Gonadal transcriptome analysis of the common carp, Cyprinus carpio: Identification of differentially expressed genes and SSRs

Anitha, Arumugam; Gupta, Yugantak Raj; Seetharam, Deepa; Ningappa, Manjappa; Rajanna, K. B.; Senthilkumaran, B.

doi:10.1016/j.ygcen.2018.12.004

Cited by 30 publications

(8 citation statements)

References 81 publications

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“…Novel_145 regulates the expression of wt1 , which has been found to have high expression in developing and adult testis of catfish and played an important role in spermatogenesis [ 63 ]. In addition, wt1 has been found to be a testis-biased gene in Silurus asotus [ 64 ], Cyprinus carpio , and tilapia [ 65 , 66 ]. In our study, novel_145 was also highly expressed in the testis of T. blochii , indicating that novel_145 may play an essential role in the development of testis.…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Sex-Biased miRNAs in the Golden Pompano (Trachinotus blochii)

Shi

Song

Zhang

et al. 2022

Animals

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The golden pompano (Trachinotus blochii) is a marine fish of considerable commercial importance in China. It shows notable sexual size dimorphism; the growth rate of females is faster than that of males. Therefore, sex-biased research is of great importance in T. blochii breeding. However, there have been few studies on sex differentiation and mechanisms underlying sex determination in T. blochii. MicroRNAs (miRNAs) play crucial roles in sex differentiation and determination in animals. However, limited miRNA data are available on fish. In this study, two small RNA libraries prepared from the gonads of T. blochii were constructed and sequenced. The RNA-seq analysis yielded 1366 known and 69 novel miRNAs with 289 significantly differentially expressed miRNAs (p < 0.05). Gene ontology (GO) analysis confirmed that the TFIIA transcription factor complex (GO: 0005672) was the most significantly enriched GO term. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the differentially expressed miRNAs and target genes were mainly related to sex determination and gonadal developmental signaling pathways, specifically the Wnt signaling pathway, MAPK signaling pathway, and steroid biosynthetic pathway. MiRNA-mRNA co-expression network analysis strongly suggested a role for sex-biased miRNAs in sex determination/differentiation and gonadal development. For example, gata4, foxo3, wt1, and sf1 genes were found to be regulated by bta-miR-2898; esr2 and foxo3 by novel_176, and ar by oar-let-7b. Quantitative real-time polymerase chain reaction analysis of selected mRNAs and miRNAs validated the integrated analysis. This study established a set of sex-biased miRNAs that are potential regulatory factors in gonadal development in T. blochii. These results provide new insight into the function of miRNAs in sex differentiation and determination in T. blochii and highlight some key miRNAs for future studies.

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Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Sex-Biased miRNAs in the Golden Pompano (Trachinotus blochii)

Shi

Song

Zhang

et al. 2022

Animals

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“…The surprising 17β-hydroxysteroid dehydrogenase activity of Japanese eel 20β-HSD type 2 with androgens was not observed for other teleost 20β-HSD type 2 enzymes with the substrate androstenedione [18,22]. Further putative 20β-HSD type 2 members in spotted gar, fugu, medaka, stickleback, and common carp were identified [20,23] but so far not characterized.…”

Section: Introductionmentioning

confidence: 94%

Functional characterization of two 20β-hydroxysteroid dehydrogenase type 2 homeologs from Xenopus laevis reveals multispecificity

Tokarz

Schmitt

Möller

et al. 2021

The Journal of Steroid Biochemistry and Molecular Biology

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“…Teleosts fishes present extraordinary plasticity and diversity of their molecular mechanisms (Bachtrog et al, 2014; Capel, 2017; Heule et al, 2014; Mank & Avise, 2009; Sun et al, 2013) differing between several taxa (Manousaki et al, 2014; Zhang, Wang, et al, 2011). However, finding sex‐related genes is necessary to elucidate the complex mechanisms of gonadal development in fish, and Next Generation Sequencing (NGS) technologies provide operational tools for high‐throughput sequencing, which will increase the efficacy and speed of new gene discovery (Anitha et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…To detect the sex of the fish, some methods for identifying sex in adult individuals have been developed using the dosage of gonadal steroids (see Chu‐Koo et al, 2009; Monteiro et al, 2010). According to Anitha et al, (2019), the wide range of sexual development systems in fishes, and the lower specialization of pirarucu explain the absence of morphologically distinct traits, as in most fish species. Sexual dimorphism will appear in higher taxons as Acantopterygii and Protacantopterygii.…”

Section: Introductionmentioning

confidence: 99%

Insight to new genes with sex‐biased to bony‐tongued fishes: Differentially expressed genes in adult individuals of Arapaima gigas revealed by RNA‐Seq

Santos

Fagundes

et al. 2021

Aquaculture Research

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The Arapaima gigas is an endemic fish to the Amazon basin and with great potential for aquaculture, although its reproduction in captivity is limited by the absence of sexual dimorphism. The demand increase for pirarucu in the Northern region of Brazil, has led to new studies on the reproduction of the species. Thus, we aimed to investigate patterns of sex-biased genes in brain and gonad tissues of A. gigas. A total of of 451,490,808 short sequencing reads were produced using the SOLiD v4 plus sequencing platform. An average of ~30% of sequencing reads were mapped to Asian arowana reference cDNAs, species that belong to the same group of bony-tongue fishes. Gene expression analysis identified 305 sex-biased genes for the brain, with 297 genes up-regulated in females and only eight genes up-regulated in males. The analysis of the gonads identified 120 sex-biased genes, with 110 genes up-regulated in females and only ten genes up-regulated in males. Gene ontology analysis found more than 50 terms enriched for the sex-biased genes in a wide range of pathways suggesting that multiple functions and processes differ between the sexes. Thus, the following areas of interest were approached: sex steroids/hormones, organism development process and macromolecule biosynthetic process, which exhibited sex-biased gene expression; these areas are known to influence sexual development in other species. Lastly, the information obtained in this study concerning the sex-biased genes in the A. gigas species is a contribution to further studies approaching the processes of gonadal development, and downstream molecular mechanisms in species.

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Gonadal transcriptome analysis of the common carp, Cyprinus carpio: Identification of differentially expressed genes and SSRs

Cited by 30 publications

References 81 publications

Identification and Characterization of Sex-Biased miRNAs in the Golden Pompano (Trachinotus blochii)

Identification and Characterization of Sex-Biased miRNAs in the Golden Pompano (Trachinotus blochii)

Functional characterization of two 20β-hydroxysteroid dehydrogenase type 2 homeologs from Xenopus laevis reveals multispecificity

Insight to new genes with sex‐biased to bony‐tongued fishes: Differentially expressed genes in adult individuals of Arapaima gigas revealed by RNA‐Seq

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