Gomisin M2 Ameliorates Atopic Dermatitis-like Skin Lesions via Inhibition of STAT1 and NF-κB Activation in 2,4-Dinitrochlorobenzene/Dermatophagoides farinae Extract-Induced BALB/c Mice

Kang, Jinjoo; Lee, So‐Young; Kim, Nam-Kyung; Dhakal, Hima; Kwon, Taeg‐Kyu; Kim, Eun-Nam; Jeong, Gil‐Saeng; Kim, Sang-Hyun

doi:10.3390/molecules26154409

Cited by 6 publications

(4 citation statements)

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“…By suppressing the activation of keratinocytes, the major epidermal cells actively involved in AD HDMs are the unsurpassed cause of allergic diseases such as AD, asthma, and rhinitis throughout the world, and Dermatophagoides farinae used in the experiment is the major species of HDM [8]. Based on the previous studies, DNCB was used as a hapten for pre-sensitization, and DFE was used as an allergen to induce AD-like skin inflammation in this study [44,45]. Repeated applications of DNCB/DFE showed common phenotypes of AD, including erythema, scaling, hyperplasia, thickened skin as well as infiltration of immune cells.…”

Section: Discussionmentioning

confidence: 99%

Lupeol alleviates atopic dermatitis-like skin inflammation in 2,4-dinitrochlorobenzene/Dermatophagoides farinae extract-induced mice

Bae

Jeong

Choi

et al. 2023

BMC Pharmacol Toxicol

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Background Atopic dermatitis (AD) is a chronic inflammatory skin disease that affects from children to adults widely, presenting symptoms such as pruritus, erythema, scaling, and dryness. Lupeol, a pentacyclic triterpenoid, has anti-inflammatory and antimicrobial activities. Based on these properties, the therapeutic effects of lupeol on skin disorders have been actively studied. In the present study, we aimed to determine the effectiveness of lupeol on AD. Methods We utilized tumor necrosis factor (TNF)-α/interferon (IFN)-γ-stimulated keratinocytes and 2, 4-dinitrochlorobenzene/Dermatophagoides farinae extract (DFE)-induced AD mice to confirm the action. Results Lupeol inhibited TNF-α/IFN-γ-stimulated keratinocytes activation by reducing the expressions of pro-inflammatory cytokines and chemokines which are mediated by the activation of signaling molecules such as signal transducer and activator of transcription 1, mitogen-activated protein kinases (p38 and ERK), and nuclear factor-κB. Oral administration of lupeol suppressed epidermal and dermal thickening and immune cell infiltration in ear tissue. Immunoglobulin (Ig) E (total and DFE-specific) and IgG2a levels in serum were also reduced by lupeol. The gene expression and protein secretion of T helper (Th) 2 cytokines, Th1 cytokines, and pro-inflammatory cytokine in ear tissue were decreased by lupeol. Conclusions These results suggest that lupeol has inhibitory effects on AD-related responses. Therefore, lupeol could be a promising therapeutic agent for AD.

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Section: Discussionmentioning

confidence: 99%

Lupeol alleviates atopic dermatitis-like skin inflammation in 2,4-dinitrochlorobenzene/Dermatophagoides farinae extract-induced mice

Bae

Jeong

Choi

et al. 2023

BMC Pharmacol Toxicol

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“…In addition, α-cubebenoate and gomisin M2 isolated from S. chinensis prevented eosinophil and mast cell infiltration in AD mice and prevented antigen-induced degranulation in RBL-2H3 cells [ 8 , 9 ]. These results suggest that the effect of suppressing immune cell infiltration of S. chinensis can be explained by the above components.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that S. chinensis contains ingredients with antioxidant effects, so it has a skin-whitening effect and suppresses skin irritation [ 2 , 3 ]. In addition, S. chinensis has been reported to have anti-oxidative [ 2 ], anti-aging [ 4 ], anti-allergic [ 5 ], and anti-inflammatory [ 6 ] effects, and the two primary components of S. chinensis , α-cubebenoate and gomisin M2, have been found to have anti-inflammatory effects in mice with experimental AD [ 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

