Gonadotropin Cell Transduction Mechanisms

Mauro, Annunziata; Berardinelli, Paolo; Barboni, Barbara

doi:10.3390/ijms23116303

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…Gonadotropin-sensitive granulosa cells in growing follicles are responsible for steroidogenesis which plays a crucial role in ovarian function. 47 CYP19A1 can convert androgens to estrogens in granulosa cells. 48 However, there was no high expression of CYP19A1 but CYP11A1 was found in the yak granulosa cells (Figure 2E).…”

Section: Discussionmentioning

confidence: 99%

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A single‐cell transcriptomic atlas characterizes cell types and their molecular features in yak ovarian cortex

et al. 2022

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The ovary as one of the most dynamic organs produces steroids to orchestrate female secondary sexual characteristics, harbors ovarian reserve for oocytes, releases mature oocytes for fertilization, and maintains pregnancy. Yak (Bos grunniens) is the only bovid animal that can adapt to the harsh climatic conditions on the Qinghai‐Tibetan Plateau (altitudes of over 3000 m above sea level). However, the cellular atlas is composed of oocytes and other somatic cells, and their individual molecular characteristics remain to be elucidated in the yak ovary. Here, single‐cell RNA sequencing (scRNA‐seq) was performed to delineate the molecular signature of various cell types in the yak ovarian cortex. A cellular atlas of yak ovarian cortex was constructed successfully on the basis of the differentially expressed genes (DEGs) from the distinct cell types and their functional enrichment analysis, comprising endothelial cells, nature kill cells, stromal cells, smooth muscle cells, oocytes, macrophages, epithelial cells, and granulosa cells. Meanwhile, the signature genes were determined based on their expression specificity in each cell type. A cell‐to‐cell communication network was built in light of the differentially overexpressed ligand and receptor genes from each cell type. Further, the oocytes were subdivided into four subtypes based on their individual DEGs and the functional enrichment of the DEGs. FST and TOP2A were identified as maker genes for oocytes by immunostaining in the yak ovarian cortex. The cellular atlas reveals the biological characteristics of the ovarian cortex at the cellular molecular level and provides insights into female reproductive biology via cellular communications in the yak.

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Section: Discussionmentioning

confidence: 99%

“…A mammalian ovarian follicle consists of an innermost oocyte, surrounding granulosa cells, and thecal cells of outer layers. Gonadotropin‐sensitive granulosa cells in growing follicles are responsible for steroidogenesis which plays a crucial role in ovarian function 47 . CYP19A1 can convert androgens to estrogens in granulosa cells 48 .…”

Section: Discussionmentioning

confidence: 99%

A single‐cell transcriptomic atlas characterizes cell types and their molecular features in yak ovarian cortex

et al. 2022

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GnRH-driven FTO-mediated RNA m6A modification promotes gonadotropin synthesis and secretion

Wang,

Ma,

Zhang

et al. 2024

BMC Biol

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Background Gonadotropin precisely controls mammalian reproductive activities. Systematic analysis of the mechanisms by which epigenetic modifications regulate the synthesis and secretion of gonadotropin can be useful for more precise regulation of the animal reproductive process. Previous studies have identified many differential m6A modifications in the GnRH-treated adenohypophysis. However, the molecular mechanism by which m6A modification regulates gonadotropin synthesis and secretion remains unclear. Results Herein, it was found that GnRH can promote gonadotropin synthesis and secretion by promoting the expression of FTO. Highly expressed FTO binds to Foxp2 mRNA in the nucleus, exerting a demethylation function and reducing m6A modification. After Foxp2 mRNA exits the nucleus, the lack of m6A modification prevents YTHDF3 from binding to it, resulting in increased stability and upregulation of Foxp2 mRNA expression, which activates the cAMP/PKA signaling pathway to promote gonadotropin synthesis and secretion. Conclusions Overall, the study reveals the molecular mechanism of GnRH regulating the gonadotropin synthesis and secretion through FTO-mediated m6A modification. The results of this study allow systematic interpretation of the regulatory mechanism of gonadotropin synthesis and secretion in the pituitary at the epigenetic level and provide a theoretical basis for the application of reproductive hormones in the regulation of animal artificial reproduction.

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Single-Cell Transcriptomics Analysis Reveals a Cell Atlas and Cell Communication in Yak Ovary

Pei

Xiong

Guo

et al. 2023

IJMS

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Yaks (Bos grunniens) are the only bovine species that adapt well to the harsh high-altitude environment in the Qinghai-Tibetan plateau. However, the reproductive adaptation to the climate of the high elevation remains to be elucidated. Cell composition and molecular characteristics are the foundation of normal ovary function which determines reproductive performance. So, delineating ovarian characteristics at a cellular molecular level is conducive to elucidating the mechanism underlying the reproductive adaption of yaks. Here, the single-cell RNA-sequencing (scRNA-seq) was employed to depict an atlas containing different cell types with specific molecular signatures in the yak ovary. The cell types were identified on the basis of their specifically expressed genes and biological functions. As a result, a cellular atlas of yak ovary was established successfully containing theca cells, stromal cells, endothelial cells, smooth muscle cells, natural killer cells, macrophages, and proliferating cells. A cell-to-cell communication network between the distinct cell types was constructed. The theca cells were clustered into five subtypes based on their biological functions. Further, CYP11A1 was confirmed as a marker gene for the theca cells by immunofluorescence staining. Our work reveals an ovarian atlas at the cellular molecular level and contributes to providing insights into reproductive adaption in yaks.

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Gonadotropin Cell Transduction Mechanisms

Cited by 3 publications

References 10 publications

A single‐cell transcriptomic atlas characterizes cell types and their molecular features in yak ovarian cortex

A single‐cell transcriptomic atlas characterizes cell types and their molecular features in yak ovarian cortex

GnRH-driven FTO-mediated RNA m6A modification promotes gonadotropin synthesis and secretion

Single-Cell Transcriptomics Analysis Reveals a Cell Atlas and Cell Communication in Yak Ovary

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