Gonococcal ribosomes as skin test antigens. II. Precision Of the Method, Attempts To Identify The Ribosomal Antigen, and Correlation with The Macrophage Migration Inhibition test.

Judd, Ralph C.; Koostra, W. L.

doi:10.1136/sti.52.1.28

Cited by 3 publications

(4 citation statements)

References 14 publications

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“…Surface antigens, such as pilus, envelope, and OMC antigens, varied among strains; in addition, it has long been known that many organisms undergo reversible mutations between the piliated and nonpiliated phases (11). Furthermore, ribosomal fractions reportedly induce delayed-type hypersensitivity in guinea pigs (14,17). In this study, ribosomal fractions were further purified by affinity chromatography, designated as PRF, and used as antigens for the PHA and ELISA.…”

Section: Discussionmentioning

confidence: 98%

Passive hemagglutination test for detection of antibody to gonococcal ribosomal antigen in sera from patients with asymptomatic gonorrhea

Kita¹,

Kashiba²

1982

J Clin Microbiol

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Ribosomal fractions were obtained from a culture of type 2 Neisseria gonorrhoeae strain P-17 which was isolated from a patient with an acute gonococcal infection; these fractions were purified to eliminate the components of the outer membrane complex by affinity chromatography (Sepharose-anti-outer membrane complex antibody conjugates were used as the solid immunosorbent), and the resulting preparation was designated the purified ribosomal fraction. The purified ribosomal fraction was used to detect antibody activity in sera obtained from culture-positive asymptomatic carriers and healthy controls by a passive hemagglutination test. This passive hemagglutination test had a specificity of 100% for both sexes and sensitivities of 99.4 and 88.2% for female and male carriers, respectively, when an antibody titer of more than 1:3 was defined as abnormal. Absorption of the sera with nongonococcal organisms did not affect the antibody activity, and no significant difference in antigenicity among various N. gonorrhoeae strains was observed in ribosomal fractions. An enzyme-linked immunosorbent assay was also used to measure the relative amounts of specific antibodies to the purified ribosomal fraction, and this assay revealed that the anti-purified ribosomal fraction antibodies were immunoglobulin G.

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Section: Discussionmentioning

confidence: 98%

Passive hemagglutination test for detection of antibody to gonococcal ribosomal antigen in sera from patients with asymptomatic gonorrhea

Kita¹,

Kashiba²

1982

J Clin Microbiol

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“…Skin testing of the sensitized animals demonstrated greater activity of intact, crude 70S ribosomes than of fractions containing 50S and 30S ribosomal subunits. Further studies attempting further definition of the nature of gonococcal ribosomes as skin test antigens and as antigens in an in vitro correlate, the macrophage migration inhibition test, are described in the following paper (Judd and Koostra, 1976).…”

Section: Discussionmentioning

confidence: 99%

“…This study was designed to determine the sensitivity and specificity of gonococcal ribosomes in eliciting a cellmediated delayed hypersensitivity response in guineapigs sensitized with killed Neisseria. In the following paper (Judd and Koostra, 1976 of whole cells in 1-5 M saline. Sterile 1-5 M saline was also injected as a control.…”

Section: Introductionmentioning

confidence: 98%

Gonococcal ribosomes as skin test antigens. I. Preliminary tests of sensitivity and specificity.

Koostra¹,

Judd²,

Baker³

1976

Sexually Transmitted Infections

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SummaryRibosomes isolated from N. gonorrhoeae and N. meningitidis were used as skin test antigens in guinea-pigs which had been previously sensitized with killed Neisseria cells in incomplete Freund adjuvant. Intradermal injection of ribosomes from skin test dose N. gonorrhoeae into the skin of animals sensitized to the homologous organism was characterized by a specificity based upon ribosomal skin test dose 50's (STD50) sensitivity which was at least five-fold greater than that of the animals sensitized to the heterologous organism. The sensitized guinea-pigs had lost their skin-test reactivity within 3 months of the cessation of immunization but regained it immediately on reimmunization.The results of this model system indicate that gonococcal ribosomes may be used as antigens in skin testing or other testing modes in gonorrhoea.

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“…The CMI response was detected by using a crude soluble cellular antigen, crude lipopolysaccharide (LPS), and a ribonucleic acid protein antigen prepared from a local clinical isolate. Koostra et al (16) (14). Gonococcal ribosomal ribonucleic acid, ribosomal protein, cell, walls, and pili required higher concentrations to obtain a positive skin test than did intact ribosomes.…”

Section: Host Cellular Defenses In Gonococcal Infection CMI Response mentioning

confidence: 99%

Cellular immune responses during gonococcal and meningococcal infections

Cooper

Moticka

1989

Clin Microbiol Rev

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Gonococcal ribosomes as skin test antigens. II. Precision Of the Method, Attempts To Identify The Ribosomal Antigen, and Correlation with The Macrophage Migration Inhibition test.

Cited by 3 publications

References 14 publications

Passive hemagglutination test for detection of antibody to gonococcal ribosomal antigen in sera from patients with asymptomatic gonorrhea

Passive hemagglutination test for detection of antibody to gonococcal ribosomal antigen in sera from patients with asymptomatic gonorrhea

Gonococcal ribosomes as skin test antigens. I. Preliminary tests of sensitivity and specificity.

Cellular immune responses during gonococcal and meningococcal infections

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