The Anti-Inflammatory and Skin Barrier Function Recovery Effects of Schisandra chinensis in Mice with Atopic Dermatitis

et al. 2023

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Background and Objectives: The fruit of Schisandra chinensis (Turcz.) Baill. is widely used medicinally to treat coughs, asthma, exhaustion, eczema, and pruritus in Northeast Asian countries, including Korea, China, and Japan. This study was designed to investigate the effects of S. chinensis on dermatitis in mice with calcipotriol (MC-903)-induced atopic dermatitis (AD), and its effects on skin barrier dysfunction was also investigated. Materials and Methods: The inhibitory effects of an ethanolic extract of S. chinensis (EESC) on skin lesions, water content, water-holding capacity (WHC), histopathological abnormalities, and inflammatory cytokine and chemokine levels were evaluated in mice with AD induced by MC903. Results: Topical EESC ameliorated skin lesions, reduced skin water content, and increased MC903-induced WHC. EESC also prevented MC-903-induced histopathological abnormalities such as epidermal disruption, hyperkeratosis, spongiotic changes, and immune cell infiltration in inflamed tissue. Moreover, topical EESC reduced MC-903-induced levels of pro-inflammatory cytokines and chemokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-4, IL-6, IL-8, monocyte chemotactic protein (MCP)-1, and thymic stromal lymphopoietin (TSLP). Furthermore, unlike dexamethasone, EESC did not reduce the spleen/body weight ratio. Conclusions: These results suggest that S. chinensis can be used as an alternative to external corticosteroids and that its anti-inflammatory and skin barrier dysfunction-restoring effects are related to the downregulation of pro-inflammatory cytokines and chemokines, such as TNF-α, IL-4, IL-6, IL-8, and TSLP.

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“…In addition, DNCB increases the expression of various cytokines and chemokines that cause AD [ 40 , 41 ]. The skin lesions of chronic AD have many characteristics, such as dryness, epidermis and dermis thickness, and lichenification, and AD induces various responses within the immune system, affecting the weight of immune organs such as the spleen [ 42 , 43 ]. We demonstrated that PLG reduced the severity of lesions on mice skin, epidermal and dermal thickening, and spleen weight, and demonstrated that PLG suppressed immune responses and improved histological characteristics.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effect of Isatis tinctoria L. water extract on DNCB-induced atopic dermatitis in BALB/c mice and HaCaT cells

Min

Kim

et al. 2022

Chin Med

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Background Isatis tinctoria L (PLG) is a medicinal herb from the roots of Isatis indigotica Fort (Family Cruciferae). Previous studies have shown that PLG has anti-inflammatory and therapeutic effects against conditions such as acute and chronic hepatitis, various respiratory inflammations, and cancer. The purpose of this study was to define the pharmacological effects of PLG on inflammatory reactions and skin hyperkeratosis, which are the main symptoms of atopic dermatitis (AD), in vivo and in vitro. Methods For the AD in vivo experiment, 2,4-dinitrochlorobenzene (DNCB) induction and oral administration of PLG were performed on male BALB/c mice for four weeks. For in vitro experiments, keratinocytes were activated using TNF-α/IFN-γ in cultured human keratinocyte (HaCaT) cells. PLG inhibited inflammatory chemokine production and blocked the nuclear translocation of NF-κB p65 in activated keratinocytes. Results As a result of oral administration of PLG, dermis and epidermis thickening, as well as eosinophil and mast cell infiltration, were attenuated in AD skin lesions. In addition, the levels of immunoglobulin E (IgE), pro-inflammatory cytokines, and the MAPK/NF-κB signaling pathway were decreased in serum and dorsal skin tissues. Furthermore, PLG inhibited inflammatory chemokine production and blocked the nuclear translocation of NF-κB p65 in activated keratinocytes. In addition, epigoitrin and adenosine, the standard compounds of PLG, were identified as candidate AD compounds. Conclusions These results indicate that PLG is a potent therapeutic agent for attenuating symptoms of AD.

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Gomisin M2 Ameliorates Atopic Dermatitis-like Skin Lesions via Inhibition of STAT1 and NF-κB Activation in 2,4-Dinitrochlorobenzene/Dermatophagoides farinae Extract-Induced BALB/c Mice

Cited by 6 publications

References 40 publications

Lupeol alleviates atopic dermatitis-like skin inflammation in 2,4-dinitrochlorobenzene/Dermatophagoides farinae extract-induced mice

Lupeol alleviates atopic dermatitis-like skin inflammation in 2,4-dinitrochlorobenzene/Dermatophagoides farinae extract-induced mice

The Anti-Inflammatory and Skin Barrier Function Recovery Effects of Schisandra chinensis in Mice with Atopic Dermatitis

Inhibitory effect of Isatis tinctoria L. water extract on DNCB-induced atopic dermatitis in BALB/c mice and HaCaT cells

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