Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation

Baillon, Lucie; Oses, Jennifer; Pierron, Fabien; Colombier, Sarah Bureau du; Caron, Antoine; Normandeau, Éric; Lambert, Patrick; Couture, Patrice; Labadie, Pierre; Budzinski, Hélène; Dufour, Sylvie; Bernatchez, Louis; Baudrimont, Magalie

doi:10.1016/j.chemosphere.2015.06.007

Cited by 13 publications

(7 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For female eels, 67% of their fat store is spent on the spawning migration and oocyte maturation (Palstra & van den Thillart, ). As lipids are mobilized during spawning migration, contaminants are more likely to be released into the blood at high concentrations, thus negatively affecting gonad maturation and oocyte production, as they do in other fish species (Baillon et al., ; ICES, ; Pierron et al., ), and also impairing migration success (Geeraerts & Belpaire, ; Pierron, Baudrimont, Dufour, et al., ; Robinet & Feunteun, ). As a summary, contaminants can act as a classical stressor during the continental stage of eel, but then have the potential to dramatically impair maturation and migration success, that is the whole reproduction success.…”

Section: Component 2—increased Contamination Load: Contamination Of Ementioning

confidence: 99%

Freshwater eels: A symbol of the effects of global change

Drouineau

Durif

Castonguay³

et al. 2018

Fish and Fisheries

Self Cite

124

View full text Add to dashboard Cite

Temperate eels Anguilla anguilla (European eel), A. rostrata (American eel) and A. japonica (Japanese eel) are three catadromous species which have been declining since the 1970s/1980s despite their remarkable adaptive capacity. Because of their specific life cycles, which share distant oceanic spawning grounds and continental growth stage, eels are affected by five components of the global change: (a) climate change affecting larval survival and drift, (b) an increase in pollution leading to high levels of contamination exacerbated by their high lipid levels, (c) increasing fragmentation and habitat loss that reduce dramatically the amount of available habitats and induce increased spawner mortality, (d) the appearance of Anguillicola crassus a parasitic alien nematode that impairs spawning success, and (e) the impact of commercial and recreational fisheries for all life stages of eel. In this context, the rapid increases of pressures during the “Great Acceleration” have surpassed the adaptive capacity of eels. This illustrates that cumulative effects of global change can lead to the collapse of species, even in species that have amazingly high adaptive capacities.

show abstract

Section: Component 2—increased Contamination Load: Contamination Of Ementioning

confidence: 99%

Freshwater eels: A symbol of the effects of global change

Drouineau

Durif

Castonguay³

et al. 2018

Fish and Fisheries

Self Cite

124

View full text Add to dashboard Cite

show abstract

“…; Baillon et al . ) and A. anguilla as well as proteomes of seven model species ( Fugu rubripes, Danio rerio, Xiphophorus maculatus , Latimeria chalumnae, Oreochromis niloticus, Petromyzon marinus, Mus musculus and Homo sapien from Ensemble). We specified 200 bp for a maximum flank of evidence clusters for ab initio predictions and 10 000 bp for maximum intron length.…”

Section: Methodsmentioning

confidence: 99%

“…, ), transcriptomics response to pollutants (Baillon et al . ) and further investigation on osmotic response and fatty acid biosynthesis in Japanese eel to optimize their farming (Henkel et al . ; Tse et al .…”

Section: Introductionmentioning

confidence: 99%

Draft genome of the American Eel (Anguilla rostrata)

Pavey

Laporte

Normandeau

et al. 2016

Molecular Ecology Resources

Self Cite

View full text Add to dashboard Cite

Freshwater eels (Anguilla sp.) have large economic, cultural, ecological and aesthetic importance worldwide, but they suffered more than 90% decline in global stocks over the past few decades. Proper genetic resources, such as sequenced, assembled and annotated genomes, are essential to help plan sustainable recoveries by identifying physiological, biochemical and genetic mechanisms that caused the declines or that may lead to recoveries. Here, we present the first sequenced genome of the American eel. This genome contained 305 043 contigs (N50 = 7397) and 79 209 scaffolds (N50 = 86 641) for a total size of 1.41 Gb, which is in the middle of the range of previous estimations for this species. In addition, protein-coding regions, including introns and flanking regions, are very well represented in the genome, as 95.2% of the 458 core eukaryotic genes and 98.8% of the 248 ultra-conserved subset were represented in the assembly and a total of 26 564 genes were annotated for future functional genomics studies. We performed a candidate gene analysis to compare three genes among all three freshwater eel species and, congruent with the phylogenetic relationships, Japanese eel (A. japanica) exhibited the most divergence. Overall, the sequenced genome presented in this study is a crucial addition to the presently available genetic tools to help guide future conservation efforts of freshwater eels.

show abstract

“…However, studies carried out on the liver are invasive and involve the sacrifice of animals. Due to the critically endangered status of the European eel species, the objective of this study was to test whether the caudal fin may be used to detect in situ exposure to contaminants by means of a 1000 candidate-gene microarray (Baillon et al 2015b), developed from our previous RNA-Seq study.…”

Section: Responsible Editor: Philippe Garriguesmentioning

confidence: 99%

“…All procedures were carried out as previously described in Baillon et al (2015b). Total RNA was extracted from the caudal fin and liver of the 20 experimental fish (5 individuals per condition, 5 uncontaminated (controls), 5 contaminated by PCBs at 50 ng g −1 and 300 ng g −1 , and 5 contaminated by Cd at 4 μg L −1 ) and a total of 15 wild fish from Certes, Gironde, and Garonne sampling sites (5 specimen per sampling site).…”

Section: Rna Extraction Labeling and Cdna Hybridizationmentioning

confidence: 99%

Detecting the exposure to Cd and PCBs by means of a non-invasive transcriptomic approach in laboratory and wild contaminated European eels (Anguilla anguilla)

Baillon

Pierron

Oses

et al. 2015

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

Detecting and separating specific effects of contaminants in a multi-stress field context remain a major challenge in ecotoxicology. In this context, the aim of this study was to assess the usefulness of a non-invasive transcriptomic method, by means of a complementary DNA (cDNA) microarray comprising 1000 candidate genes, on caudal fin clips. Fin gene transcription patterns of European eels (Anguilla anguilla) exposed in the laboratory to cadmium (Cd) or a polychloro-biphenyl (PCBs) mixture but also of wild eels from three sampling sites with differing contamination levels were compared to test whether fin clips may be used to detect and discriminate the exposure to these contaminants. Also, transcriptomic profiles from the liver and caudal fin of eels experimentally exposed to Cd were compared to assess the detection sensitivity of the fin transcriptomic response. A similar number of genes were differentially transcribed in the fin and liver in response to Cd exposure, highlighting the detection sensitivity of fin clips. Moreover, distinct fin transcription profiles were observed in response to Cd or PCB exposure. Finally, the transcription profiles of eels from the most contaminated site clustered with those from laboratory-exposed fish. This study thus highlights the applicability and usefulness of performing gene transcription assays on non-invasive tissue sampling in order to detect the in situ exposure to Cd and PCBs in fish.

show abstract

Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation

Cited by 13 publications

References 37 publications

Freshwater eels: A symbol of the effects of global change

Freshwater eels: A symbol of the effects of global change

Draft genome of the American Eel (Anguilla rostrata)

Detecting the exposure to Cd and PCBs by means of a non-invasive transcriptomic approach in laboratory and wild contaminated European eels (Anguilla anguilla)

Contact Info

Product

Resources

About