization of the Golgi apparatus requires quantitative morphometric analysis with biochemical studies, so that variation in structure or arrangement of the membranes may be related to changes in their activity .…”

Section: Introductionmentioning

confidence: 99%