Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels

Yeste, Marc; Estrada, Efrén; Casas, Isabel; Bonet, Sergi; Rodríguez-Gil, Joan E.

doi:10.1016/j.theriogenology.2013.01.008

Cited by 86 publications

(83 citation statements)

References 43 publications

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“…Significant decreases in the percentages of total and progressive motile spermatozoa and in the values of kinetic parameters are very apparent following freezethawing (Flores et al, 2009;Yeste et al, 2013aYeste et al, , b, 2014Estrada et al, 2014; Fig. 1).…”

Section: Sperm Motilitymentioning

confidence: 97%

“…1). However, the reduction of all these sperm motility parameters is greatly influenced by ejaculate freezability, as ejaculates with poor freezability present significantly lower motility values than those with good freezability (Yeste et al, 2013a.…”

Section: Sperm Motilitymentioning

confidence: 99%

“…In addition, there is an increase in DNA fragmentation, although this damage may not be apparent upon thawing (Flores et al, 2011) but rather it may be latent and only evident after incubating frozenthawed sperm at 37°C for at least 2 h (Alkmin et al, 2013;Yeste et al, 2013a, b). Cryopreservation of boar sperm also results in an increase in the disrupted disulphide bridges between cysteine radicals of protamines (Flores et al, 2011;Yeste et al, 2013a). This effect has not only been observed in porcine spermatozoa but also in other mammalian species, such as equine .…”

Section: Chromatin Integritymentioning

confidence: 99%

“…Flores et al (2010) found that cryopreservation decreased the capacity of mitochondria to produce ROS. With regard to differences between ejaculates of different freezability, Gómez-Fernández et al (2013) and Yeste et al (2013a) found that intracellular levels of ROS and proportions of sperm exhibiting membrane lipid peroxidation did not differ between ejaculates with different resilience to withstand freeze-thawing procedures.…”

Section: Mitochondrial Activity and Ros Generationmentioning

confidence: 99%

“…In the latter case, this high variability has led to classify boar ejaculates as good or poor freezability ejaculates (Watson, 1995;Casas et al, 2009), where freezability is defined as the ability to sustain freeze-thawing procedures (Yeste, 2016). The high individual variability is not only restricted to boars, but has also been observed between ejaculates coming from the same boar and even between fractions of the same ejaculate (Holt, 2000;Holt et al, 2005;Peña et al, 2006;Waterhouse et al, 2006;Yeste et al, 2013a).…”

Section: Ejaculate Resilience To Withstand Cryopreservationmentioning

confidence: 99%

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State-of-the-art of boar sperm preservation in liquid and frozen state

Yeste

2017

Anim Reprod

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Pig breeding is mainly conducted through Artificial Insemination (AI) in Western and developing countries. Apart from requiring specific catheters and trained staff, preserving boar semen in proper conditions is needed to ensure high reproductive performances. Although, at present, boar sperm may be preserved in liquid (15-17ºC) or frozen states, more than 95% of AIs are conducted using liquid semen. The present work reviews the state-the-art of these two preservation technologies. Thus, the composition and types of extenders for liquid-stored semen are discussed, together with the specific requirements for boar sperm, which are stored at 15-17ºC. Commercial extenders for liquid semen are compared and the effects of storage on sperm quality are also summarised. In the second part of the manuscript, the main features of boar sperm cryopreservation are described and reference to cryodamage is also made. These cryoinjuries mainly affect sperm motility, membrane permeability and chromatin integrity. Furthermore, the individual variability in the sperm resilience to withstand cryopreservation procedures is reviewed and a brief summary about freezability markers is also included. Final sections briefly discuss the improvement of freezing extenders with additives, such as seminal plasma and antioxidants, and highlight the relevance of using a proper AI technique to avoid dramatic drops in reproductive performance.

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Section: Sperm Motilitymentioning

confidence: 97%

Section: Sperm Motilitymentioning

confidence: 99%

Section: Chromatin Integritymentioning

confidence: 99%

Section: Mitochondrial Activity and Ros Generationmentioning

confidence: 99%

Section: Ejaculate Resilience To Withstand Cryopreservationmentioning

confidence: 99%

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State-of-the-art of boar sperm preservation in liquid and frozen state

Yeste

2017

Anim Reprod

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Artificial insemination with frozen‐thawed boar sperm

Yeste

Rodríguez-Gil

Bonet

2017

Molecular Reproduction Devel

Self Cite

106

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Artificial insemination with frozen-thawed semen in pigs is not a routine technique; its use is restricted to specific cases, such as preservation of valuable genetic material (germplasm banks), safety strategies in case of natural disasters, long-distance transport of sperm, and in combination with sex-sorting. Cryoinjuries resulting from freeze-thawing protocols are a major concern with regard to the fertilization capacity of the treated sperm, which is lower than that of liquid-stored semen. Here, we provide an overview of artificial insemination using cryopreserved sperm, and summarize the factors that influence cryopreservation success before, during, and after freeze-thaw (i.e., sperm selection before starting the cryopreservation process, holding time, use of cryoprotectants, and rates of freezing and thawing) and that are driving the identification of biomarkers to predict sensitivity to cryodamage. Three different artificial insemination techniques (conventional or intracervical; intrauterine; and deep intrauterine) are also discussed with regards to their relevance when using frozenthawed semen. Finally, we review the use of additives to freezing and thawing media, given reports that they may maintain and improve the quality and fertilizing capacity of frozen-thawed sperm. In sum, artificial insemination with frozen-thawed boar sperm can provide reasonable fertility outcomes, if freezable ejaculates, specific additives, and appropriate insemination techniques are used.State-of-the-art of artificial insemination with frozenthawed sperm still suffers from the negative impact that cryopreservation has on the sperm itself.

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