Gonadectomy and blood sampling procedures in small size teleost models

Royan, Muhammad Rahmad; Kanda, Shinji; Kayo, Daichi; Song, Weiyi; Ge, Wei; Weltzien, Finn‐Arne; Fontaine, Romain

doi:10.1101/2020.08.30.271478

Cited by 3 publications

(3 citation statements)

References 58 publications

(49 reference statements)

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“…Fortunately, we have a wealth of powerful tools available to investigate the role of gonadal sex steroids. For instance, GDX, which is still used today in teleosts and adapted to new species of interest ( 308 ), is a powerful technique when combined with steroid replacement to investigate the role sex steroids play in tissue plasticity. However, GDX does not remove all sex steroids, as some can be produced by interrenal cells and adipose tissue ( 309 ), as well as in the brain and pituitary as described above.…”

Section: General Conclusion and Future Perspectivesmentioning

confidence: 99%

Direct and Indirect Effects of Sex Steroids on Gonadotrope Cell Plasticity in the Teleost Fish Pituitary

et al. 2020

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The pituitary gland controls many important physiological processes in vertebrates, including growth, homeostasis, and reproduction. As in mammals, the teleost pituitary exhibits a high degree of plasticity. This plasticity permits changes in hormone production and secretion necessary to meet the fluctuating demands over the life of an animal. Pituitary plasticity is achieved at both cellular and population levels. At the cellular level, hormone synthesis and release can be regulated via changes in cell composition to modulate both sensitivity and response to different signals. At the cell population level, the number of cells producing a given hormone can change due to proliferation, differentiation of progenitor cells, or transdifferentiation of specific cell types. Gonadotropes, which play an important role in the control of reproduction, have been intensively investigated during the last decades and found to display plasticity. To ensure appropriate endocrine function, gonadotropes rely on external and internal signals integrated at the brain level or by the gonadotropes themselves. One important group of internal signals is the sex steroids, produced mainly by the gonadal steroidogenic cells. Sex steroids have been shown to exert complex effects on the teleost pituitary, with differential effects depending on the species investigated, physiological status or sex of the animal, and dose or method of administration. This review summarizes current knowledge of the effects of sex steroids (androgens and estrogens) on gonadotrope cell plasticity in teleost anterior pituitary, discriminating direct from indirect effects.

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Section: General Conclusion and Future Perspectivesmentioning

confidence: 99%

Direct and Indirect Effects of Sex Steroids on Gonadotrope Cell Plasticity in the Teleost Fish Pituitary

et al. 2020

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“…Blood was collected from the peduncle vein using a glass capillary as described previously (Royan et al, 2020). The blood was stored at -80 °C for later measurement of steroid concentrations.…”

Section: Sample Collectionmentioning

confidence: 99%

Impact of light pollution at night on male reproductive success in Japanese medaka (Oryzias latipes)

Closs

Royan

Sayyari

et al. 2023

Preprint

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Environmental light is perceived and anticipated by organisms to synchronize their biological cycles. Therefore, exposure to artificial light at night could disrupt diurnal and seasonal rhythmicity. Reproduction is a complex physiological process involving integration of environmental signals by the brain, and release of endocrine signals by the pituitary that regulate gametogenesis and spawning. In addition, males from many species form a dominance hierarchy that, through a combination of aggressive and protective behavior, influences their reproductive success. In this study, we investigated the effect of different light regimes, including light pollution at night and continuous light, on the fitness of male fish within a dominance hierarchy using a model fish, the Japanese medaka. In normal light/dark rhythm conditions, we observed that dominant males are more aggressive, remain closer to the female, and spend ten-fold more time spawning than subordinates. By using males with different genotypes, we determined the paternity of the progeny and found that even though subordinate males spend less time with the females, they are equally successful at fertilizing eggs in normal light conditions due to an efficient sneaking behavior. However, when exposed to light at night, dominant males fertilize more eggs. We indeed found that when exposed to nocturnal light pollution, dominant males produce higher quality sperm than subordinate males. Surprisingly, we did not find differences in circulating sex steroid levels, pituitary gonadotropin levels, or gonadosomatic index between dominant and subordinate males, neither in control nor night light condition. Continuous light was found to completely inhibit establishment of male hierarchy. This study is the first to report an effect of light pollution on sperm quality with an impact on male fertilization success in any vertebrate. It has broad implications for fish ecology in urban areas with potential impacts on the genetic diversity of these fish populations.

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“…Briefly, control and sgN1b sexually mature males (n=7) were coupling with WT females (Figure 5supplement figure 1A). Males and females were separated in the evening (6-7 PM) the day before the assay, using a transparent plastic cup with small holes for water exchange and then blood samples of anesthetized male individuals were collected directly from the caudal vein using a glass capillary tube (Royan et al, 2020). All fish were recovered after blood extraction.…”

Section: Blood Levels Of 11-ketotestosterone In Sexually Mature Malesmentioning

confidence: 99%

Cystic proliferation of germline stem cells is necessary to reproductive success and normal mating behavior in medaka

Padilla

Castañeda-Cortés

Rosa

et al. 2021

eLife

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The production of an adequate number of gametes is necessary for normal reproduction, for which the regulation of proliferation from early gonadal development to adulthood is key in both sexes. Cystic proliferation of germline stem cells is an especially important step prior to the beginning of meiosis; however, the molecular regulators of this proliferation remain elusive in vertebrates. Here, we report that ndrg1b is an important regulator of cystic proliferation in medaka. We generated mutants of ndrg1b that led to a disruption of germ cells cystic proliferation. This loss of cystic proliferation was observed from embryogenic to adult stages, impacting the success of gamete production and reproductive parameters such as spawning and fertilization. Interestingly, the depletion of cystic proliferation also impacted male sexual behavior, with a decrease of mating vigor. These data illustrate why it is also necessary to consider gamete production capacity in order to analyze reproductive behavior.

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Gonadectomy and blood sampling procedures in small size teleost models

Cited by 3 publications

References 58 publications

Direct and Indirect Effects of Sex Steroids on Gonadotrope Cell Plasticity in the Teleost Fish Pituitary

Direct and Indirect Effects of Sex Steroids on Gonadotrope Cell Plasticity in the Teleost Fish Pituitary

Impact of light pollution at night on male reproductive success in Japanese medaka (Oryzias latipes)

Cystic proliferation of germline stem cells is necessary to reproductive success and normal mating behavior in medaka

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